Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2232 and GFF2233 are separated by 45 nucleotides GFF2233 and GFF2234 are separated by 43 nucleotides GFF2234 and GFF2235 are separated by 18 nucleotides
GFF2232 - Glucuronide transport protein YihP, homologous to YihO, at 26,161 to 27,543
GFF2232
GFF2233 - Alpha-glucosyltransferase YihQ, at 27,589 to 29,625
GFF2233
GFF2234 - Sugar-1-epimerase YihR, at 29,669 to 30,511
GFF2234
GFF2235 - Aldose-ketose isomerase YihS, at 30,530 to 31,771
GFF2235
Position (kb)
27
28
29
30 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 26.603 kb on - strand, within GFF2232 at 26.735 kb on + strand, within GFF2232 at 26.744 kb on + strand, within GFF2232 at 26.747 kb on + strand, within GFF2232 at 26.788 kb on - strand, within GFF2232 at 26.811 kb on + strand, within GFF2232 at 26.855 kb on - strand, within GFF2232 at 26.855 kb on - strand, within GFF2232 at 26.931 kb on + strand, within GFF2232 at 26.936 kb on - strand, within GFF2232 at 26.975 kb on + strand, within GFF2232 at 27.030 kb on + strand, within GFF2232 at 27.169 kb on - strand, within GFF2232 at 27.169 kb on - strand, within GFF2232 at 27.169 kb on - strand, within GFF2232 at 27.228 kb on + strand, within GFF2232 at 27.342 kb on + strand, within GFF2232 at 27.459 kb on + strand at 27.466 kb on + strand at 27.591 kb on - strand at 27.714 kb on + strand at 27.714 kb on + strand at 27.715 kb on - strand at 27.807 kb on - strand, within GFF2233 at 27.813 kb on - strand, within GFF2233 at 27.824 kb on + strand, within GFF2233 at 27.930 kb on + strand, within GFF2233 at 27.964 kb on - strand, within GFF2233 at 28.313 kb on + strand, within GFF2233 at 28.314 kb on - strand, within GFF2233 at 28.314 kb on - strand, within GFF2233 at 28.538 kb on + strand, within GFF2233 at 28.617 kb on - strand, within GFF2233 at 28.626 kb on + strand, within GFF2233 at 28.628 kb on + strand, within GFF2233 at 28.628 kb on + strand, within GFF2233 at 28.629 kb on - strand, within GFF2233 at 28.689 kb on - strand, within GFF2233 at 28.773 kb on + strand, within GFF2233 at 28.897 kb on + strand, within GFF2233 at 28.898 kb on - strand, within GFF2233 at 28.932 kb on - strand, within GFF2233 at 28.932 kb on - strand, within GFF2233 at 28.955 kb on - strand, within GFF2233 at 28.955 kb on - strand, within GFF2233 at 28.965 kb on + strand, within GFF2233 at 29.021 kb on + strand, within GFF2233 at 29.022 kb on - strand, within GFF2233 at 29.028 kb on + strand, within GFF2233 at 29.028 kb on + strand, within GFF2233 at 29.029 kb on - strand, within GFF2233 at 29.029 kb on - strand, within GFF2233 at 29.046 kb on - strand, within GFF2233 at 29.048 kb on + strand, within GFF2233 at 29.049 kb on - strand, within GFF2233 at 29.094 kb on - strand, within GFF2233 at 29.119 kb on - strand, within GFF2233 at 29.214 kb on - strand, within GFF2233 at 29.238 kb on - strand, within GFF2233 at 29.238 kb on - strand, within GFF2233 at 29.238 kb on - strand, within GFF2233 at 29.241 kb on + strand, within GFF2233 at 29.242 kb on - strand, within GFF2233 at 29.269 kb on + strand, within GFF2233 at 29.277 kb on + strand, within GFF2233 at 29.443 kb on - strand at 29.460 kb on + strand at 29.461 kb on - strand at 29.743 kb on + strand at 29.812 kb on - strand, within GFF2234 at 29.819 kb on + strand, within GFF2234 at 29.872 kb on + strand, within GFF2234 at 29.900 kb on - strand, within GFF2234 at 29.911 kb on + strand, within GFF2234 at 30.002 kb on + strand, within GFF2234 at 30.010 kb on + strand, within GFF2234 at 30.169 kb on - strand, within GFF2234 at 30.216 kb on + strand, within GFF2234 at 30.221 kb on - strand, within GFF2234 at 30.309 kb on + strand, within GFF2234 at 30.310 kb on - strand, within GFF2234 at 30.315 kb on + strand, within GFF2234 at 30.315 kb on + strand, within GFF2234 at 30.531 kb on + strand at 30.531 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 26,603 - GFF2232 0.32 -0.1 26,735 + GFF2232 0.42 -0.9 26,744 + GFF2232 0.42 -0.2 26,747 + GFF2232 0.42 +0.4 26,788 - GFF2232 0.45 -0.4 26,811 + GFF2232 0.47 -1.4 26,855 - GFF2232 0.50 -0.1 26,855 - GFF2232 0.50 +1.8 26,931 + GFF2232 0.56 -0.2 26,936 - GFF2232 0.56 -0.1 26,975 + GFF2232 0.59 +0.0 27,030 + GFF2232 0.63 -1.8 27,169 - GFF2232 0.73 -1.4 27,169 - GFF2232 0.73 +0.3 27,169 - GFF2232 0.73 -0.5 27,228 + GFF2232 0.77 +0.3 27,342 + GFF2232 0.85 +0.4 27,459 + +0.5 27,466 + +1.3 27,591 - +0.6 27,714 + +0.0 27,714 + -0.9 27,715 - -2.0 27,807 - GFF2233 0.11 +0.8 27,813 - GFF2233 0.11 +0.7 27,824 + GFF2233 0.12 -0.1 27,930 + GFF2233 0.17 +0.6 27,964 - GFF2233 0.18 -1.0 28,313 + GFF2233 0.36 -2.3 28,314 - GFF2233 0.36 +0.1 28,314 - GFF2233 0.36 -2.2 28,538 + GFF2233 0.47 -0.2 28,617 - GFF2233 0.50 -0.6 28,626 + GFF2233 0.51 -0.3 28,628 + GFF2233 0.51 +0.5 28,628 + GFF2233 0.51 +0.8 28,629 - GFF2233 0.51 +0.6 28,689 - GFF2233 0.54 +0.5 28,773 + GFF2233 0.58 +0.5 28,897 + GFF2233 0.64 -2.6 28,898 - GFF2233 0.64 -0.1 28,932 - GFF2233 0.66 -0.3 28,932 - GFF2233 0.66 +0.5 28,955 - GFF2233 0.67 -2.1 28,955 - GFF2233 0.67 -1.6 28,965 + GFF2233 0.68 +0.7 29,021 + GFF2233 0.70 -2.0 29,022 - GFF2233 0.70 -0.2 29,028 + GFF2233 0.71 +1.4 29,028 + GFF2233 0.71 -0.1 29,029 - GFF2233 0.71 +1.8 29,029 - GFF2233 0.71 +1.4 29,046 - GFF2233 0.72 +1.3 29,048 + GFF2233 0.72 +0.9 29,049 - GFF2233 0.72 +0.3 29,094 - GFF2233 0.74 +1.5 29,119 - GFF2233 0.75 +0.9 29,214 - GFF2233 0.80 -1.0 29,238 - GFF2233 0.81 -1.6 29,238 - GFF2233 0.81 -2.0 29,238 - GFF2233 0.81 +3.1 29,241 + GFF2233 0.81 +1.6 29,242 - GFF2233 0.81 +0.4 29,269 + GFF2233 0.82 +2.4 29,277 + GFF2233 0.83 -3.4 29,443 - -0.4 29,460 + +0.8 29,461 - +0.3 29,743 + +0.7 29,812 - GFF2234 0.17 -0.3 29,819 + GFF2234 0.18 +0.4 29,872 + GFF2234 0.24 +0.5 29,900 - GFF2234 0.27 -0.6 29,911 + GFF2234 0.29 -0.2 30,002 + GFF2234 0.40 -0.3 30,010 + GFF2234 0.40 -0.9 30,169 - GFF2234 0.59 -0.6 30,216 + GFF2234 0.65 -0.1 30,221 - GFF2234 0.65 -0.4 30,309 + GFF2234 0.76 -0.6 30,310 - GFF2234 0.76 -0.5 30,315 + GFF2234 0.77 +1.1 30,315 + GFF2234 0.77 -0.4 30,531 + -0.5 30,531 + +0.6
Or see this region's nucleotide sequence