Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3304 and GFF3305 are separated by 105 nucleotides GFF3305 and GFF3306 are separated by 284 nucleotides
GFF3304 - Phosphate starvation-inducible protein PhoH, predicted ATPase, at 76,248 to 77,036
GFF3304
GFF3305 - Sialic acid utilization regulator, RpiR family, at 77,142 to 78,023
GFF3305
GFF3306 - Predicted sialic acid transporter, at 78,308 to 79,804
GFF3306
Position (kb) 77
78
79 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 76.153 kb on - strand at 76.160 kb on - strand at 76.222 kb on + strand at 76.223 kb on - strand at 76.223 kb on - strand at 76.224 kb on + strand at 76.228 kb on + strand at 76.229 kb on - strand at 76.322 kb on + strand at 76.406 kb on + strand, within GFF3304 at 76.705 kb on - strand, within GFF3304 at 76.753 kb on - strand, within GFF3304 at 76.754 kb on + strand, within GFF3304 at 76.767 kb on - strand, within GFF3304 at 77.035 kb on - strand at 77.040 kb on - strand at 77.224 kb on + strand at 77.337 kb on - strand, within GFF3305 at 77.468 kb on + strand, within GFF3305 at 77.484 kb on - strand, within GFF3305 at 77.650 kb on - strand, within GFF3305 at 77.669 kb on + strand, within GFF3305 at 77.669 kb on + strand, within GFF3305 at 77.815 kb on + strand, within GFF3305 at 77.885 kb on + strand, within GFF3305 at 77.886 kb on - strand, within GFF3305 at 78.023 kb on + strand at 78.120 kb on + strand at 78.121 kb on - strand at 78.121 kb on - strand at 78.124 kb on + strand at 78.160 kb on + strand at 78.206 kb on + strand at 78.235 kb on - strand at 78.645 kb on - strand, within GFF3306 at 78.674 kb on - strand, within GFF3306 at 78.674 kb on - strand, within GFF3306 at 78.984 kb on - strand, within GFF3306
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 76,153 - +0.5 76,160 - +3.1 76,222 + +0.7 76,223 - -0.3 76,223 - -2.8 76,224 + -1.1 76,228 + +0.3 76,229 - -0.3 76,322 + -0.1 76,406 + GFF3304 0.20 +0.6 76,705 - GFF3304 0.58 +0.5 76,753 - GFF3304 0.64 -1.3 76,754 + GFF3304 0.64 +0.2 76,767 - GFF3304 0.66 +0.6 77,035 - -0.2 77,040 - +0.4 77,224 + -0.3 77,337 - GFF3305 0.22 -0.6 77,468 + GFF3305 0.37 +0.5 77,484 - GFF3305 0.39 +0.1 77,650 - GFF3305 0.58 -1.5 77,669 + GFF3305 0.60 +0.1 77,669 + GFF3305 0.60 -0.9 77,815 + GFF3305 0.76 +0.8 77,885 + GFF3305 0.84 +0.8 77,886 - GFF3305 0.84 -1.3 78,023 + +0.5 78,120 + -0.3 78,121 - +1.1 78,121 - -1.7 78,124 + -1.2 78,160 + +0.4 78,206 + -0.9 78,235 - +1.3 78,645 - GFF3306 0.23 +0.4 78,674 - GFF3306 0.24 +0.1 78,674 - GFF3306 0.24 -0.5 78,984 - GFF3306 0.45 +0.2
Or see this region's nucleotide sequence