Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3653 and GFF3654 are separated by 349 nucleotides GFF3654 and GFF3655 are separated by 217 nucleotides
GFF3653 - Anaerobic dimethyl sulfoxide reductase chain C (EC 1.8.99.-), at 25 to 858
GFF3653
GFF3654 - Hypothetical MFS-type transporter protein YcaD, at 1,208 to 2,356
GFF3654
GFF3655 - Probable transport protein, at 2,574 to 3,995
GFF3655
Position (kb) 1
2
3 Strain fitness (log2 ratio) -2
-1
0
1
2 at 0.214 kb on - strand, within GFF3653 at 0.403 kb on + strand, within GFF3653 at 0.403 kb on + strand, within GFF3653 at 0.403 kb on + strand, within GFF3653 at 0.434 kb on - strand, within GFF3653 at 0.684 kb on + strand, within GFF3653 at 0.685 kb on - strand, within GFF3653 at 0.732 kb on - strand, within GFF3653 at 0.817 kb on + strand at 0.817 kb on + strand at 0.818 kb on - strand at 0.931 kb on - strand at 0.931 kb on - strand at 0.931 kb on - strand at 0.932 kb on + strand at 0.955 kb on - strand at 1.075 kb on + strand at 1.356 kb on - strand, within GFF3654 at 1.399 kb on - strand, within GFF3654 at 1.610 kb on + strand, within GFF3654 at 1.611 kb on - strand, within GFF3654 at 1.626 kb on - strand, within GFF3654 at 1.626 kb on - strand, within GFF3654 at 1.627 kb on + strand, within GFF3654 at 1.627 kb on + strand, within GFF3654 at 1.628 kb on - strand, within GFF3654 at 1.732 kb on - strand, within GFF3654 at 1.863 kb on + strand, within GFF3654 at 1.864 kb on - strand, within GFF3654 at 1.881 kb on + strand, within GFF3654 at 1.883 kb on + strand, within GFF3654 at 2.023 kb on - strand, within GFF3654 at 2.191 kb on - strand, within GFF3654 at 2.196 kb on - strand, within GFF3654 at 2.297 kb on + strand at 2.395 kb on - strand at 2.421 kb on + strand at 2.543 kb on + strand at 2.667 kb on + strand at 2.667 kb on + strand at 2.668 kb on - strand at 2.722 kb on - strand, within GFF3655 at 2.733 kb on + strand, within GFF3655 at 2.792 kb on + strand, within GFF3655 at 2.893 kb on + strand, within GFF3655 at 2.949 kb on - strand, within GFF3655 at 3.079 kb on + strand, within GFF3655 at 3.113 kb on + strand, within GFF3655 at 3.138 kb on - strand, within GFF3655 at 3.181 kb on - strand, within GFF3655 at 3.181 kb on - strand, within GFF3655 at 3.182 kb on + strand, within GFF3655 at 3.197 kb on + strand, within GFF3655 at 3.197 kb on + strand, within GFF3655 at 3.197 kb on + strand, within GFF3655 at 3.197 kb on + strand, within GFF3655 at 3.198 kb on - strand, within GFF3655 at 3.241 kb on - strand, within GFF3655 at 3.241 kb on - strand, within GFF3655
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 214 - GFF3653 0.23 +0.1 403 + GFF3653 0.45 +0.6 403 + GFF3653 0.45 -1.0 403 + GFF3653 0.45 +0.7 434 - GFF3653 0.49 -0.4 684 + GFF3653 0.79 +0.2 685 - GFF3653 0.79 -1.5 732 - GFF3653 0.85 -0.7 817 + +1.2 817 + -0.3 818 - +0.7 931 - -1.0 931 - +0.1 931 - -0.1 932 + +0.1 955 - +0.1 1,075 + -0.3 1,356 - GFF3654 0.13 -1.2 1,399 - GFF3654 0.17 +2.1 1,610 + GFF3654 0.35 +0.3 1,611 - GFF3654 0.35 +0.2 1,626 - GFF3654 0.36 +1.1 1,626 - GFF3654 0.36 -0.8 1,627 + GFF3654 0.36 +1.1 1,627 + GFF3654 0.36 +0.4 1,628 - GFF3654 0.37 +0.4 1,732 - GFF3654 0.46 -1.5 1,863 + GFF3654 0.57 -0.0 1,864 - GFF3654 0.57 -1.6 1,881 + GFF3654 0.59 +0.1 1,883 + GFF3654 0.59 -0.5 2,023 - GFF3654 0.71 -0.9 2,191 - GFF3654 0.86 +2.5 2,196 - GFF3654 0.86 +0.2 2,297 + +0.1 2,395 - -2.5 2,421 + +0.4 2,543 + +0.2 2,667 + +0.3 2,667 + +0.1 2,668 - -1.8 2,722 - GFF3655 0.10 +0.4 2,733 + GFF3655 0.11 -0.7 2,792 + GFF3655 0.15 -0.3 2,893 + GFF3655 0.22 +0.3 2,949 - GFF3655 0.26 +1.0 3,079 + GFF3655 0.36 -0.5 3,113 + GFF3655 0.38 +0.5 3,138 - GFF3655 0.40 -1.1 3,181 - GFF3655 0.43 -0.3 3,181 - GFF3655 0.43 +1.0 3,182 + GFF3655 0.43 +0.4 3,197 + GFF3655 0.44 -1.3 3,197 + GFF3655 0.44 +0.4 3,197 + GFF3655 0.44 -1.5 3,197 + GFF3655 0.44 -0.2 3,198 - GFF3655 0.44 -0.4 3,241 - GFF3655 0.47 +0.5 3,241 - GFF3655 0.47 +0.2
Or see this region's nucleotide sequence