Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2329 and GFF2330 are separated by 164 nucleotides GFF2330 and GFF2331 are separated by 57 nucleotides GFF2331 and GFF2332 are separated by 258 nucleotides
GFF2329 - 5,10-methylenetetrahydrofolate reductase (EC 1.5.1.20), at 117,452 to 118,342
GFF2329
GFF2330 - Catalase (EC 1.11.1.6) / Peroxidase (EC 1.11.1.7), at 118,507 to 120,687
GFF2330
GFF2331 - Putative periplasmic protein, at 120,745 to 121,368
GFF2331
GFF2332 - Glycerol dehydrogenase (EC 1.1.1.6), at 121,627 to 122,730
GFF2332
Position (kb)
118
119
120
121 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 117.630 kb on - strand, within GFF2329 at 117.671 kb on - strand, within GFF2329 at 117.673 kb on + strand, within GFF2329 at 117.691 kb on + strand, within GFF2329 at 117.691 kb on + strand, within GFF2329 at 117.724 kb on + strand, within GFF2329 at 117.829 kb on + strand, within GFF2329 at 117.915 kb on + strand, within GFF2329 at 117.916 kb on - strand, within GFF2329 at 118.023 kb on - strand, within GFF2329 at 118.126 kb on + strand, within GFF2329 at 118.326 kb on + strand at 118.327 kb on - strand at 118.341 kb on - strand at 118.367 kb on + strand at 118.454 kb on + strand at 118.469 kb on + strand at 118.490 kb on + strand at 118.531 kb on - strand at 118.717 kb on + strand at 118.793 kb on - strand, within GFF2330 at 118.793 kb on - strand, within GFF2330 at 118.846 kb on - strand, within GFF2330 at 118.963 kb on + strand, within GFF2330 at 118.995 kb on + strand, within GFF2330 at 119.179 kb on - strand, within GFF2330 at 119.182 kb on + strand, within GFF2330 at 119.182 kb on + strand, within GFF2330 at 119.183 kb on - strand, within GFF2330 at 119.183 kb on - strand, within GFF2330 at 119.183 kb on - strand, within GFF2330 at 119.247 kb on + strand, within GFF2330 at 119.390 kb on + strand, within GFF2330 at 119.391 kb on - strand, within GFF2330 at 119.407 kb on + strand, within GFF2330 at 119.407 kb on + strand, within GFF2330 at 119.513 kb on - strand, within GFF2330 at 119.522 kb on + strand, within GFF2330 at 119.522 kb on + strand, within GFF2330 at 119.533 kb on - strand, within GFF2330 at 119.834 kb on - strand, within GFF2330 at 119.895 kb on + strand, within GFF2330 at 119.895 kb on + strand, within GFF2330 at 119.953 kb on - strand, within GFF2330 at 120.041 kb on + strand, within GFF2330 at 120.045 kb on - strand, within GFF2330 at 120.090 kb on + strand, within GFF2330 at 120.090 kb on + strand, within GFF2330 at 120.091 kb on - strand, within GFF2330 at 120.156 kb on - strand, within GFF2330 at 120.297 kb on + strand, within GFF2330 at 120.312 kb on - strand, within GFF2330 at 120.439 kb on - strand, within GFF2330 at 120.458 kb on - strand, within GFF2330 at 120.572 kb on + strand at 120.596 kb on - strand at 120.717 kb on - strand at 120.758 kb on + strand at 120.790 kb on + strand at 120.791 kb on - strand at 120.793 kb on + strand at 120.820 kb on + strand, within GFF2331 at 120.820 kb on + strand, within GFF2331 at 120.826 