Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3787 and GFF3788 are separated by 498 nucleotides GFF3788 and GFF3789 are separated by 133 nucleotides
GFF3787 - Mgl repressor and galactose ultrainduction factor GalS, HTH-type transcriptional regulator, at 55,715 to 56,737
GFF3787
GFF3788 - Galactose/methyl galactoside ABC transport system, D-galactose-binding periplasmic protein MglB (TC 3.A.1.2.3), at 57,236 to 58,234
GFF3788
GFF3789 - Galactose/methyl galactoside ABC transport system, ATP-binding protein MglA (EC 3.6.3.17), at 58,368 to 59,888
GFF3789
Position (kb) 57
58
59 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 56.482 kb on - strand, within GFF3787 at 56.554 kb on + strand, within GFF3787 at 56.554 kb on + strand, within GFF3787 at 56.804 kb on + strand at 56.805 kb on - strand at 56.847 kb on + strand at 56.879 kb on + strand at 57.217 kb on + strand at 57.221 kb on - strand at 57.249 kb on + strand at 57.311 kb on - strand at 57.334 kb on + strand at 57.339 kb on - strand, within GFF3788 at 57.339 kb on - strand, within GFF3788 at 57.352 kb on + strand, within GFF3788 at 57.374 kb on - strand, within GFF3788 at 57.411 kb on + strand, within GFF3788 at 57.411 kb on + strand, within GFF3788 at 57.624 kb on - strand, within GFF3788 at 57.624 kb on - strand, within GFF3788 at 57.712 kb on + strand, within GFF3788 at 57.712 kb on + strand, within GFF3788 at 57.786 kb on - strand, within GFF3788 at 57.786 kb on - strand, within GFF3788 at 57.848 kb on - strand, within GFF3788 at 58.341 kb on - strand at 58.341 kb on - strand at 58.923 kb on - strand, within GFF3789 at 58.978 kb on - strand, within GFF3789 at 59.078 kb on + strand, within GFF3789 at 59.084 kb on + strand, within GFF3789 at 59.084 kb on + strand, within GFF3789
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 56,482 - GFF3787 0.75 +0.4 56,554 + GFF3787 0.82 -2.9 56,554 + GFF3787 0.82 -0.9 56,804 + +1.5 56,805 - -1.3 56,847 + +0.2 56,879 + +0.5 57,217 + -1.3 57,221 - +0.0 57,249 + -3.7 57,311 - -0.1 57,334 + +0.1 57,339 - GFF3788 0.10 +0.3 57,339 - GFF3788 0.10 -0.9 57,352 + GFF3788 0.12 -0.3 57,374 - GFF3788 0.14 +0.4 57,411 + GFF3788 0.18 -2.1 57,411 + GFF3788 0.18 +0.3 57,624 - GFF3788 0.39 +0.1 57,624 - GFF3788 0.39 +0.9 57,712 + GFF3788 0.48 -1.4 57,712 + GFF3788 0.48 +0.1 57,786 - GFF3788 0.55 +0.4 57,786 - GFF3788 0.55 -1.1 57,848 - GFF3788 0.61 -2.1 58,341 - -2.5 58,341 - +0.7 58,923 - GFF3789 0.36 -0.4 58,978 - GFF3789 0.40 +1.7 59,078 + GFF3789 0.47 +0.8 59,084 + GFF3789 0.47 -0.1 59,084 + GFF3789 0.47 +1.1
Or see this region's nucleotide sequence