Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2933 and GFF2934 are separated by 53 nucleotides GFF2934 and GFF2935 are separated by 187 nucleotides GFF2935 and GFF2936 overlap by 1 nucleotides GFF2936 and GFF2937 are separated by 18 nucleotides GFF2937 and GFF2938 are separated by 4 nucleotides
GFF2933 - UDP-N-acetylglucosamine 1-carboxyvinyltransferase (EC 2.5.1.7), at 16,371 to 17,630
GFF2933
GFF2934 - YrbA protein, at 17,684 to 17,938
GFF2934
GFF2935 - Uncharacterized protein YrbB, at 18,126 to 18,422
GFF2935
GFF2936 - Uncharacterized ABC transporter, auxiliary component YrbC, at 18,422 to 19,057
GFF2936
GFF2937 - Uncharacterized ABC transporter, periplasmic component YrbD, at 19,076 to 19,627
GFF2937
GFF2938 - Uncharacterized ABC transporter, permease component YrbE, at 19,632 to 20,414
GFF2938
Position (kb) 18
19
20 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 17.686 kb on - strand at 17.729 kb on - strand, within GFF2934 at 17.729 kb on - strand, within GFF2934 at 17.731 kb on - strand, within GFF2934 at 17.756 kb on - strand, within GFF2934 at 18.026 kb on - strand at 18.079 kb on + strand at 18.100 kb on - strand at 18.100 kb on - strand at 18.127 kb on + strand at 18.127 kb on + strand at 18.128 kb on - strand at 18.159 kb on - strand, within GFF2935 at 18.160 kb on + strand, within GFF2935 at 18.160 kb on + strand, within GFF2935 at 18.163 kb on - strand, within GFF2935 at 18.163 kb on - strand, within GFF2935 at 18.163 kb on - strand, within GFF2935 at 18.163 kb on - strand, within GFF2935 at 18.273 kb on + strand, within GFF2935 at 18.273 kb on + strand, within GFF2935 at 18.423 kb on + strand at 18.461 kb on - strand at 18.461 kb on - strand at 18.568 kb on - strand, within GFF2936 at 18.724 kb on - strand, within GFF2936 at 18.724 kb on - strand, within GFF2936 at 18.814 kb on - strand, within GFF2936 at 18.844 kb on - strand, within GFF2936 at 19.011 kb on - strand at 19.078 kb on - strand at 19.173 kb on + strand, within GFF2937 at 19.192 kb on + strand, within GFF2937 at 19.352 kb on - strand, within GFF2937 at 19.357 kb on + strand, within GFF2937 at 19.359 kb on + strand, within GFF2937 at 19.360 kb on - strand, within GFF2937 at 19.418 kb on - strand, within GFF2937 at 19.529 kb on - strand, within GFF2937 at 19.751 kb on - strand, within GFF2938 at 19.827 kb on - strand, within GFF2938 at 19.934 kb on + strand, within GFF2938 at 19.934 kb on + strand, within GFF2938 at 19.944 kb on + strand, within GFF2938
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 17,686 - -0.7 17,729 - GFF2934 0.18 -1.3 17,729 - GFF2934 0.18 -0.2 17,731 - GFF2934 0.18 -2.4 17,756 - GFF2934 0.28 +0.3 18,026 - +0.3 18,079 + -0.1 18,100 - +0.2 18,100 - +0.2 18,127 + +0.1 18,127 + -1.3 18,128 - +0.6 18,159 - GFF2935 0.11 +0.1 18,160 + GFF2935 0.11 +1.0 18,160 + GFF2935 0.11 +0.9 18,163 - GFF2935 0.12 -0.4 18,163 - GFF2935 0.12 -0.6 18,163 - GFF2935 0.12 +1.0 18,163 - GFF2935 0.12 -1.8 18,273 + GFF2935 0.49 +0.2 18,273 + GFF2935 0.49 +1.2 18,423 + -0.2 18,461 - -2.1 18,461 - +0.5 18,568 - GFF2936 0.23 +0.4 18,724 - GFF2936 0.47 -0.9 18,724 - GFF2936 0.47 +0.5 18,814 - GFF2936 0.62 -2.3 18,844 - GFF2936 0.66 -0.6 19,011 - +0.6 19,078 - +0.4 19,173 + GFF2937 0.18 -2.3 19,192 + GFF2937 0.21 +1.0 19,352 - GFF2937 0.50 -0.3 19,357 + GFF2937 0.51 +0.5 19,359 + GFF2937 0.51 -0.9 19,360 - GFF2937 0.51 -2.1 19,418 - GFF2937 0.62 +0.6 19,529 - GFF2937 0.82 -0.7 19,751 - GFF2938 0.15 -3.1 19,827 - GFF2938 0.25 +0.6 19,934 + GFF2938 0.39 +1.0 19,934 + GFF2938 0.39 +0.7 19,944 + GFF2938 0.40 +0.3
Or see this region's nucleotide sequence