Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3189 and GFF3190 are separated by 272 nucleotides GFF3190 and GFF3191 are separated by 254 nucleotides
GFF3189 - Possible oxidoreductase, at 196,141 to 197,139
GFF3189
GFF3190 - Integral membrane protein TerC, at 197,412 to 198,380
GFF3190
GFF3191 - Sodium:dicarboxylate symporter, at 198,635 to 199,879
GFF3191
Position (kb) 197
198
199 Strain fitness (log2 ratio) -2
-1
0
1
2 at 196.569 kb on + strand, within GFF3189 at 196.581 kb on - strand, within GFF3189 at 196.586 kb on - strand, within GFF3189 at 196.672 kb on - strand, within GFF3189 at 196.766 kb on + strand, within GFF3189 at 196.966 kb on - strand, within GFF3189 at 196.966 kb on - strand, within GFF3189 at 196.966 kb on - strand, within GFF3189 at 196.968 kb on + strand, within GFF3189 at 196.972 kb on - strand, within GFF3189 at 196.982 kb on - strand, within GFF3189 at 197.046 kb on - strand at 197.148 kb on - strand at 197.216 kb on - strand at 197.241 kb on + strand at 197.241 kb on + strand at 197.242 kb on - strand at 197.242 kb on - strand at 197.345 kb on - strand at 197.350 kb on + strand at 197.400 kb on + strand at 197.400 kb on + strand at 197.714 kb on + strand, within GFF3190 at 197.715 kb on - strand, within GFF3190 at 197.844 kb on + strand, within GFF3190 at 197.996 kb on + strand, within GFF3190 at 198.012 kb on + strand, within GFF3190 at 198.012 kb on + strand, within GFF3190 at 198.081 kb on - strand, within GFF3190 at 198.081 kb on - strand, within GFF3190 at 198.122 kb on - strand, within GFF3190 at 198.148 kb on + strand, within GFF3190 at 198.211 kb on - strand, within GFF3190 at 198.267 kb on + strand, within GFF3190 at 198.268 kb on - strand, within GFF3190 at 198.299 kb on - strand at 198.541 kb on + strand at 198.543 kb on + strand at 198.568 kb on - strand at 198.620 kb on + strand at 198.661 kb on - strand at 198.831 kb on + strand, within GFF3191 at 199.012 kb on - strand, within GFF3191 at 199.012 kb on - strand, within GFF3191 at 199.075 kb on + strand, within GFF3191 at 199.076 kb on - strand, within GFF3191 at 199.079 kb on + strand, within GFF3191 at 199.080 kb on - strand, within GFF3191 at 199.080 kb on - strand, within GFF3191 at 199.080 kb on - strand, within GFF3191 at 199.133 kb on - strand, within GFF3191 at 199.309 kb on + strand, within GFF3191
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 196,569 + GFF3189 0.43 +0.2 196,581 - GFF3189 0.44 +1.1 196,586 - GFF3189 0.45 -1.5 196,672 - GFF3189 0.53 +0.2 196,766 + GFF3189 0.63 +0.7 196,966 - GFF3189 0.83 -1.4 196,966 - GFF3189 0.83 +0.7 196,966 - GFF3189 0.83 +0.1 196,968 + GFF3189 0.83 +0.1 196,972 - GFF3189 0.83 -0.2 196,982 - GFF3189 0.84 +1.1 197,046 - +1.9 197,148 - -0.9 197,216 - +0.1 197,241 + +0.3 197,241 + -0.4 197,242 - -1.5 197,242 - +0.2 197,345 - +1.5 197,350 + +0.2 197,400 + -0.1 197,400 + -1.1 197,714 + GFF3190 0.31 -0.3 197,715 - GFF3190 0.31 +0.5 197,844 + GFF3190 0.45 +0.0 197,996 + GFF3190 0.60 +0.8 198,012 + GFF3190 0.62 -1.7 198,012 + GFF3190 0.62 +0.1 198,081 - GFF3190 0.69 -2.2 198,081 - GFF3190 0.69 +1.8 198,122 - GFF3190 0.73 -0.3 198,148 + GFF3190 0.76 -0.5 198,211 - GFF3190 0.82 +1.8 198,267 + GFF3190 0.88 -2.5 198,268 - GFF3190 0.88 -0.5 198,299 - -0.6 198,541 + +1.1 198,543 + +1.6 198,568 - -0.9 198,620 + +0.8 198,661 - +0.2 198,831 + GFF3191 0.16 -1.7 199,012 - GFF3191 0.30 +0.3 199,012 - GFF3191 0.30 -1.0 199,075 + GFF3191 0.35 +0.7 199,076 - GFF3191 0.35 +0.0 199,079 + GFF3191 0.36 +0.2 199,080 - GFF3191 0.36 -0.7 199,080 - GFF3191 0.36 +1.6 199,080 - GFF3191 0.36 +0.2 199,133 - GFF3191 0.40 +0.3 199,309 + GFF3191 0.54 -0.1
Or see this region's nucleotide sequence