Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1863 and GFF1864 overlap by 1 nucleotides GFF1864 and GFF1865 overlap by 6 nucleotides GFF1865 and GFF1866 are separated by 154 nucleotides GFF1866 and GFF1867 overlap by 4 nucleotides GFF1867 and GFF1868 overlap by 1 nucleotides
GFF1863 - Polymyxin resistance protein PmrL, sucrose-6 phosphate hydrolase, at 57,004 to 57,339
GFF1863
GFF1864 - Polymyxin resistance protein PmrM, at 57,339 to 57,716
GFF1864
GFF1865 - Polymyxin resistance protein PmrD, at 57,711 to 57,911
GFF1865
GFF1866 - O-succinylbenzoic acid--CoA ligase (EC 6.2.1.26), at 58,066 to 59,433
GFF1866
GFF1867 - O-succinylbenzoate synthase (EC 4.2.1.113), at 59,430 to 60,392
GFF1867
GFF1868 - Naphthoate synthase (EC 4.1.3.36), at 60,392 to 61,249
GFF1868
Position (kb)
58
59
60 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 57.137 kb on + strand, within GFF1863 at 57.487 kb on + strand, within GFF1864 at 57.755 kb on - strand, within GFF1865 at 57.856 kb on - strand, within GFF1865 at 57.856 kb on - strand, within GFF1865 at 58.119 kb on + strand at 58.235 kb on + strand, within GFF1866 at 58.236 kb on - strand, within GFF1866 at 58.606 kb on - strand, within GFF1866 at 58.779 kb on + strand, within GFF1866 at 58.780 kb on - strand, within GFF1866 at 58.857 kb on + strand, within GFF1866 at 58.858 kb on - strand, within GFF1866 at 58.858 kb on - strand, within GFF1866 at 58.858 kb on - strand, within GFF1866 at 58.860 kb on + strand, within GFF1866 at 58.861 kb on - strand, within GFF1866 at 58.861 kb on - strand, within GFF1866 at 58.971 kb on + strand, within GFF1866 at 58.971 kb on + strand, within GFF1866 at 58.971 kb on + strand, within GFF1866 at 58.972 kb on - strand, within GFF1866 at 58.972 kb on - strand, within GFF1866 at 58.972 kb on - strand, within GFF1866 at 58.975 kb on + strand, within GFF1866 at 58.976 kb on - strand, within GFF1866 at 58.976 kb on - strand, within GFF1866 at 58.976 kb on - strand, within GFF1866 at 58.976 kb on - strand, within GFF1866 at 59.075 kb on - strand, within GFF1866 at 59.288 kb on - strand, within GFF1866 at 59.523 kb on - strand at 59.664 kb on - strand, within GFF1867 at 59.685 kb on - strand, within GFF1867 at 59.864 kb on - strand, within GFF1867 at 59.864 kb on - strand, within GFF1867 at 60.202 kb on - strand, within GFF1867 at 60.202 kb on - strand, within GFF1867 at 60.202 kb on - strand, within GFF1867 at 60.302 kb on - strand at 60.376 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 57,137 + GFF1863 0.40 -0.5 57,487 + GFF1864 0.39 +0.3 57,755 - GFF1865 0.22 +4.0 57,856 - GFF1865 0.72 -0.0 57,856 - GFF1865 0.72 -1.2 58,119 + -3.7 58,235 + GFF1866 0.12 +0.6 58,236 - GFF1866 0.12 -1.2 58,606 - GFF1866 0.39 +0.7 58,779 + GFF1866 0.52 +1.1 58,780 - GFF1866 0.52 -1.1 58,857 + GFF1866 0.58 +0.4 58,858 - GFF1866 0.58 +0.5 58,858 - GFF1866 0.58 +0.8 58,858 - GFF1866 0.58 -0.4 58,860 + GFF1866 0.58 +0.3 58,861 - GFF1866 0.58 +1.0 58,861 - GFF1866 0.58 +0.4 58,971 + GFF1866 0.66 -0.4 58,971 + GFF1866 0.66 +1.6 58,971 + GFF1866 0.66 +0.2 58,972 - GFF1866 0.66 +2.5 58,972 - GFF1866 0.66 +3.1 58,972 - GFF1866 0.66 +1.4 58,975 + GFF1866 0.66 -0.4 58,976 - GFF1866 0.67 +0.6 58,976 - GFF1866 0.67 -0.4 58,976 - GFF1866 0.67 +0.1 58,976 - GFF1866 0.67 +0.8 59,075 - GFF1866 0.74 +0.6 59,288 - GFF1866 0.89 +0.7 59,523 - +1.1 59,664 - GFF1867 0.24 +0.6 59,685 - GFF1867 0.26 +0.3 59,864 - GFF1867 0.45 +1.2 59,864 - GFF1867 0.45 +0.2 60,202 - GFF1867 0.80 -0.2 60,202 - GFF1867 0.80 +3.6 60,202 - GFF1867 0.80 -0.4 60,302 - -0.8 60,376 - +0.6
Or see this region's nucleotide sequence