Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3876 and GFF3877 are separated by 2 nucleotides GFF3877 and GFF3878 overlap by 4 nucleotides GFF3878 and GFF3879 are separated by 2 nucleotides
GFF3876 - GDP-L-fucose synthetase (EC 1.1.1.271), at 152,252 to 153,217
GFF3876
GFF3877 - GDP-mannose mannosyl hydrolase (EC 3.6.1.-), at 153,220 to 153,693
GFF3877
GFF3878 - Colanic acid biosysnthesis glycosyl transferase WcaI, at 153,690 to 154,913
GFF3878
GFF3879 - Mannose-1-phosphate guanylyltransferase (GDP) (EC 2.7.7.22) / Mannose-6-phosphate isomerase (EC 5.3.1.8), at 154,916 to 156,352
GFF3879
Position (kb) 153
154
155 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 152.699 kb on + strand, within GFF3876 at 152.700 kb on - strand, within GFF3876 at 152.914 kb on + strand, within GFF3876 at 153.176 kb on + strand at 153.177 kb on - strand at 153.177 kb on - strand at 153.227 kb on + strand at 153.485 kb on - strand, within GFF3877 at 153.485 kb on - strand, within GFF3877 at 153.706 kb on - strand at 153.713 kb on + strand at 153.713 kb on + strand at 153.713 kb on + strand at 153.906 kb on + strand, within GFF3878 at 153.906 kb on + strand, within GFF3878 at 154.091 kb on - strand, within GFF3878 at 154.456 kb on + strand, within GFF3878 at 154.700 kb on + strand, within GFF3878 at 154.882 kb on + strand at 154.914 kb on + strand at 154.994 kb on + strand at 155.143 kb on + strand, within GFF3879 at 155.393 kb on + strand, within GFF3879 at 155.552 kb on + strand, within GFF3879 at 155.855 kb on + strand, within GFF3879
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 152,699 + GFF3876 0.46 +1.5 152,700 - GFF3876 0.46 -0.1 152,914 + GFF3876 0.69 +0.8 153,176 + +1.0 153,177 - -1.4 153,177 - -0.9 153,227 + +2.1 153,485 - GFF3877 0.56 +1.0 153,485 - GFF3877 0.56 -1.3 153,706 - +1.8 153,713 + +0.8 153,713 + -1.2 153,713 + -1.7 153,906 + GFF3878 0.18 +0.5 153,906 + GFF3878 0.18 -3.5 154,091 - GFF3878 0.33 +0.1 154,456 + GFF3878 0.63 -0.1 154,700 + GFF3878 0.83 -1.2 154,882 + -0.5 154,914 + +0.9 154,994 + -0.9 155,143 + GFF3879 0.16 -0.5 155,393 + GFF3879 0.33 -0.6 155,552 + GFF3879 0.44 -0.8 155,855 + GFF3879 0.65 +1.2
Or see this region's nucleotide sequence