Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3091 and GFF3092 are separated by 395 nucleotides GFF3092 and GFF3093 are separated by 404 nucleotides
GFF3091 - Possible hydrolase, at 98,280 to 99,158
GFF3091
GFF3092 - Hexuronate transporter, at 99,554 to 100,858
GFF3092
GFF3093 - Mannonate dehydratase (EC 4.2.1.8), at 101,263 to 102,447
GFF3093
Position (kb) 99
100
101 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 98.613 kb on - strand, within GFF3091 at 98.703 kb on - strand, within GFF3091 at 98.744 kb on + strand, within GFF3091 at 98.761 kb on + strand, within GFF3091 at 98.786 kb on + strand, within GFF3091 at 98.795 kb on + strand, within GFF3091 at 98.807 kb on - strand, within GFF3091 at 98.831 kb on + strand, within GFF3091 at 98.832 kb on - strand, within GFF3091 at 98.832 kb on - strand, within GFF3091 at 98.832 kb on - strand, within GFF3091 at 98.832 kb on - strand, within GFF3091 at 98.934 kb on - strand, within GFF3091 at 99.620 kb on + strand at 99.851 kb on - strand, within GFF3092 at 99.851 kb on - strand, within GFF3092 at 99.886 kb on + strand, within GFF3092 at 99.931 kb on + strand, within GFF3092 at 99.937 kb on + strand, within GFF3092 at 100.221 kb on + strand, within GFF3092 at 100.221 kb on + strand, within GFF3092 at 100.293 kb on + strand, within GFF3092 at 100.373 kb on - strand, within GFF3092 at 100.566 kb on - strand, within GFF3092 at 100.625 kb on - strand, within GFF3092 at 100.663 kb on - strand, within GFF3092 at 100.775 kb on - strand at 100.794 kb on + strand at 100.795 kb on - strand at 100.861 kb on + strand at 100.996 kb on + strand at 101.263 kb on - strand at 101.292 kb on + strand at 101.456 kb on + strand, within GFF3093 at 101.456 kb on + strand, within GFF3093 at 101.456 kb on + strand, within GFF3093 at 101.456 kb on + strand, within GFF3093 at 101.457 kb on - strand, within GFF3093 at 101.532 kb on + strand, within GFF3093 at 101.533 kb on - strand, within GFF3093 at 101.551 kb on - strand, within GFF3093 at 101.555 kb on - strand, within GFF3093 at 101.556 kb on + strand, within GFF3093 at 101.568 kb on + strand, within GFF3093 at 101.574 kb on + strand, within GFF3093 at 101.599 kb on - strand, within GFF3093 at 101.599 kb on - strand, within GFF3093 at 101.611 kb on + strand, within GFF3093 at 101.611 kb on + strand, within GFF3093 at 101.611 kb on + strand, within GFF3093 at 101.611 kb on + strand, within GFF3093 at 101.615 kb on + strand, within GFF3093 at 101.616 kb on - strand, within GFF3093 at 101.616 kb on - strand, within GFF3093 at 101.616 kb on - strand, within GFF3093 at 101.710 kb on + strand, within GFF3093 at 101.713 kb on - strand, within GFF3093 at 101.824 kb on + strand, within GFF3093 at 101.827 kb on - strand, within GFF3093 at 101.827 kb on - strand, within GFF3093
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 98,613 - GFF3091 0.38 +2.0 98,703 - GFF3091 0.48 -0.0 98,744 + GFF3091 0.53 -0.8 98,761 + GFF3091 0.55 +1.0 98,786 + GFF3091 0.58 -0.3 98,795 + GFF3091 0.59 -1.5 98,807 - GFF3091 0.60 -0.2 98,831 + GFF3091 0.63 +0.1 98,832 - GFF3091 0.63 -1.5 98,832 - GFF3091 0.63 -0.9 98,832 - GFF3091 0.63 +0.8 98,832 - GFF3091 0.63 -0.6 98,934 - GFF3091 0.74 +0.4 99,620 + +0.2 99,851 - GFF3092 0.23 +0.8 99,851 - GFF3092 0.23 -0.1 99,886 + GFF3092 0.25 -3.3 99,931 + GFF3092 0.29 -1.0 99,937 + GFF3092 0.29 +1.3 100,221 + GFF3092 0.51 -0.3 100,221 + GFF3092 0.51 +0.9 100,293 + GFF3092 0.57 +0.5 100,373 - GFF3092 0.63 +0.8 100,566 - GFF3092 0.78 -0.3 100,625 - GFF3092 0.82 +1.4 100,663 - GFF3092 0.85 +0.6 100,775 - +0.5 100,794 + -0.3 100,795 - -0.5 100,861 + +1.6 100,996 + -0.8 101,263 - -0.1 101,292 + +0.3 101,456 + GFF3093 0.16 -1.1 101,456 + GFF3093 0.16 +0.0 101,456 + GFF3093 0.16 +1.3 101,456 + GFF3093 0.16 +0.9 101,457 - GFF3093 0.16 -0.1 101,532 + GFF3093 0.23 -1.5 101,533 - GFF3093 0.23 -0.7 101,551 - GFF3093 0.24 +0.4 101,555 - GFF3093 0.25 +1.0 101,556 + GFF3093 0.25 -2.5 101,568 + GFF3093 0.26 +1.8 101,574 + GFF3093 0.26 +1.0 101,599 - GFF3093 0.28 -0.5 101,599 - GFF3093 0.28 -1.4 101,611 + GFF3093 0.29 -0.4 101,611 + GFF3093 0.29 -0.5 101,611 + GFF3093 0.29 -0.7 101,611 + GFF3093 0.29 +0.5 101,615 + GFF3093 0.30 -0.1 101,616 - GFF3093 0.30 +0.1 101,616 - GFF3093 0.30 +1.9 101,616 - GFF3093 0.30 +0.6 101,710 + GFF3093 0.38 +1.0 101,713 - GFF3093 0.38 +0.5 101,824 + GFF3093 0.47 +0.9 101,827 - GFF3093 0.48 -1.3 101,827 - GFF3093 0.48 -0.5
Or see this region's nucleotide sequence