Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1974 and GFF1975 are separated by 179 nucleotides GFF1975 and GFF1976 are separated by 113 nucleotides GFF1976 and GFF1977 are separated by 60 nucleotides
GFF1974 - Pyruvate decarboxylase (EC 4.1.1.1); Alpha-keto-acid decarboxylase (EC 4.1.1.-), at 166,778 to 168,430
GFF1974
GFF1975 - Putative ion-channel protein, at 168,610 to 169,608
GFF1975
GFF1976 - FIG00639029: hypothetical protein, at 169,722 to 170,048
GFF1976
GFF1977 - Manganese transport protein MntH, at 170,109 to 171,287
GFF1977
Position (kb) 168
169
170 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 167.610 kb on - strand, within GFF1974 at 167.649 kb on + strand, within GFF1974 at 167.649 kb on + strand, within GFF1974 at 167.702 kb on + strand, within GFF1974 at 167.940 kb on + strand, within GFF1974 at 167.941 kb on - strand, within GFF1974 at 168.011 kb on + strand, within GFF1974 at 168.012 kb on - strand, within GFF1974 at 168.184 kb on + strand, within GFF1974 at 168.219 kb on - strand, within GFF1974 at 168.416 kb on - strand at 168.454 kb on + strand at 168.455 kb on - strand at 168.457 kb on + strand at 168.457 kb on + strand at 168.526 kb on + strand at 168.542 kb on - strand at 168.576 kb on + strand at 168.577 kb on - strand at 168.806 kb on - strand, within GFF1975 at 169.015 kb on + strand, within GFF1975 at 169.068 kb on - strand, within GFF1975 at 169.099 kb on + strand, within GFF1975 at 169.114 kb on - strand, within GFF1975 at 169.117 kb on + strand, within GFF1975 at 169.143 kb on + strand, within GFF1975 at 169.192 kb on - strand, within GFF1975 at 169.195 kb on + strand, within GFF1975 at 169.306 kb on + strand, within GFF1975 at 169.307 kb on - strand, within GFF1975 at 169.374 kb on + strand, within GFF1975 at 169.721 kb on + strand at 169.848 kb on + strand, within GFF1976 at 169.848 kb on + strand, within GFF1976 at 169.955 kb on + strand, within GFF1976 at 170.047 kb on - strand at 170.148 kb on - strand at 170.176 kb on - strand at 170.229 kb on - strand, within GFF1977 at 170.360 kb on + strand, within GFF1977 at 170.437 kb on - strand, within GFF1977 at 170.472 kb on - strand, within GFF1977 at 170.503 kb on - strand, within GFF1977 at 170.524 kb on + strand, within GFF1977
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 167,610 - GFF1974 0.50 +0.2 167,649 + GFF1974 0.53 +0.2 167,649 + GFF1974 0.53 +0.4 167,702 + GFF1974 0.56 +0.2 167,940 + GFF1974 0.70 -0.8 167,941 - GFF1974 0.70 +1.4 168,011 + GFF1974 0.75 -1.1 168,012 - GFF1974 0.75 -1.1 168,184 + GFF1974 0.85 -3.1 168,219 - GFF1974 0.87 +1.4 168,416 - +0.6 168,454 + -0.4 168,455 - -1.4 168,457 + +0.1 168,457 + -0.6 168,526 + +0.2 168,542 - +0.4 168,576 + -1.8 168,577 - -0.1 168,806 - GFF1975 0.20 +1.0 169,015 + GFF1975 0.41 -0.0 169,068 - GFF1975 0.46 +1.1 169,099 + GFF1975 0.49 +0.9 169,114 - GFF1975 0.50 +0.6 169,117 + GFF1975 0.51 -0.8 169,143 + GFF1975 0.53 -0.3 169,192 - GFF1975 0.58 -0.7 169,195 + GFF1975 0.59 +1.6 169,306 + GFF1975 0.70 +0.3 169,307 - GFF1975 0.70 +0.5 169,374 + GFF1975 0.76 -0.7 169,721 + +0.6 169,848 + GFF1976 0.39 +0.4 169,848 + GFF1976 0.39 -0.2 169,955 + GFF1976 0.71 +0.6 170,047 - -1.2 170,148 - +0.0 170,176 - +2.1 170,229 - GFF1977 0.10 -2.7 170,360 + GFF1977 0.21 +1.2 170,437 - GFF1977 0.28 +1.2 170,472 - GFF1977 0.31 -0.2 170,503 - GFF1977 0.33 -1.1 170,524 + GFF1977 0.35 -0.8
Or see this region's nucleotide sequence