Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3208 and GFF3209 are separated by 65 nucleotides GFF3209 and GFF3210 are separated by 20 nucleotides
GFF3208 - Acetate kinase (EC 2.7.2.1), at 213,895 to 215,103
GFF3208
GFF3209 - L-threonine transporter, anaerobically inducible, at 215,169 to 216,500
GFF3209
GFF3210 - Threonine dehydratase, catabolic (EC 4.3.1.19), at 216,521 to 217,510
GFF3210
Position (kb) 215
216
217 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 214.443 kb on + strand, within GFF3208 at 214.637 kb on - strand, within GFF3208 at 214.807 kb on - strand, within GFF3208 at 214.807 kb on - strand, within GFF3208 at 214.826 kb on - strand, within GFF3208 at 214.826 kb on - strand, within GFF3208 at 215.003 kb on - strand at 215.105 kb on + strand at 215.157 kb on + strand at 215.157 kb on + strand at 215.271 kb on - strand at 215.368 kb on - strand, within GFF3209 at 215.411 kb on + strand, within GFF3209 at 215.448 kb on - strand, within GFF3209 at 215.551 kb on + strand, within GFF3209 at 215.684 kb on + strand, within GFF3209 at 215.721 kb on - strand, within GFF3209 at 215.721 kb on - strand, within GFF3209 at 215.721 kb on - strand, within GFF3209 at 215.750 kb on + strand, within GFF3209 at 215.786 kb on - strand, within GFF3209 at 215.799 kb on - strand, within GFF3209 at 215.997 kb on + strand, within GFF3209 at 216.004 kb on + strand, within GFF3209 at 216.141 kb on - strand, within GFF3209 at 216.144 kb on - strand, within GFF3209 at 216.158 kb on - strand, within GFF3209 at 216.190 kb on + strand, within GFF3209 at 216.208 kb on - strand, within GFF3209 at 216.248 kb on + strand, within GFF3209 at 216.307 kb on + strand, within GFF3209 at 216.307 kb on + strand, within GFF3209 at 216.308 kb on - strand, within GFF3209 at 216.322 kb on + strand, within GFF3209 at 216.478 kb on - strand at 216.486 kb on - strand at 216.492 kb on - strand at 216.673 kb on + strand, within GFF3210 at 217.313 kb on - strand, within GFF3210 at 217.313 kb on - strand, within GFF3210 at 217.435 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 214,443 + GFF3208 0.45 +0.8 214,637 - GFF3208 0.61 +0.9 214,807 - GFF3208 0.75 +0.5 214,807 - GFF3208 0.75 -1.2 214,826 - GFF3208 0.77 +0.3 214,826 - GFF3208 0.77 -1.0 215,003 - -0.3 215,105 + -1.5 215,157 + +0.5 215,157 + +0.5 215,271 - -0.6 215,368 - GFF3209 0.15 +2.7 215,411 + GFF3209 0.18 -0.2 215,448 - GFF3209 0.21 +0.1 215,551 + GFF3209 0.29 -1.4 215,684 + GFF3209 0.39 -0.8 215,721 - GFF3209 0.41 -0.2 215,721 - GFF3209 0.41 -0.8 215,721 - GFF3209 0.41 +2.2 215,750 + GFF3209 0.44 +0.3 215,786 - GFF3209 0.46 +1.3 215,799 - GFF3209 0.47 -1.1 215,997 + GFF3209 0.62 +0.3 216,004 + GFF3209 0.63 -2.3 216,141 - GFF3209 0.73 -0.0 216,144 - GFF3209 0.73 -0.9 216,158 - GFF3209 0.74 +0.8 216,190 + GFF3209 0.77 -0.1 216,208 - GFF3209 0.78 -3.5 216,248 + GFF3209 0.81 +0.2 216,307 + GFF3209 0.85 -0.9 216,307 + GFF3209 0.85 +1.2 216,308 - GFF3209 0.86 +0.4 216,322 + GFF3209 0.87 +0.2 216,478 - +0.3 216,486 - -1.5 216,492 - +0.1 216,673 + GFF3210 0.15 -0.4 217,313 - GFF3210 0.80 -0.1 217,313 - GFF3210 0.80 -0.1 217,435 - +0.8
Or see this region's nucleotide sequence