Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3280 and GFF3281 are separated by 9 nucleotides GFF3281 and GFF3282 are separated by 142 nucleotides GFF3282 and GFF3283 are separated by 10 nucleotides GFF3283 and GFF3284 are separated by 71 nucleotides
GFF3280 - 3,4-dihydroxyphenylacetate 2,3-dioxygenase (EC 1.13.11.15), at 52,841 to 53,692
GFF3280
GFF3281 - 5-carboxymethyl-2-hydroxymuconate delta-isomerase (EC 5.3.3.10), at 53,702 to 54,082
GFF3281
GFF3282 - 2-oxo-hepta-3-ene-1,7-dioic acid hydratase (EC 4.2.-.-), at 54,225 to 55,028
GFF3282
GFF3283 - 2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase (EC 4.1.2.-), at 55,039 to 55,830
GFF3283
GFF3284 - 4-hydroxyphenylacetate symporter, major facilitator superfamily (MFS), at 55,902 to 57,278
GFF3284
Position (kb) 54
55
56 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 53.238 kb on - strand, within GFF3280 at 53.351 kb on + strand, within GFF3280 at 53.352 kb on - strand, within GFF3280 at 53.607 kb on - strand at 53.713 kb on + strand at 54.048 kb on - strand at 54.072 kb on + strand at 54.212 kb on - strand at 54.224 kb on + strand at 54.267 kb on - strand at 54.329 kb on + strand, within GFF3282 at 54.342 kb on + strand, within GFF3282 at 54.343 kb on - strand, within GFF3282 at 54.462 kb on + strand, within GFF3282 at 54.463 kb on - strand, within GFF3282 at 54.622 kb on - strand, within GFF3282 at 54.897 kb on + strand, within GFF3282 at 55.130 kb on + strand, within GFF3283 at 55.313 kb on + strand, within GFF3283 at 55.381 kb on - strand, within GFF3283 at 55.469 kb on + strand, within GFF3283 at 55.548 kb on + strand, within GFF3283 at 55.643 kb on + strand, within GFF3283 at 55.702 kb on + strand, within GFF3283 at 55.870 kb on + strand at 55.876 kb on - strand at 55.992 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 53,238 - GFF3280 0.47 +0.9 53,351 + GFF3280 0.60 -1.0 53,352 - GFF3280 0.60 +0.6 53,607 - +0.5 53,713 + +0.5 54,048 - -0.5 54,072 + -2.5 54,212 - +0.4 54,224 + +0.3 54,267 - -4.1 54,329 + GFF3282 0.13 -0.2 54,342 + GFF3282 0.15 +1.2 54,343 - GFF3282 0.15 -2.3 54,462 + GFF3282 0.29 -2.0 54,463 - GFF3282 0.30 -1.7 54,622 - GFF3282 0.49 +0.2 54,897 + GFF3282 0.84 -0.3 55,130 + GFF3283 0.11 -0.1 55,313 + GFF3283 0.35 -1.3 55,381 - GFF3283 0.43 -0.1 55,469 + GFF3283 0.54 +1.8 55,548 + GFF3283 0.64 -0.3 55,643 + GFF3283 0.76 +0.9 55,702 + GFF3283 0.84 +0.1 55,870 + +0.0 55,876 - -1.7 55,992 - -1.3
Or see this region's nucleotide sequence