kb on - strand, within GFF2331 at 120.880 kb on - strand, within GFF2331 at 120.916 kb on - strand, within GFF2331 at 120.916 kb on - strand, within GFF2331 at 120.916 kb on - strand, within GFF2331 at 120.936 kb on + strand, within GFF2331 at 120.939 kb on - strand, within GFF2331 at 120.942 kb on + strand, within GFF2331 at 121.193 kb on + strand, within GFF2331 at 121.210 kb on + strand, within GFF2331 at 121.211 kb on - strand, within GFF2331 at 121.218 kb on + strand, within GFF2331 at 121.219 kb on - strand, within GFF2331 at 121.219 kb on - strand, within GFF2331 at 121.219 kb on - strand, within GFF2331 at 121.223 kb on - strand, within GFF2331 at 121.258 kb on - strand, within GFF2331 at 121.348 kb on - strand at 121.536 kb on + strand at 121.537 kb on - strand at 121.628 kb on + strand at 121.629 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 117,630 - GFF2329 0.20 +1.6 117,671 - GFF2329 0.25 -0.6 117,673 + GFF2329 0.25 -0.1 117,691 + GFF2329 0.27 -0.1 117,691 + GFF2329 0.27 -0.9 117,724 + GFF2329 0.31 -2.1 117,829 + GFF2329 0.42 -1.3 117,915 + GFF2329 0.52 +1.0 117,916 - GFF2329 0.52 +0.7 118,023 - GFF2329 0.64 -2.9 118,126 + GFF2329 0.76 +0.1 118,326 + -0.6 118,327 - +0.1 118,341 - -0.5 118,367 + +0.7 118,454 + +0.8 118,469 + +0.6 118,490 + +0.8 118,531 - -0.9 118,717 + +0.1 118,793 - GFF2330 0.13 +0.5 118,793 - GFF2330 0.13 +0.8 118,846 - GFF2330 0.16 -0.8 118,963 + GFF2330 0.21 +1.5 118,995 + GFF2330 0.22 -0.4 119,179 - GFF2330 0.31 +0.2 119,182 + GFF2330 0.31 +0.8 119,182 + GFF2330 0.31 +1.3 119,183 - GFF2330 0.31 +1.6 119,183 - GFF2330 0.31 +0.4 119,183 - GFF2330 0.31 -2.5 119,247 + GFF2330 0.34 -0.1 119,390 + GFF2330 0.40 +1.2 119,391 - GFF2330 0.41 +0.4 119,407 + GFF2330 0.41 +1.7 119,407 + GFF2330 0.41 +0.6 119,513 - GFF2330 0.46 +1.4 119,522 + GFF2330 0.47 -0.2 119,522 + GFF2330 0.47 -2.3 119,533 - GFF2330 0.47 -0.2 119,834 - GFF2330 0.61 -0.3 119,895 + GFF2330 0.64 -0.3 119,895 + GFF2330 0.64 +0.5 119,953 - GFF2330 0.66 +0.4 120,041 + GFF2330 0.70 +0.2 120,045 - GFF2330 0.71 +0.5 120,090 + GFF2330 0.73 +0.7 120,090 + GFF2330 0.73 +1.0 120,091 - GFF2330 0.73 -3.3 120,156 - GFF2330 0.76 -1.0 120,297 + GFF2330 0.82 +0.9 120,312 - GFF2330 0.83 +1.6 120,439 - GFF2330 0.89 -0.8 120,458 - GFF2330 0.89 -0.1 120,572 + -1.8 120,596 - -1.2 120,717 - -0.5 120,758 + -0.8 120,790 + -0.2 120,791 - -0.4 120,793 + -2.5 120,820 + GFF2331 0.12 -0.5 120,820 + GFF2331 0.12 -1.9 120,826 - GFF2331 0.13 -0.5 120,880 - GFF2331 0.22 -0.4 120,916 - GFF2331 0.27 +0.4 120,916 - GFF2331 0.27 -0.0 120,916 - GFF2331 0.27 +0.5 120,936 + GFF2331 0.31 -0.3 120,939 - GFF2331 0.31 +0.4 120,942 + GFF2331 0.32 +1.5 121,193 + GFF2331 0.72 +0.1 121,210 + GFF2331 0.75 +0.3 121,211 - GFF2331 0.75 +0.0 121,218 + GFF2331 0.76 +0.7 121,219 - GFF2331 0.76 +0.5 121,219 - GFF2331 0.76 +0.3 121,219 - GFF2331 0.76 +0.7 121,223 - GFF2331 0.77 -2.4 121,258 - GFF2331 0.82 -0.1 121,348 - +0.3 121,536 + +1.1 121,537 - +0.8 121,628 + -0.6 121,629 - +0.2
Or see this region's nucleotide sequence