Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2743 and GFF2744 are separated by 11 nucleotides GFF2744 and GFF2745 are separated by 14 nucleotides GFF2745 and GFF2746 are separated by 121 nucleotides
GFF2743 - Oligopeptide transport ATP-binding protein OppD (TC 3.A.1.5.1), at 262,242 to 263,249
GFF2743
GFF2744 - Oligopeptide transport system permease protein OppC (TC 3.A.1.5.1), at 263,261 to 264,169
GFF2744
GFF2745 - Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1), at 264,184 to 265,104
GFF2745
GFF2746 - Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein OppA (TC 3.A.1.5.1), at 265,226 to 266,857
GFF2746
Position (kb) 264
265
266 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 263.305 kb on + strand at 263.496 kb on + strand, within GFF2744 at 263.806 kb on - strand, within GFF2744 at 263.856 kb on - strand, within GFF2744 at 264.024 kb on - strand, within GFF2744 at 264.041 kb on - strand, within GFF2744 at 264.161 kb on + strand at 264.162 kb on - strand at 264.162 kb on - strand at 264.216 kb on - strand at 264.242 kb on - strand at 264.242 kb on - strand at 264.278 kb on - strand, within GFF2745 at 264.402 kb on - strand, within GFF2745 at 264.532 kb on - strand, within GFF2745 at 264.549 kb on + strand, within GFF2745 at 264.718 kb on - strand, within GFF2745 at 264.948 kb on - strand, within GFF2745 at 265.131 kb on + strand at 265.330 kb on - strand at 265.330 kb on - strand at 265.357 kb on + strand at 265.457 kb on - strand, within GFF2746 at 265.457 kb on - strand, within GFF2746 at 265.520 kb on - strand, within GFF2746 at 265.522 kb on - strand, within GFF2746 at 265.760 kb on - strand, within GFF2746 at 265.868 kb on - strand, within GFF2746 at 266.039 kb on - strand, within GFF2746 at 266.045 kb on - strand, within GFF2746
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 263,305 + -1.5 263,496 + GFF2744 0.26 -1.4 263,806 - GFF2744 0.60 +1.3 263,856 - GFF2744 0.65 -0.3 264,024 - GFF2744 0.84 +0.2 264,041 - GFF2744 0.86 -0.2 264,161 + +0.8 264,162 - +0.2 264,162 - -0.1 264,216 - -0.3 264,242 - +1.4 264,242 - -1.2 264,278 - GFF2745 0.10 +0.5 264,402 - GFF2745 0.24 +0.3 264,532 - GFF2745 0.38 +3.0 264,549 + GFF2745 0.40 +0.9 264,718 - GFF2745 0.58 -1.2 264,948 - GFF2745 0.83 +0.9 265,131 + -1.5 265,330 - +0.2 265,330 - -0.7 265,357 + +0.4 265,457 - GFF2746 0.14 -0.1 265,457 - GFF2746 0.14 +0.7 265,520 - GFF2746 0.18 -2.3 265,522 - GFF2746 0.18 +0.3 265,760 - GFF2746 0.33 +0.6 265,868 - GFF2746 0.39 -0.4 266,039 - GFF2746 0.50 +0.6 266,045 - GFF2746 0.50 +0.3
Or see this region's nucleotide sequence