Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3807 and GFF3808 are separated by 44 nucleotides GFF3808 and GFF3809 are separated by 48 nucleotides GFF3809 and GFF3810 are separated by 158 nucleotides
GFF3807 - RND efflux system, outer membrane lipoprotein, NodT family, at 74,587 to 76,023
GFF3807
GFF3808 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 76,068 to 76,850
GFF3808
GFF3809 - DedA family inner membrane protein YohD, at 76,899 to 77,468
GFF3809
GFF3810 - Putative membrane protein, at 77,627 to 78,214
GFF3810
Position (kb)
76
77 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 75.267 kb on + strand, within GFF3807 at 75.268 kb on - strand, within GFF3807 at 75.727 kb on - strand, within GFF3807 at 75.752 kb on + strand, within GFF3807 at 75.753 kb on - strand, within GFF3807 at 75.981 kb on + strand at 76.052 kb on + strand at 76.053 kb on - strand at 76.059 kb on - strand at 76.067 kb on + strand at 76.088 kb on + strand at 76.108 kb on + strand at 76.311 kb on - strand, within GFF3808 at 76.311 kb on - strand, within GFF3808 at 76.347 kb on - strand, within GFF3808 at 76.376 kb on + strand, within GFF3808 at 76.463 kb on + strand, within GFF3808 at 76.464 kb on - strand, within GFF3808 at 76.470 kb on - strand, within GFF3808 at 76.470 kb on - strand, within GFF3808 at 76.521 kb on - strand, within GFF3808 at 76.727 kb on + strand, within GFF3808 at 76.727 kb on + strand, within GFF3808 at 76.728 kb on - strand, within GFF3808 at 76.779 kb on + strand at 76.779 kb on + strand at 76.779 kb on + strand at 77.165 kb on + strand, within GFF3809 at 77.191 kb on + strand, within GFF3809 at 77.235 kb on - strand, within GFF3809 at 77.272 kb on + strand, within GFF3809 at 77.290 kb on + strand, within GFF3809 at 77.291 kb on - strand, within GFF3809 at 77.440 kb on + strand at 77.440 kb on + strand at 77.450 kb on + strand at 77.472 kb on + strand at 77.473 kb on - strand at 77.495 kb on + strand at 77.495 kb on + strand at 77.495 kb on + strand at 77.496 kb on - strand at 77.496 kb on - strand at 77.496 kb on - strand at 77.496 kb on - strand at 77.546 kb on - strand at 77.637 kb on + strand at 77.637 kb on + strand at 77.637 kb on + strand at 77.637 kb on + strand at 77.638 kb on - strand at 77.713 kb on + strand, within GFF3810 at 77.713 kb on + strand, within GFF3810 at 77.714 kb on - strand, within GFF3810 at 77.714 kb on - strand, within GFF3810 at 77.808 kb on + strand, within GFF3810 at 77.809 kb on - strand, within GFF3810 at 77.809 kb on - strand, within GFF3810
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 75,267 + GFF3807 0.47 -1.9 75,268 - GFF3807 0.47 +0.0 75,727 - GFF3807 0.79 -0.1 75,752 + GFF3807 0.81 +0.2 75,753 - GFF3807 0.81 +0.0 75,981 + +1.1 76,052 + -0.6 76,053 - +0.4 76,059 - +0.1 76,067 + -0.8 76,088 + -0.7 76,108 + -1.4 76,311 - GFF3808 0.31 -0.7 76,311 - GFF3808 0.31 +0.2 76,347 - GFF3808 0.36 +0.1 76,376 + GFF3808 0.39 -0.1 76,463 + GFF3808 0.50 -0.4 76,464 - GFF3808 0.51 +0.6 76,470 - GFF3808 0.51 -0.2 76,470 - GFF3808 0.51 +0.3 76,521 - GFF3808 0.58 +0.8 76,727 + GFF3808 0.84 -0.5 76,727 + GFF3808 0.84 -1.7 76,728 - GFF3808 0.84 -0.4 76,779 + -0.4 76,779 + +0.1 76,779 + -0.1 77,165 + GFF3809 0.47 +0.7 77,191 + GFF3809 0.51 -0.0 77,235 - GFF3809 0.59 -0.3 77,272 + GFF3809 0.65 -3.2 77,290 + GFF3809 0.69 +0.6 77,291 - GFF3809 0.69 +0.4 77,440 + +0.1 77,440 + -1.0 77,450 + +0.0 77,472 + +0.4 77,473 - -0.4 77,495 + -0.7 77,495 + -1.4 77,495 + +0.3 77,496 - +0.9 77,496 - +2.0 77,496 - -0.2 77,496 - +0.6 77,546 - -2.1 77,637 + -0.4 77,637 + -1.1 77,637 + +1.7 77,637 + +0.0 77,638 - +0.4 77,713 + GFF3810 0.15 +0.8 77,713 + GFF3810 0.15 -0.4 77,714 - GFF3810 0.15 -0.6 77,714 - GFF3810 0.15 +0.9 77,808 + GFF3810 0.31 -1.3 77,809 - GFF3810 0.31 -0.5 77,809 - GFF3810 0.31 +1.3
Or see this region's nucleotide sequence