Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2166 and GFF2167 are separated by 4 nucleotides GFF2167 and GFF2168 are separated by 188 nucleotides GFF2168 and GFF2169 overlap by 1 nucleotides
GFF2166 - Exopolyphosphatase (EC 3.6.1.11), at 12,835 to 14,376
GFF2166
GFF2167 - Polyphosphate kinase (EC 2.7.4.1), at 14,381 to 16,447
GFF2167
GFF2168 - Phosphoribosylglycinamide formyltransferase (EC 2.1.2.2), at 16,636 to 17,274
GFF2168
GFF2169 - Phosphoribosylformylglycinamidine cyclo-ligase (EC 6.3.3.1), at 17,274 to 18,311
GFF2169
Position (kb) 14
15
16
17 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 13.401 kb on - strand, within GFF2166 at 13.401 kb on - strand, within GFF2166 at 13.475 kb on - strand, within GFF2166 at 13.717 kb on - strand, within GFF2166 at 13.759 kb on - strand, within GFF2166 at 13.850 kb on + strand, within GFF2166 at 13.984 kb on - strand, within GFF2166 at 14.101 kb on + strand, within GFF2166 at 14.106 kb on + strand, within GFF2166 at 14.116 kb on + strand, within GFF2166 at 14.122 kb on - strand, within GFF2166 at 14.150 kb on - strand, within GFF2166 at 14.178 kb on + strand, within GFF2166 at 14.178 kb on + strand, within GFF2166 at 14.215 kb on - strand, within GFF2166 at 14.254 kb on + strand at 14.304 kb on + strand at 14.304 kb on + strand at 14.410 kb on - strand at 14.410 kb on - strand at 14.440 kb on + strand at 14.638 kb on + strand, within GFF2167 at 14.659 kb on + strand, within GFF2167 at 14.659 kb on + strand, within GFF2167 at 14.904 kb on + strand, within GFF2167 at 15.414 kb on + strand, within GFF2167 at 15.414 kb on + strand, within GFF2167 at 15.415 kb on - strand, within GFF2167 at 15.417 kb on + strand, within GFF2167 at 15.418 kb on - strand, within GFF2167 at 15.657 kb on + strand, within GFF2167 at 15.988 kb on - strand, within GFF2167 at 16.045 kb on + strand, within GFF2167 at 16.045 kb on + strand, within GFF2167 at 16.067 kb on - strand, within GFF2167 at 16.428 kb on + strand at 16.429 kb on - strand at 16.452 kb on - strand at 16.456 kb on - strand at 16.467 kb on + strand at 16.489 kb on - strand at 16.505 kb on - strand at 16.511 kb on + strand at 16.634 kb on + strand at 16.708 kb on + strand, within GFF2168 at 16.731 kb on + strand, within GFF2168 at 16.732 kb on - strand, within GFF2168 at 16.782 kb on + strand, within GFF2168 at 16.783 kb on - strand, within GFF2168 at 17.004 kb on + strand, within GFF2168 at 17.004 kb on + strand, within GFF2168 at 17.042 kb on + strand, within GFF2168 at 17.234 kb on - strand at 17.234 kb on - strand at 17.269 kb on - strand at 17.424 kb on - strand, within GFF2169 at 17.429 kb on - strand, within GFF2169 at 17.429 kb on - strand, within GFF2169
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 13,401 - GFF2166 0.37 -1.9 13,401 - GFF2166 0.37 -0.7 13,475 - GFF2166 0.42 +0.3 13,717 - GFF2166 0.57 -0.2 13,759 - GFF2166 0.60 -0.0 13,850 + GFF2166 0.66 +0.7 13,984 - GFF2166 0.75 -2.2 14,101 + GFF2166 0.82 -2.6 14,106 + GFF2166 0.82 +1.5 14,116 + GFF2166 0.83 -2.5 14,122 - GFF2166 0.83 -0.1 14,150 - GFF2166 0.85 +0.4 14,178 + GFF2166 0.87 -0.2 14,178 + GFF2166 0.87 +0.7 14,215 - GFF2166 0.89 +0.1 14,254 + +0.4 14,304 + +0.6 14,304 + -0.1 14,410 - +0.2 14,410 - +0.5 14,440 + -1.7 14,638 + GFF2167 0.12 +0.6 14,659 + GFF2167 0.13 -0.6 14,659 + GFF2167 0.13 -2.2 14,904 + GFF2167 0.25 -0.2 15,414 + GFF2167 0.50 -1.0 15,414 + GFF2167 0.50 -0.1 15,415 - GFF2167 0.50 -0.3 15,417 + GFF2167 0.50 +0.1 15,418 - GFF2167 0.50 -1.3 15,657 + GFF2167 0.62 -0.6 15,988 - GFF2167 0.78 +0.3 16,045 + GFF2167 0.81 -0.9 16,045 + GFF2167 0.81 -1.6 16,067 - GFF2167 0.82 -1.4 16,428 + -0.0 16,429 - +0.1 16,452 - +0.1 16,456 - +0.7 16,467 + -1.2 16,489 - +0.1 16,505 - -0.1 16,511 + +2.3 16,634 + +0.6 16,708 + GFF2168 0.11 +0.6 16,731 + GFF2168 0.15 -0.9 16,732 - GFF2168 0.15 -3.1 16,782 + GFF2168 0.23 +0.0 16,783 - GFF2168 0.23 -0.9 17,004 + GFF2168 0.58 +0.1 17,004 + GFF2168 0.58 +3.7 17,042 + GFF2168 0.64 +0.1 17,234 - +0.4 17,234 - -0.1 17,269 - +0.4 17,424 - GFF2169 0.14 +0.4 17,429 - GFF2169 0.15 +0.4 17,429 - GFF2169 0.15 +1.1
Or see this region's nucleotide sequence