Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3112 and GFF3113 are separated by 279 nucleotides GFF3113 and GFF3114 are separated by 103 nucleotides GFF3114 and GFF3115 overlap by 14 nucleotides
GFF3112 - Putative membrane protein, at 121,409 to 121,903
GFF3112
GFF3113 - Putative ATP-dependent RNA helicase-like protein, at 122,183 to 122,686
GFF3113
GFF3114 - FIG01045310: hypothetical protein, at 122,790 to 123,200
GFF3114
GFF3115 - putative cytoplasmic protein, at 123,187 to 123,591
GFF3115
Position (kb) 122
123 Strain fitness (log2 ratio) -2
-1
0
1
2 at 121.183 kb on + strand at 121.183 kb on + strand at 121.184 kb on - strand at 121.291 kb on - strand at 121.497 kb on - strand, within GFF3112 at 121.692 kb on - strand, within GFF3112 at 121.876 kb on - strand at 121.880 kb on + strand at 121.881 kb on - strand at 121.938 kb on - strand at 121.939 kb on + strand at 121.939 kb on + strand at 121.984 kb on - strand at 122.169 kb on + strand at 122.184 kb on + strand at 122.345 kb on + strand, within GFF3113 at 122.346 kb on - strand, within GFF3113 at 122.476 kb on - strand, within GFF3113 at 122.476 kb on - strand, within GFF3113 at 123.376 kb on + strand, within GFF3115 at 123.376 kb on + strand, within GFF3115 at 123.376 kb on + strand, within GFF3115 at 123.376 kb on + strand, within GFF3115 at 123.376 kb on + strand, within GFF3115 at 123.376 kb on + strand, within GFF3115 at 123.377 kb on - strand, within GFF3115 at 123.377 kb on - strand, within GFF3115 at 123.515 kb on + strand, within GFF3115 at 123.515 kb on + strand, within GFF3115 at 123.516 kb on - strand, within GFF3115 at 123.602 kb on + strand at 123.606 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 121,183 + +0.3 121,183 + +0.4 121,184 - +1.6 121,291 - +0.2 121,497 - GFF3112 0.18 +0.3 121,692 - GFF3112 0.57 -1.1 121,876 - +0.1 121,880 + -2.4 121,881 - +0.9 121,938 - -1.5 121,939 + -1.4 121,939 + +0.7 121,984 - +0.2 122,169 + +0.3 122,184 + -0.5 122,345 + GFF3113 0.32 +1.2 122,346 - GFF3113 0.32 +0.1 122,476 - GFF3113 0.58 +0.2 122,476 - GFF3113 0.58 +2.0 123,376 + GFF3115 0.47 -0.9 123,376 + GFF3115 0.47 +0.2 123,376 + GFF3115 0.47 +0.1 123,376 + GFF3115 0.47 -2.3 123,376 + GFF3115 0.47 +0.4 123,376 + GFF3115 0.47 +0.1 123,377 - GFF3115 0.47 -0.9 123,377 - GFF3115 0.47 +1.7 123,515 + GFF3115 0.81 +1.3 123,515 + GFF3115 0.81 -1.4 123,516 - GFF3115 0.81 -1.3 123,602 + -0.5 123,606 + +0.3
Or see this region's nucleotide sequence