Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2939 and GFF2940 are separated by 212 nucleotides GFF2940 and GFF2941 are separated by 13 nucleotides
GFF2939 - Uncharacterized ABC transporter, ATP-binding protein YrbF, at 20,422 to 21,234
GFF2939
GFF2940 - Inner membrane protein YrbG, predicted calcium/sodium:proton antiporter, at 21,447 to 22,424
GFF2940
GFF2941 - Arabinose 5-phosphate isomerase (EC 5.3.1.13), at 22,438 to 23,424
GFF2941
Position (kb) 21
22
23 Strain fitness (log2 ratio) -2
-1
0
1
2 at 20.605 kb on - strand, within GFF2939 at 20.700 kb on + strand, within GFF2939 at 20.873 kb on - strand, within GFF2939 at 20.913 kb on - strand, within GFF2939 at 20.976 kb on + strand, within GFF2939 at 20.976 kb on + strand, within GFF2939 at 20.977 kb on - strand, within GFF2939 at 21.037 kb on + strand, within GFF2939 at 21.038 kb on - strand, within GFF2939 at 21.097 kb on - strand, within GFF2939 at 21.156 kb on + strand at 21.250 kb on - strand at 21.250 kb on - strand at 21.300 kb on + strand at 21.301 kb on - strand at 21.304 kb on + strand at 21.304 kb on + strand at 21.311 kb on + strand at 21.401 kb on - strand at 21.401 kb on - strand at 21.424 kb on + strand at 21.426 kb on + strand at 21.539 kb on + strand at 21.539 kb on + strand at 21.674 kb on + strand, within GFF2940 at 21.674 kb on + strand, within GFF2940 at 21.674 kb on + strand, within GFF2940 at 21.675 kb on - strand, within GFF2940 at 21.686 kb on + strand, within GFF2940 at 21.700 kb on + strand, within GFF2940 at 21.748 kb on + strand, within GFF2940 at 21.748 kb on + strand, within GFF2940 at 21.749 kb on - strand, within GFF2940 at 21.772 kb on + strand, within GFF2940 at 21.772 kb on + strand, within GFF2940 at 21.833 kb on + strand, within GFF2940 at 21.834 kb on - strand, within GFF2940 at 21.859 kb on + strand, within GFF2940 at 21.860 kb on - strand, within GFF2940 at 21.868 kb on + strand, within GFF2940 at 22.053 kb on + strand, within GFF2940 at 22.053 kb on + strand, within GFF2940 at 22.273 kb on - strand, within GFF2940 at 22.273 kb on - strand, within GFF2940 at 22.273 kb on - strand, within GFF2940 at 22.352 kb on + strand at 22.353 kb on - strand at 22.379 kb on - strand at 22.534 kb on + strand at 22.536 kb on + strand at 22.878 kb on + strand, within GFF2941 at 22.926 kb on + strand, within GFF2941 at 22.926 kb on + strand, within GFF2941 at 22.973 kb on + strand, within GFF2941 at 23.042 kb on + strand, within GFF2941 at 23.391 kb on + strand at 23.391 kb on + strand at 23.391 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 20,605 - GFF2939 0.23 +0.4 20,700 + GFF2939 0.34 -0.8 20,873 - GFF2939 0.55 +0.3 20,913 - GFF2939 0.60 +2.2 20,976 + GFF2939 0.68 +0.7 20,976 + GFF2939 0.68 +0.4 20,977 - GFF2939 0.68 -0.5 21,037 + GFF2939 0.76 -0.7 21,038 - GFF2939 0.76 -0.3 21,097 - GFF2939 0.83 +0.7 21,156 + -0.2 21,250 - +0.7 21,250 - -0.0 21,300 + +1.2 21,301 - -0.5 21,304 + +0.8 21,304 + -0.4 21,311 + +0.9 21,401 - -0.8 21,401 - -2.1 21,424 + +1.5 21,426 + +0.7 21,539 + +1.3 21,539 + +0.1 21,674 + GFF2940 0.23 -2.1 21,674 + GFF2940 0.23 +0.2 21,674 + GFF2940 0.23 +0.5 21,675 - GFF2940 0.23 -2.5 21,686 + GFF2940 0.24 +1.3 21,700 + GFF2940 0.26 -0.2 21,748 + GFF2940 0.31 -0.8 21,748 + GFF2940 0.31 -2.0 21,749 - GFF2940 0.31 +0.3 21,772 + GFF2940 0.33 -1.0 21,772 + GFF2940 0.33 -1.6 21,833 + GFF2940 0.39 +0.9 21,834 - GFF2940 0.40 -0.3 21,859 + GFF2940 0.42 +0.9 21,860 - GFF2940 0.42 +0.8 21,868 + GFF2940 0.43 +0.1 22,053 + GFF2940 0.62 +0.4 22,053 + GFF2940 0.62 +0.4 22,273 - GFF2940 0.84 +0.2 22,273 - GFF2940 0.84 +0.7 22,273 - GFF2940 0.84 -0.1 22,352 + -1.2 22,353 - +0.4 22,379 - -0.8 22,534 + -0.0 22,536 + +0.9 22,878 + GFF2941 0.45 +1.0 22,926 + GFF2941 0.49 +1.5 22,926 + GFF2941 0.49 -0.1 22,973 + GFF2941 0.54 -1.9 23,042 + GFF2941 0.61 +0.3 23,391 + -1.9 23,391 + +1.2 23,391 + +0.6
Or see this region's nucleotide sequence