Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2979 and GFF2980 overlap by 4 nucleotides GFF2980 and GFF2981 are separated by 549 nucleotides GFF2981 and GFF2982 overlap by 45 nucleotides GFF2982 and GFF2983 are separated by 18 nucleotides GFF2983 and GFF3213 are separated by 131 nucleotides
GFF2979 - VapC toxin protein, at 290 to 691
GFF2979
GFF2980 - VapB protein (antitoxin to VapC), at 688 to 870
GFF2980
GFF2981 - FIG01047567: hypothetical protein, at 1,420 to 1,533
GFF2981
GFF2982 - hypothetical protein, at 1,489 to 1,605
GFF2982
GFF2983 - probable lipoprotein YPO2292, at 1,624 to 2,424
GFF2983
GFF3213 - tRNA-Gly-CCC, at 2,556 to 2,629
GFF3213
Position (kb)
1
2 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 0.500 kb on - strand, within GFF2979 at 0.500 kb on - strand, within GFF2979 at 0.523 kb on + strand, within GFF2979 at 0.555 kb on + strand, within GFF2979 at 0.602 kb on - strand, within GFF2979 at 0.602 kb on - strand, within GFF2979 at 0.603 kb on - strand, within GFF2979 at 0.656 kb on - strand at 0.662 kb on - strand at 0.662 kb on - strand at 0.915 kb on + strand at 0.972 kb on - strand at 0.972 kb on - strand at 1.229 kb on + strand at 1.229 kb on + strand at 1.234 kb on + strand at 1.235 kb on - strand at 1.410 kb on - strand at 1.423 kb on + strand at 1.423 kb on + strand at 1.423 kb on + strand at 1.424 kb on - strand at 1.538 kb on + strand, within GFF2982 at 1.623 kb on - strand at 1.623 kb on - strand at 1.768 kb on + strand, within GFF2983 at 1.921 kb on + strand, within GFF2983 at 1.922 kb on - strand, within GFF2983 at 1.969 kb on - strand, within GFF2983 at 1.969 kb on - strand, within GFF2983 at 2.072 kb on + strand, within GFF2983 at 2.173 kb on - strand, within GFF2983 at 2.239 kb on + strand, within GFF2983 at 2.239 kb on + strand, within GFF2983 at 2.239 kb on + strand, within GFF2983 at 2.295 kb on - strand, within GFF2983 at 2.470 kb on - strand at 2.470 kb on - strand at 2.585 kb on + strand, within GFF3213 at 2.588 kb on - strand, within GFF3213
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 500 - GFF2979 0.52 +0.3 500 - GFF2979 0.52 +0.3 523 + GFF2979 0.58 +0.9 555 + GFF2979 0.66 -0.7 602 - GFF2979 0.78 +0.3 602 - GFF2979 0.78 +0.8 603 - GFF2979 0.78 +0.3 656 - -0.3 662 - +0.3 662 - -0.0 915 + +0.1 972 - +0.4 972 - -0.0 1,229 + +0.1 1,229 + +1.2 1,234 + -1.0 1,235 - +1.0 1,410 - +0.2 1,423 + +0.1 1,423 + +1.1 1,423 + +2.0 1,424 - -0.1 1,538 + GFF2982 0.42 +1.8 1,623 - -0.6 1,623 - +1.9 1,768 + GFF2983 0.18 +0.4 1,921 + GFF2983 0.37 -0.7 1,922 - GFF2983 0.37 +0.5 1,969 - GFF2983 0.43 +0.7 1,969 - GFF2983 0.43 +0.7 2,072 + GFF2983 0.56 +1.3 2,173 - GFF2983 0.69 +0.3 2,239 + GFF2983 0.77 +0.2 2,239 + GFF2983 0.77 -3.2 2,239 + GFF2983 0.77 +1.1 2,295 - GFF2983 0.84 -0.1 2,470 - +1.2 2,470 - +1.3 2,585 + GFF3213 0.39 +1.2 2,588 - GFF3213 0.43 +0.1
Or see this region's nucleotide sequence