Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3725 and GFF3726 are separated by 276 nucleotides GFF3726 and GFF3727 overlap by 1 nucleotides GFF3727 and GFF3728 overlap by 1 nucleotides GFF3728 and GFF3729 are separated by 7 nucleotides
GFF3725 - FKBP-type peptidyl-prolyl cis-trans isomerase FkpA precursor (EC 5.2.1.8), at 29,253 to 30,071
GFF3725
GFF3726 - YheO-like PAS domain, at 30,348 to 31,070
GFF3726
GFF3727 - tRNA 5-methylaminomethyl-2-thiouridine synthase TusD, at 31,070 to 31,456
GFF3727
GFF3728 - tRNA 5-methylaminomethyl-2-thiouridine synthase TusC, at 31,456 to 31,812
GFF3728
GFF3729 - tRNA 5-methylaminomethyl-2-thiouridine synthase TusB, at 31,820 to 32,107
GFF3729
Position (kb) 30
31
32 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 29.376 kb on - strand, within GFF3725 at 29.688 kb on - strand, within GFF3725 at 29.701 kb on - strand, within GFF3725 at 29.741 kb on + strand, within GFF3725 at 29.805 kb on + strand, within GFF3725 at 29.980 kb on + strand, within GFF3725 at 30.126 kb on + strand at 30.126 kb on + strand at 30.126 kb on + strand at 30.126 kb on + strand at 30.126 kb on + strand at 30.129 kb on + strand at 30.129 kb on + strand at 30.130 kb on - strand at 30.217 kb on + strand at 30.217 kb on + strand at 30.241 kb on - strand at 30.268 kb on - strand at 30.428 kb on + strand, within GFF3726 at 30.473 kb on + strand, within GFF3726 at 30.475 kb on + strand, within GFF3726 at 30.475 kb on + strand, within GFF3726 at 30.476 kb on - strand, within GFF3726 at 30.554 kb on + strand, within GFF3726 at 30.554 kb on + strand, within GFF3726 at 30.554 kb on + strand, within GFF3726 at 30.599 kb on + strand, within GFF3726 at 30.599 kb on + strand, within GFF3726 at 30.629 kb on + strand, within GFF3726 at 30.727 kb on + strand, within GFF3726 at 30.727 kb on + strand, within GFF3726 at 30.727 kb on + strand, within GFF3726 at 30.962 kb on + strand, within GFF3726 at 30.982 kb on + strand, within GFF3726 at 31.017 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 29,376 - GFF3725 0.15 -0.2 29,688 - GFF3725 0.53 -3.1 29,701 - GFF3725 0.55 -1.4 29,741 + GFF3725 0.60 -1.2 29,805 + GFF3725 0.67 -0.2 29,980 + GFF3725 0.89 +0.8 30,126 + +0.4 30,126 + -2.6 30,126 + -3.1 30,126 + +0.4 30,126 + +0.2 30,129 + -0.0 30,129 + -1.6 30,130 - +0.5 30,217 + -0.2 30,217 + -0.6 30,241 - -1.3 30,268 - +0.0 30,428 + GFF3726 0.11 -1.1 30,473 + GFF3726 0.17 -1.1 30,475 + GFF3726 0.18 +0.1 30,475 + GFF3726 0.18 +0.3 30,476 - GFF3726 0.18 -2.8 30,554 + GFF3726 0.28 +0.6 30,554 + GFF3726 0.28 +0.8 30,554 + GFF3726 0.28 +0.8 30,599 + GFF3726 0.35 +0.4 30,599 + GFF3726 0.35 -1.2 30,629 + GFF3726 0.39 +2.0 30,727 + GFF3726 0.52 -1.8 30,727 + GFF3726 0.52 +0.3 30,727 + GFF3726 0.52 -0.6 30,962 + GFF3726 0.85 -0.4 30,982 + GFF3726 0.88 +0.5 31,017 + +0.1
Or see this region's nucleotide sequence