Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2639 and GFF2640 are separated by 43 nucleotides GFF2640 and GFF2641 are separated by 38 nucleotides GFF2641 and GFF2642 are separated by 105 nucleotides GFF2642 and GFF2643 are separated by 58 nucleotides GFF2643 and GFF2644 are separated by 213 nucleotides GFF2644 and GFF2645 are separated by 49 nucleotides
GFF2639 - Heat shock protein hslJ, at 166,747 to 167,157
GFF2639
GFF2640 - probable lipoprotein YPO2331, at 167,201 to 167,467
GFF2640
GFF2641 - hypothetical protein, at 167,506 to 167,637
GFF2641
GFF2642 - Pyruvate-flavodoxin oxidoreductase (EC 1.2.7.-), at 167,743 to 171,267
GFF2642
GFF2643 - FIG01048335: hypothetical protein, at 171,326 to 171,535
GFF2643
GFF2644 - Universal stress protein F, at 171,749 to 172,183
GFF2644
GFF2645 - Ethidium bromide-methyl viologen resistance protein EmrE, at 172,233 to 172,571
GFF2645
Position (kb) 167
168
169
170
171
172 Strain fitness (log2 ratio) -2
-1
0
1
2 at 167.029 kb on - strand, within GFF2639 at 167.131 kb on + strand at 167.131 kb on + strand at 167.177 kb on + strand at 167.429 kb on + strand, within GFF2640 at 167.430 kb on - strand, within GFF2640 at 167.508 kb on - strand at 167.512 kb on - strand at 167.575 kb on + strand, within GFF2641 at 167.731 kb on + strand at 167.731 kb on + strand at 167.742 kb on + strand at 167.875 kb on - strand at 168.023 kb on - strand at 168.023 kb on - strand at 168.024 kb on + strand at 168.054 kb on - strand at 168.281 kb on + strand, within GFF2642 at 168.404 kb on - strand, within GFF2642 at 168.437 kb on - strand, within GFF2642 at 168.437 kb on - strand, within GFF2642 at 168.503 kb on - strand, within GFF2642 at 168.552 kb on - strand, within GFF2642 at 168.600 kb on - strand, within GFF2642 at 168.839 kb on + strand, within GFF2642 at 169.181 kb on + strand, within GFF2642 at 169.217 kb on + strand, within GFF2642 at 169.235 kb on - strand, within GFF2642 at 169.281 kb on + strand, within GFF2642 at 169.510 kb on + strand, within GFF2642 at 169.512 kb on + strand, within GFF2642 at 169.810 kb on - strand, within GFF2642 at 169.818 kb on + strand, within GFF2642 at 169.977 kb on + strand, within GFF2642 at 169.978 kb on - strand, within GFF2642 at 169.978 kb on - strand, within GFF2642 at 169.997 kb on + strand, within GFF2642 at 170.226 kb on + strand, within GFF2642 at 170.243 kb on + strand, within GFF2642 at 170.245 kb on + strand, within GFF2642 at 170.245 kb on + strand, within GFF2642 at 170.323 kb on - strand, within GFF2642 at 170.641 kb on + strand, within GFF2642 at 170.641 kb on + strand, within GFF2642 at 170.642 kb on - strand, within GFF2642 at 170.642 kb on - strand, within GFF2642 at 170.697 kb on + strand, within GFF2642 at 170.698 kb on - strand, within GFF2642 at 170.836 kb on + strand, within GFF2642 at 170.836 kb on + strand, within GFF2642 at 170.836 kb on + strand, within GFF2642 at 170.837 kb on - strand, within GFF2642 at 170.848 kb on + strand, within GFF2642 at 170.849 kb on - strand, within GFF2642 at 170.944 kb on + strand at 170.944 kb on + strand at 170.946 kb on + strand at 170.946 kb on + strand at 171.032 kb on - strand at 171.402 kb on - strand, within GFF2643 at 171.569 kb on - strand at 171.594 kb on + strand at 171.598 kb on + strand at 171.616 kb on + strand at 171.616 kb on + strand at 171.619 kb on + strand at 171.684 kb on - strand at 171.908 kb on + strand, within GFF2644 at 172.108 kb on + strand, within GFF2644 at 172.112 kb on - strand, within GFF2644 at 172.116 kb on - strand, within GFF2644 at 172.116 kb on - strand, within GFF2644 at 172.182 kb on - strand at 172.267 kb on - strand, within GFF2645
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 167,029 - GFF2639 0.69 -0.1 167,131 + -1.0 167,131 + +0.2 167,177 + -0.1 167,429 + GFF2640 0.85 +0.6 167,430 - GFF2640 0.86 -0.9 167,508 - +0.8 167,512 - -0.7 167,575 + GFF2641 0.52 -0.4 167,731 + -0.5 167,731 + +0.6 167,742 + +0.7 167,875 - -1.9 168,023 - +0.9 168,023 - +0.4 168,024 + +0.8 168,054 - -0.7 168,281 + GFF2642 0.15 -0.2 168,404 - GFF2642 0.19 -1.0 168,437 - GFF2642 0.20 +0.2 168,437 - GFF2642 0.20 +0.1 168,503 - GFF2642 0.22 +0.7 168,552 - GFF2642 0.23 -1.6 168,600 - GFF2642 0.24 -0.6 168,839 + GFF2642 0.31 -0.7 169,181 + GFF2642 0.41 -1.1 169,217 + GFF2642 0.42 +0.6 169,235 - GFF2642 0.42 +0.4 169,281 + GFF2642 0.44 -0.2 169,510 + GFF2642 0.50 +0.2 169,512 + GFF2642 0.50 -0.3 169,810 - GFF2642 0.59 -0.4 169,818 + GFF2642 0.59 +0.7 169,977 + GFF2642 0.63 +0.7 169,978 - GFF2642 0.63 +0.0 169,978 - GFF2642 0.63 +0.9 169,997 + GFF2642 0.64 -0.0 170,226 + GFF2642 0.70 -0.5 170,243 + GFF2642 0.71 +0.5 170,245 + GFF2642 0.71 -0.2 170,245 + GFF2642 0.71 +0.1 170,323 - GFF2642 0.73 +0.7 170,641 + GFF2642 0.82 -1.1 170,641 + GFF2642 0.82 -0.8 170,642 - GFF2642 0.82 -0.6 170,642 - GFF2642 0.82 +0.2 170,697 + GFF2642 0.84 +0.5 170,698 - GFF2642 0.84 +0.8 170,836 + GFF2642 0.88 +0.6 170,836 + GFF2642 0.88 -0.0 170,836 + GFF2642 0.88 -0.1 170,837 - GFF2642 0.88 +0.3 170,848 + GFF2642 0.88 +0.4 170,849 - GFF2642 0.88 +2.3 170,944 + -1.1 170,944 + -0.7 170,946 + +0.5 170,946 + -0.5 171,032 - +0.8 171,402 - GFF2643 0.36 -0.7 171,569 - -0.4 171,594 + -0.0 171,598 + +0.2 171,616 + +0.1 171,616 + -0.6 171,619 + +0.9 171,684 - -0.1 171,908 + GFF2644 0.37 +1.7 172,108 + GFF2644 0.83 -1.3 172,112 - GFF2644 0.83 +0.5 172,116 - GFF2644 0.84 -0.6 172,116 - GFF2644 0.84 +0.3 172,182 - +0.7 172,267 - GFF2645 0.10 -0.4
Or see this region's nucleotide sequence