Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2035 and GFF2036 are separated by 54 nucleotides GFF2036 and GFF2037 are separated by 255 nucleotides
GFF2035 - Xanthosine permease, at 1,566 to 2,822
GFF2035
GFF2036 - Xanthosine phosphorylase (EC 2.4.2.1), at 2,877 to 3,710
GFF2036
GFF2037 - Putative exported protein precursor, at 3,966 to 4,727
GFF2037
Position (kb)
2
3
4 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1.961 kb on + strand, within GFF2035 at 1.997 kb on + strand, within GFF2035 at 2.144 kb on + strand, within GFF2035 at 2.338 kb on - strand, within GFF2035 at 2.470 kb on + strand, within GFF2035 at 2.470 kb on + strand, within GFF2035 at 2.506 kb on + strand, within GFF2035 at 2.506 kb on + strand, within GFF2035 at 2.506 kb on + strand, within GFF2035 at 2.506 kb on + strand, within GFF2035 at 2.507 kb on - strand, within GFF2035 at 2.507 kb on - strand, within GFF2035 at 2.569 kb on + strand, within GFF2035 at 2.608 kb on + strand, within GFF2035 at 2.609 kb on - strand, within GFF2035 at 2.609 kb on - strand, within GFF2035 at 2.654 kb on + strand, within GFF2035 at 2.741 kb on - strand at 2.741 kb on - strand at 2.741 kb on - strand at 2.844 kb on + strand at 2.889 kb on - strand at 2.889 kb on - strand at 2.892 kb on + strand at 2.913 kb on + strand at 2.971 kb on - strand, within GFF2036 at 3.220 kb on + strand, within GFF2036 at 3.221 kb on - strand, within GFF2036 at 3.296 kb on + strand, within GFF2036 at 3.297 kb on - strand, within GFF2036 at 3.468 kb on - strand, within GFF2036 at 3.666 kb on - strand at 4.066 kb on - strand, within GFF2037 at 4.289 kb on - strand, within GFF2037 at 4.318 kb on - strand, within GFF2037 at 4.338 kb on - strand, within GFF2037 at 4.369 kb on + strand, within GFF2037 at 4.377 kb on - strand, within GFF2037 at 4.427 kb on + strand, within GFF2037 at 4.443 kb on + strand, within GFF2037 at 4.462 kb on - strand, within GFF2037 at 4.462 kb on - strand, within GFF2037 at 4.466 kb on - strand, within GFF2037 at 4.468 kb on + strand, within GFF2037 at 4.488 kb on - strand, within GFF2037 at 4.536 kb on + strand, within GFF2037 at 4.560 kb on + strand, within GFF2037 at 4.700 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 1,961 + GFF2035 0.31 -3.4 1,997 + GFF2035 0.34 +1.1 2,144 + GFF2035 0.46 -0.3 2,338 - GFF2035 0.61 -0.4 2,470 + GFF2035 0.72 +0.5 2,470 + GFF2035 0.72 +0.8 2,506 + GFF2035 0.75 +0.5 2,506 + GFF2035 0.75 -0.7 2,506 + GFF2035 0.75 -0.7 2,506 + GFF2035 0.75 +0.7 2,507 - GFF2035 0.75 +1.0 2,507 - GFF2035 0.75 +0.1 2,569 + GFF2035 0.80 +0.2 2,608 + GFF2035 0.83 +0.8 2,609 - GFF2035 0.83 +1.9 2,609 - GFF2035 0.83 -0.4 2,654 + GFF2035 0.87 -0.8 2,741 - +0.6 2,741 - +0.1 2,741 - +0.7 2,844 + +0.1 2,889 - -0.4 2,889 - -0.4 2,892 + -0.6 2,913 + +1.0 2,971 - GFF2036 0.11 +0.8 3,220 + GFF2036 0.41 +0.4 3,221 - GFF2036 0.41 -0.2 3,296 + GFF2036 0.50 +3.0 3,297 - GFF2036 0.50 +1.2 3,468 - GFF2036 0.71 +0.9 3,666 - +1.6 4,066 - GFF2037 0.13 -1.8 4,289 - GFF2037 0.42 -1.4 4,318 - GFF2037 0.46 +1.5 4,338 - GFF2037 0.49 -0.3 4,369 + GFF2037 0.53 +0.0 4,377 - GFF2037 0.54 -0.5 4,427 + GFF2037 0.60 +0.5 4,443 + GFF2037 0.63 -0.3 4,462 - GFF2037 0.65 -0.7 4,462 - GFF2037 0.65 -0.3 4,466 - GFF2037 0.66 -0.3 4,468 + GFF2037 0.66 -1.3 4,488 - GFF2037 0.69 +1.5 4,536 + GFF2037 0.75 +0.1 4,560 + GFF2037 0.78 -0.5 4,700 + -0.2
Or see this region's nucleotide sequence