Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2109 and GFF2110 overlap by 4 nucleotides GFF2110 and GFF2111 are separated by 142 nucleotides GFF2111 and GFF2112 are separated by 217 nucleotides
GFF2109 - Sigma factor RpoE negative regulatory protein RseB precursor, at 17,938 to 18,894
GFF2109
GFF2110 - Sigma factor RpoE regulatory protein RseC, at 18,891 to 19,370
GFF2110
GFF2111 - FIG00643583: hypothetical protein, at 19,513 to 19,650
GFF2111
GFF2112 - Phage integrase, at 19,868 to 21,097
GFF2112
Position (kb) 19
20
21
22 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 18.888 kb on - strand at 19.037 kb on + strand, within GFF2110 at 19.038 kb on - strand, within GFF2110 at 19.137 kb on - strand, within GFF2110 at 19.137 kb on - strand, within GFF2110 at 19.491 kb on - strand at 19.491 kb on - strand at 19.496 kb on - strand at 19.515 kb on - strand at 19.523 kb on - strand at 19.523 kb on - strand at 19.600 kb on + strand, within GFF2111 at 19.601 kb on - strand, within GFF2111 at 19.883 kb on - strand at 19.919 kb on - strand at 20.077 kb on - strand, within GFF2112 at 20.080 kb on + strand, within GFF2112 at 20.199 kb on + strand, within GFF2112 at 20.269 kb on + strand, within GFF2112 at 20.269 kb on + strand, within GFF2112 at 20.273 kb on + strand, within GFF2112 at 20.363 kb on - strand, within GFF2112 at 20.363 kb on - strand, within GFF2112 at 20.382 kb on - strand, within GFF2112 at 20.557 kb on + strand, within GFF2112 at 20.681 kb on - strand, within GFF2112 at 20.728 kb on - strand, within GFF2112 at 20.850 kb on - strand, within GFF2112 at 20.886 kb on + strand, within GFF2112 at 20.886 kb on + strand, within GFF2112 at 20.886 kb on + strand, within GFF2112 at 20.932 kb on + strand, within GFF2112 at 20.932 kb on + strand, within GFF2112 at 20.933 kb on - strand, within GFF2112 at 20.933 kb on - strand, within GFF2112 at 20.981 kb on + strand at 20.990 kb on + strand at 20.991 kb on - strand at 20.996 kb on + strand at 20.999 kb on - strand at 21.020 kb on + strand at 21.022 kb on + strand at 21.022 kb on + strand at 21.219 kb on - strand at 21.220 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 18,888 - -0.5 19,037 + GFF2110 0.30 -0.6 19,038 - GFF2110 0.31 -0.1 19,137 - GFF2110 0.51 -1.2 19,137 - GFF2110 0.51 +0.6 19,491 - +0.4 19,491 - -0.3 19,496 - +0.2 19,515 - +0.2 19,523 - +0.6 19,523 - +0.1 19,600 + GFF2111 0.63 -0.8 19,601 - GFF2111 0.64 +0.3 19,883 - -0.3 19,919 - +0.2 20,077 - GFF2112 0.17 -2.3 20,080 + GFF2112 0.17 +0.5 20,199 + GFF2112 0.27 -0.0 20,269 + GFF2112 0.33 -0.0 20,269 + GFF2112 0.33 -0.0 20,273 + GFF2112 0.33 +1.0 20,363 - GFF2112 0.40 +0.6 20,363 - GFF2112 0.40 +0.7 20,382 - GFF2112 0.42 -0.2 20,557 + GFF2112 0.56 -0.5 20,681 - GFF2112 0.66 +3.1 20,728 - GFF2112 0.70 -0.4 20,850 - GFF2112 0.80 -1.6 20,886 + GFF2112 0.83 -1.1 20,886 + GFF2112 0.83 -0.8 20,886 + GFF2112 0.83 +0.3 20,932 + GFF2112 0.87 +1.4 20,932 + GFF2112 0.87 -0.8 20,933 - GFF2112 0.87 +0.8 20,933 - GFF2112 0.87 +0.7 20,981 + -0.4 20,990 + +0.3 20,991 - -2.2 20,996 + -0.2 20,999 - -0.3 21,020 + -2.6 21,022 + -0.7 21,022 + -0.9 21,219 - +0.8 21,220 + -1.1
Or see this region's nucleotide sequence