Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3144 and GFF3145 are separated by 27 nucleotides GFF3145 and GFF3146 overlap by 1 nucleotides GFF3146 and GFF3147 are separated by 24 nucleotides GFF3147 and GFF3148 overlap by 4 nucleotides
GFF3144 - Topoisomerase IV subunit B (EC 5.99.1.-), at 149,097 to 150,989
GFF3144
GFF3145 - Putative esterase, FIGfam005057, at 151,017 to 151,598
GFF3145
GFF3146 - '3',5'-cyclic-nucleotide phosphodiesterase (EC 3.1.4.17)' transl_table=11, at 151,598 to 152,425
GFF3146
GFF3147 - FIG074102: hypothetical protein, at 152,450 to 152,872
GFF3147
GFF3148 - ADP-ribose pyrophosphatase (EC 3.6.1.13), at 152,869 to 153,501
GFF3148
Position (kb) 151
152
153 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 151.127 kb on - strand, within GFF3145 at 151.127 kb on - strand, within GFF3145 at 151.255 kb on + strand, within GFF3145 at 151.256 kb on - strand, within GFF3145 at 151.256 kb on - strand, within GFF3145 at 151.256 kb on - strand, within GFF3145 at 151.352 kb on + strand, within GFF3145 at 151.715 kb on - strand, within GFF3146 at 151.816 kb on + strand, within GFF3146 at 152.436 kb on + strand at 152.568 kb on + strand, within GFF3147 at 152.664 kb on + strand, within GFF3147 at 152.664 kb on + strand, within GFF3147 at 152.704 kb on + strand, within GFF3147 at 152.744 kb on + strand, within GFF3147 at 153.007 kb on - strand, within GFF3148 at 153.037 kb on - strand, within GFF3148 at 153.076 kb on - strand, within GFF3148 at 153.076 kb on - strand, within GFF3148 at 153.113 kb on + strand, within GFF3148 at 153.205 kb on + strand, within GFF3148
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 151,127 - GFF3145 0.19 +0.7 151,127 - GFF3145 0.19 +1.0 151,255 + GFF3145 0.41 -1.4 151,256 - GFF3145 0.41 -1.5 151,256 - GFF3145 0.41 -0.9 151,256 - GFF3145 0.41 -0.7 151,352 + GFF3145 0.58 +1.3 151,715 - GFF3146 0.14 +1.3 151,816 + GFF3146 0.26 +2.1 152,436 + +1.0 152,568 + GFF3147 0.28 +1.5 152,664 + GFF3147 0.51 +1.5 152,664 + GFF3147 0.51 -1.6 152,704 + GFF3147 0.60 -3.6 152,744 + GFF3147 0.70 +0.6 153,007 - GFF3148 0.22 -1.8 153,037 - GFF3148 0.27 +0.0 153,076 - GFF3148 0.33 -0.6 153,076 - GFF3148 0.33 -1.5 153,113 + GFF3148 0.39 +0.5 153,205 + GFF3148 0.53 -0.7
Or see this region's nucleotide sequence