Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3855 and GFF3856 are separated by 87 nucleotides GFF3856 and GFF3857 are separated by 236 nucleotides GFF3857 and GFF3858 are separated by 133 nucleotides
GFF3855 - Multidrug transporter MdtA, at 129,327 to 130,568
GFF3855
GFF3856 - hypothetical protein, at 130,656 to 130,772
GFF3856
GFF3857 - Putative heat shock protein YegD, at 131,009 to 132,361
GFF3857
GFF3858 - DNA-3-methyladenine glycosylase II (EC 3.2.2.21), at 132,495 to 133,364
GFF3858
GFF3859 - FIG00509877: hypothetical protein, at 133,332 to 136,328
GFF3859
Position (kb) 131
132
133 Strain fitness (log2 ratio) -2
-1
0
1
2 at 130.161 kb on - strand, within GFF3855 at 130.314 kb on - strand, within GFF3855 at 130.522 kb on - strand at 130.617 kb on + strand at 130.655 kb on + strand at 130.839 kb on - strand at 130.871 kb on - strand at 130.879 kb on + strand at 130.879 kb on + strand at 130.906 kb on - strand at 130.908 kb on + strand at 131.151 kb on + strand, within GFF3857 at 131.362 kb on + strand, within GFF3857 at 131.461 kb on - strand, within GFF3857 at 131.578 kb on + strand, within GFF3857 at 131.953 kb on + strand, within GFF3857 at 131.953 kb on + strand, within GFF3857 at 131.954 kb on - strand, within GFF3857 at 131.957 kb on + strand, within GFF3857 at 131.957 kb on + strand, within GFF3857 at 131.958 kb on - strand, within GFF3857 at 131.958 kb on - strand, within GFF3857 at 132.127 kb on + strand, within GFF3857 at 132.144 kb on + strand, within GFF3857 at 132.144 kb on + strand, within GFF3857 at 132.253 kb on + strand at 132.253 kb on + strand at 132.254 kb on - strand at 132.274 kb on + strand at 132.274 kb on + strand at 132.365 kb on - strand at 132.465 kb on - strand at 132.522 kb on + strand at 132.694 kb on + strand, within GFF3858 at 132.830 kb on + strand, within GFF3858 at 132.830 kb on + strand, within GFF3858 at 133.217 kb on + strand, within GFF3858 at 133.291 kb on - strand at 133.348 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 130,161 - GFF3855 0.67 +2.3 130,314 - GFF3855 0.79 -1.6 130,522 - -0.9 130,617 + +0.6 130,655 + -0.9 130,839 - -0.8 130,871 - -0.5 130,879 + +1.0 130,879 + +0.7 130,906 - -1.4 130,908 + -0.6 131,151 + GFF3857 0.10 +0.2 131,362 + GFF3857 0.26 -0.3 131,461 - GFF3857 0.33 +2.2 131,578 + GFF3857 0.42 -1.1 131,953 + GFF3857 0.70 +0.3 131,953 + GFF3857 0.70 -1.5 131,954 - GFF3857 0.70 -2.0 131,957 + GFF3857 0.70 -2.0 131,957 + GFF3857 0.70 -0.3 131,958 - GFF3857 0.70 -1.0 131,958 - GFF3857 0.70 +0.6 132,127 + GFF3857 0.83 +0.3 132,144 + GFF3857 0.84 -1.1 132,144 + GFF3857 0.84 +0.0 132,253 + -2.3 132,253 + -0.4 132,254 - +0.3 132,274 + -1.5 132,274 + -1.9 132,365 - -1.1 132,465 - -1.8 132,522 + +1.0 132,694 + GFF3858 0.23 +0.3 132,830 + GFF3858 0.39 +1.3 132,830 + GFF3858 0.39 -0.2 133,217 + GFF3858 0.83 +0.1 133,291 - +1.0 133,348 + +1.3
Or see this region's nucleotide sequence