Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2905 and GFF2906 are separated by 24 nucleotides GFF2906 and GFF2907 are separated by 118 nucleotides GFF2907 and GFF2910 are separated by 101 nucleotides GFF2910 and GFF2911 are separated by 8 nucleotides GFF2911 and GFF2912 are separated by 52 nucleotides
GFF2905 - MG(2+) CHELATASE FAMILY PROTEIN / ComM-related protein, at 32,807 to 34,327
GFF2905
GFF2906 - Protein yifE, at 34,352 to 34,690
GFF2906
GFF2907 - HTH-type transcriptional regulator hdfR, at 34,809 to 35,645
GFF2907
GFF2910 - tRNA-Trp-CCA, at 35,747 to 35,822
GFF2910
GFF2911 - tRNA-Asp-GTC, at 35,831 to 35,907
GFF2911
GFF2912 - 5S RNA, at 35,960 to 36,079
GFF2912
Position (kb) 34
35
36 Strain fitness (log2 ratio) -1
0
1
2
3 at 33.825 kb on + strand, within GFF2905 at 33.900 kb on + strand, within GFF2905 at 33.900 kb on + strand, within GFF2905 at 33.900 kb on + strand, within GFF2905 at 33.900 kb on + strand, within GFF2905 at 33.900 kb on + strand, within GFF2905 at 33.901 kb on - strand, within GFF2905 at 34.059 kb on - strand, within GFF2905 at 34.113 kb on - strand, within GFF2905 at 34.197 kb on + strand at 34.228 kb on - strand at 34.273 kb on - strand at 34.351 kb on - strand at 34.372 kb on - strand at 34.448 kb on - strand, within GFF2906 at 34.449 kb on + strand, within GFF2906 at 34.449 kb on + strand, within GFF2906 at 34.450 kb on - strand, within GFF2906 at 34.502 kb on + strand, within GFF2906 at 34.642 kb on - strand, within GFF2906 at 34.704 kb on - strand at 34.739 kb on + strand at 34.884 kb on + strand at 34.884 kb on + strand at 34.914 kb on - strand, within GFF2907 at 34.970 kb on + strand, within GFF2907 at 35.028 kb on - strand, within GFF2907 at 35.114 kb on + strand, within GFF2907 at 35.114 kb on + strand, within GFF2907 at 35.143 kb on - strand, within GFF2907 at 35.150 kb on - strand, within GFF2907 at 35.290 kb on + strand, within GFF2907 at 35.311 kb on + strand, within GFF2907 at 35.314 kb on - strand, within GFF2907 at 35.314 kb on - strand, within GFF2907 at 35.483 kb on + strand, within GFF2907 at 35.528 kb on + strand, within GFF2907 at 35.564 kb on - strand at 35.565 kb on + strand at 35.565 kb on + strand at 35.591 kb on + strand at 35.674 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 33,825 + GFF2905 0.67 +0.5 33,900 + GFF2905 0.72 +0.9 33,900 + GFF2905 0.72 +0.8 33,900 + GFF2905 0.72 -0.3 33,900 + GFF2905 0.72 +0.1 33,900 + GFF2905 0.72 -0.8 33,901 - GFF2905 0.72 +0.3 34,059 - GFF2905 0.82 +1.1 34,113 - GFF2905 0.86 +0.2 34,197 + +0.5 34,228 - -0.8 34,273 - +0.1 34,351 - +0.2 34,372 - +0.3 34,448 - GFF2906 0.28 -1.1 34,449 + GFF2906 0.29 -0.5 34,449 + GFF2906 0.29 +0.7 34,450 - GFF2906 0.29 -0.2 34,502 + GFF2906 0.44 +1.7 34,642 - GFF2906 0.86 -1.4 34,704 - +0.4 34,739 + +0.7 34,884 + +0.9 34,884 + -1.5 34,914 - GFF2907 0.13 +0.4 34,970 + GFF2907 0.19 -0.9 35,028 - GFF2907 0.26 -1.6 35,114 + GFF2907 0.36 +1.2 35,114 + GFF2907 0.36 +3.0 35,143 - GFF2907 0.40 +2.6 35,150 - GFF2907 0.41 +2.4 35,290 + GFF2907 0.57 +0.4 35,311 + GFF2907 0.60 -0.2 35,314 - GFF2907 0.60 -0.3 35,314 - GFF2907 0.60 +0.9 35,483 + GFF2907 0.81 +1.2 35,528 + GFF2907 0.86 +1.3 35,564 - +3.0 35,565 + -1.7 35,565 + +0.1 35,591 + +0.4 35,674 + -0.1
Or see this region's nucleotide sequence