Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3447 and GFF3448 are separated by 27 nucleotides GFF3448 and GFF3449 are separated by 90 nucleotides GFF3449 and GFF3450 are separated by 68 nucleotides
GFF3447 - Candidate zinc-binding lipoprotein ZinT, at 207,889 to 208,539
GFF3447
GFF3448 - 'Streptomycin 3''-O-adenylyltransferase (EC 2.7.7.47) @ Spectinomycin 9-O-adenylyltransferase' transl_table=11, at 208,567 to 209,436
GFF3448
GFF3449 - Putative arylsulfatase regulatory protein, at 209,527 to 210,174
GFF3449
GFF3450 - Transcriptional regulator, MerR family, at 210,243 to 211,091
GFF3450
Position (kb) 209
210
211 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 208.537 kb on + strand at 208.538 kb on - strand at 208.538 kb on - strand at 208.538 kb on - strand at 208.619 kb on + strand at 208.746 kb on - strand, within GFF3448 at 209.045 kb on + strand, within GFF3448 at 209.073 kb on + strand, within GFF3448 at 209.198 kb on - strand, within GFF3448 at 209.198 kb on - strand, within GFF3448 at 209.230 kb on + strand, within GFF3448 at 209.233 kb on - strand, within GFF3448 at 209.306 kb on - strand, within GFF3448 at 209.338 kb on + strand, within GFF3448 at 209.339 kb on - strand, within GFF3448 at 209.434 kb on + strand at 209.434 kb on + strand at 209.435 kb on - strand at 209.435 kb on - strand at 209.484 kb on - strand at 209.513 kb on - strand at 209.751 kb on + strand, within GFF3449 at 210.248 kb on + strand at 210.255 kb on - strand at 210.704 kb on - strand, within GFF3450 at 210.740 kb on + strand, within GFF3450 at 210.740 kb on + strand, within GFF3450 at 210.800 kb on + strand, within GFF3450 at 210.856 kb on + strand, within GFF3450 at 211.124 kb on + strand at 211.125 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 208,537 + -0.5 208,538 - +0.5 208,538 - +0.5 208,538 - +0.5 208,619 + -0.9 208,746 - GFF3448 0.21 -0.2 209,045 + GFF3448 0.55 +0.9 209,073 + GFF3448 0.58 -1.2 209,198 - GFF3448 0.73 -0.6 209,198 - GFF3448 0.73 -1.1 209,230 + GFF3448 0.76 -1.6 209,233 - GFF3448 0.77 -1.3 209,306 - GFF3448 0.85 +0.3 209,338 + GFF3448 0.89 -0.2 209,339 - GFF3448 0.89 +0.6 209,434 + +2.6 209,434 + -1.7 209,435 - +0.5 209,435 - -0.1 209,484 - -3.0 209,513 - -0.2 209,751 + GFF3449 0.35 -1.0 210,248 + -0.1 210,255 - -0.5 210,704 - GFF3450 0.54 -2.2 210,740 + GFF3450 0.59 +0.2 210,740 + GFF3450 0.59 +0.1 210,800 + GFF3450 0.66 +0.3 210,856 + GFF3450 0.72 +0.3 211,124 + -0.2 211,125 - +1.0
Or see this region's nucleotide sequence