Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3656 and GFF3657 are separated by 9 nucleotides GFF3657 and GFF3658 are separated by 136 nucleotides GFF3658 and GFF3659 are separated by 283 nucleotides GFF3659 and GFF3660 are separated by 75 nucleotides
GFF3656 - Pyruvate formate-lyase activating enzyme (EC 1.97.1.4), at 4,288 to 4,935
GFF3656
GFF3657 - hypothetical protein, at 4,945 to 5,076
GFF3657
GFF3658 - FIG01046987: hypothetical protein, at 5,213 to 5,377
GFF3658
GFF3659 - FIG01045244: hypothetical protein, at 5,661 to 6,620
GFF3659
GFF3660 - Pyruvate formate-lyase (EC 2.3.1.54), at 6,696 to 8,978
GFF3660
Position (kb) 5
6
7 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4.842 kb on - strand, within GFF3656 at 4.861 kb on + strand, within GFF3656 at 4.862 kb on - strand, within GFF3656 at 5.247 kb on - strand, within GFF3658 at 5.247 kb on - strand, within GFF3658 at 5.247 kb on - strand, within GFF3658 at 5.355 kb on + strand, within GFF3658 at 5.366 kb on + strand at 5.367 kb on - strand at 5.367 kb on - strand at 5.755 kb on + strand at 5.755 kb on + strand at 5.874 kb on - strand, within GFF3659 at 6.281 kb on + strand, within GFF3659 at 6.932 kb on + strand, within GFF3660 at 6.980 kb on + strand, within GFF3660 at 7.019 kb on + strand, within GFF3660 at 7.142 kb on - strand, within GFF3660 at 7.234 kb on - strand, within GFF3660 at 7.234 kb on - strand, within GFF3660 at 7.354 kb on + strand, within GFF3660 at 7.508 kb on - strand, within GFF3660 at 7.508 kb on - strand, within GFF3660
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 4,842 - GFF3656 0.85 -2.0 4,861 + GFF3656 0.88 +0.5 4,862 - GFF3656 0.89 +0.4 5,247 - GFF3658 0.21 +0.3 5,247 - GFF3658 0.21 +0.3 5,247 - GFF3658 0.21 -2.5 5,355 + GFF3658 0.86 +0.5 5,366 + -1.0 5,367 - -0.4 5,367 - +0.1 5,755 + -2.4 5,755 + +0.5 5,874 - GFF3659 0.22 -0.5 6,281 + GFF3659 0.65 -1.2 6,932 + GFF3660 0.10 +1.1 6,980 + GFF3660 0.12 -2.3 7,019 + GFF3660 0.14 +0.6 7,142 - GFF3660 0.20 -2.5 7,234 - GFF3660 0.24 -0.4 7,234 - GFF3660 0.24 +1.5 7,354 + GFF3660 0.29 -1.5 7,508 - GFF3660 0.36 -2.8 7,508 - GFF3660 0.36 +0.2
Or see this region's nucleotide sequence