Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3846 and GFF3847 are separated by 115 nucleotides GFF3847 and GFF3848 are separated by 10 nucleotides GFF3848 and GFF3849 overlap by 1 nucleotides
GFF3846 - Putative protease, at 113,807 to 115,168
GFF3846
GFF3847 - Putative inner membrane protein, at 115,284 to 117,395
GFF3847
GFF3848 - Putative inner membrane protein, at 117,406 to 118,122
GFF3848
GFF3849 - FIG074102: hypothetical protein, at 118,122 to 119,324
GFF3849
Position (kb)
115
116
117
118 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 114.384 kb on + strand, within GFF3846 at 114.384 kb on + strand, within GFF3846 at 114.384 kb on + strand, within GFF3846 at 114.385 kb on - strand, within GFF3846 at 114.444 kb on - strand, within GFF3846 at 114.566 kb on - strand, within GFF3846 at 114.567 kb on + strand, within GFF3846 at 114.812 kb on - strand, within GFF3846 at 114.866 kb on - strand, within GFF3846 at 115.052 kb on - strand at 115.052 kb on - strand at 115.068 kb on + strand at 115.087 kb on - strand at 115.285 kb on + strand at 115.285 kb on + strand at 115.286 kb on - strand at 115.339 kb on + strand at 115.339 kb on + strand at 115.466 kb on + strand at 115.467 kb on - strand at 115.569 kb on - strand, within GFF3847 at 115.581 kb on - strand, within GFF3847 at 115.631 kb on + strand, within GFF3847 at 115.754 kb on + strand, within GFF3847 at 115.896 kb on + strand, within GFF3847 at 115.899 kb on + strand, within GFF3847 at 115.919 kb on + strand, within GFF3847 at 115.958 kb on + strand, within GFF3847 at 116.045 kb on + strand, within GFF3847 at 116.045 kb on + strand, within GFF3847 at 116.052 kb on - strand, within GFF3847 at 116.053 kb on + strand, within GFF3847 at 116.322 kb on - strand, within GFF3847 at 116.400 kb on - strand, within GFF3847 at 116.423 kb on + strand, within GFF3847 at 116.424 kb on - strand, within GFF3847 at 116.494 kb on - strand, within GFF3847 at 116.603 kb on + strand, within GFF3847 at 116.606 kb on - strand, within GFF3847 at 116.606 kb on - strand, within GFF3847 at 116.621 kb on - strand, within GFF3847 at 117.251 kb on - strand at 117.309 kb on - strand at 117.366 kb on - strand at 117.542 kb on - strand, within GFF3848 at 117.561 kb on - strand, within GFF3848 at 117.619 kb on + strand, within GFF3848 at 117.749 kb on + strand, within GFF3848 at 117.749 kb on + strand, within GFF3848 at 117.818 kb on - strand, within GFF3848 at 117.903 kb on + strand, within GFF3848 at 117.904 kb on - strand, within GFF3848 at 118.164 kb on + strand at 118.165 kb on - strand at 118.292 kb on - strand, within GFF3849
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 114,384 + GFF3846 0.42 +0.7 114,384 + GFF3846 0.42 +2.7 114,384 + GFF3846 0.42 +0.1 114,385 - GFF3846 0.42 +0.5 114,444 - GFF3846 0.47 -0.4 114,566 - GFF3846 0.56 -2.8 114,567 + GFF3846 0.56 +1.7 114,812 - GFF3846 0.74 +0.4 114,866 - GFF3846 0.78 -0.8 115,052 - -0.5 115,052 - +1.6 115,068 + +1.8 115,087 - -0.4 115,285 + -0.4 115,285 + +0.9 115,286 - +0.4 115,339 + +1.6 115,339 + +0.3 115,466 + -3.1 115,467 - -0.8 115,569 - GFF3847 0.13 -2.5 115,581 - GFF3847 0.14 +1.2 115,631 + GFF3847 0.16 +1.5 115,754 + GFF3847 0.22 -0.6 115,896 + GFF3847 0.29 +1.8 115,899 + GFF3847 0.29 -1.5 115,919 + GFF3847 0.30 +0.4 115,958 + GFF3847 0.32 -0.3 116,045 + GFF3847 0.36 -0.2 116,045 + GFF3847 0.36 +0.4 116,052 - GFF3847 0.36 +0.2 116,053 + GFF3847 0.36 -1.1 116,322 - GFF3847 0.49 +0.9 116,400 - GFF3847 0.53 +1.5 116,423 + GFF3847 0.54 -1.5 116,424 - GFF3847 0.54 -0.6 116,494 - GFF3847 0.57 +0.1 116,603 + GFF3847 0.62 +0.3 116,606 - GFF3847 0.63 +0.8 116,606 - GFF3847 0.63 +0.3 116,621 - GFF3847 0.63 -0.8 117,251 - -0.2 117,309 - +0.6 117,366 - +0.8 117,542 - GFF3848 0.19 +0.3 117,561 - GFF3848 0.22 -0.7 117,619 + GFF3848 0.30 -0.4 117,749 + GFF3848 0.48 +0.6 117,749 + GFF3848 0.48 +0.5 117,818 - GFF3848 0.57 +1.5 117,903 + GFF3848 0.69 +0.8 117,904 - GFF3848 0.69 +1.2 118,164 + -0.9 118,165 - +0.3 118,292 - GFF3849 0.14 +0.3
Or see this region's nucleotide sequence