Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1762 and GFF1763 overlap by 4 nucleotides GFF1763 and GFF1764 overlap by 17 nucleotides GFF1764 and GFF1765 overlap by 4 nucleotides
GFF1762 - D-Galactonate repressor DgoR, at 65,895 to 66,584
GFF1762
GFF1763 - 2-dehydro-3-deoxygalactonokinase (EC 2.7.1.58), at 66,581 to 67,459
GFF1763
GFF1764 - 2-dehydro-3-deoxyphosphogalactonate aldolase (EC 4.1.2.21), at 67,443 to 68,060
GFF1764
GFF1765 - Galactonate dehydratase (EC 4.2.1.6), at 68,057 to 69,205
GFF1765
Position (kb) 67
68
69 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 66.479 kb on + strand, within GFF1762 at 66.479 kb on + strand, within GFF1762 at 66.479 kb on + strand, within GFF1762 at 66.480 kb on - strand, within GFF1762 at 66.593 kb on + strand at 66.594 kb on - strand at 66.701 kb on - strand, within GFF1763 at 66.701 kb on - strand, within GFF1763 at 66.787 kb on + strand, within GFF1763 at 66.792 kb on + strand, within GFF1763 at 66.856 kb on - strand, within GFF1763 at 66.983 kb on + strand, within GFF1763 at 66.984 kb on - strand, within GFF1763 at 67.166 kb on - strand, within GFF1763 at 67.356 kb on - strand, within GFF1763 at 67.369 kb on + strand, within GFF1763 at 67.369 kb on + strand, within GFF1763 at 67.458 kb on - strand at 67.475 kb on + strand at 67.480 kb on + strand at 67.487 kb on + strand at 67.488 kb on - strand at 67.547 kb on + strand, within GFF1764 at 67.549 kb on + strand, within GFF1764 at 67.550 kb on - strand, within GFF1764 at 67.609 kb on - strand, within GFF1764 at 67.642 kb on + strand, within GFF1764 at 67.660 kb on + strand, within GFF1764 at 67.661 kb on - strand, within GFF1764 at 67.740 kb on + strand, within GFF1764 at 67.741 kb on - strand, within GFF1764 at 67.741 kb on - strand, within GFF1764 at 68.054 kb on + strand at 68.081 kb on + strand at 68.082 kb on - strand at 68.088 kb on + strand at 68.174 kb on - strand, within GFF1765 at 68.190 kb on + strand, within GFF1765 at 68.191 kb on - strand, within GFF1765 at 68.191 kb on - strand, within GFF1765 at 68.208 kb on - strand, within GFF1765 at 68.253 kb on + strand, within GFF1765 at 68.254 kb on - strand, within GFF1765 at 68.254 kb on - strand, within GFF1765 at 68.406 kb on - strand, within GFF1765 at 68.406 kb on - strand, within GFF1765 at 68.408 kb on - strand, within GFF1765 at 68.678 kb on - strand, within GFF1765 at 68.765 kb on - strand, within GFF1765 at 68.765 kb on - strand, within GFF1765 at 68.765 kb on - strand, within GFF1765 at 68.861 kb on + strand, within GFF1765 at 68.888 kb on + strand, within GFF1765 at 68.888 kb on + strand, within GFF1765 at 68.889 kb on - strand, within GFF1765 at 69.005 kb on + strand, within GFF1765 at 69.007 kb on + strand, within GFF1765 at 69.007 kb on + strand, within GFF1765 at 69.008 kb on - strand, within GFF1765
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 66,479 + GFF1762 0.85 -0.0 66,479 + GFF1762 0.85 -3.0 66,479 + GFF1762 0.85 +0.5 66,480 - GFF1762 0.85 -1.2 66,593 + +0.1 66,594 - +0.5 66,701 - GFF1763 0.14 +1.0 66,701 - GFF1763 0.14 +1.1 66,787 + GFF1763 0.23 -0.6 66,792 + GFF1763 0.24 -0.6 66,856 - GFF1763 0.31 +1.4 66,983 + GFF1763 0.46 -1.2 66,984 - GFF1763 0.46 -1.6 67,166 - GFF1763 0.67 +0.9 67,356 - GFF1763 0.88 +0.6 67,369 + GFF1763 0.90 +2.6 67,369 + GFF1763 0.90 -0.3 67,458 - -0.0 67,475 + +1.4 67,480 + +0.6 67,487 + +0.2 67,488 - +0.1 67,547 + GFF1764 0.17 -0.7 67,549 + GFF1764 0.17 -1.5 67,550 - GFF1764 0.17 +0.4 67,609 - GFF1764 0.27 -0.7 67,642 + GFF1764 0.32 -0.9 67,660 + GFF1764 0.35 -1.1 67,661 - GFF1764 0.35 +0.7 67,740 + GFF1764 0.48 +0.8 67,741 - GFF1764 0.48 +0.2 67,741 - GFF1764 0.48 -0.3 68,054 + +1.6 68,081 + +1.0 68,082 - -1.4 68,088 + +0.6 68,174 - GFF1765 0.10 +0.7 68,190 + GFF1765 0.12 +0.5 68,191 - GFF1765 0.12 -1.0 68,191 - GFF1765 0.12 +0.5 68,208 - GFF1765 0.13 +1.6 68,253 + GFF1765 0.17 -1.6 68,254 - GFF1765 0.17 +2.3 68,254 - GFF1765 0.17 -0.6 68,406 - GFF1765 0.30 +1.1 68,406 - GFF1765 0.30 +0.4 68,408 - GFF1765 0.31 +0.5 68,678 - GFF1765 0.54 -1.5 68,765 - GFF1765 0.62 -0.7 68,765 - GFF1765 0.62 -0.1 68,765 - GFF1765 0.62 +1.4 68,861 + GFF1765 0.70 -1.7 68,888 + GFF1765 0.72 +1.2 68,888 + GFF1765 0.72 +0.8 68,889 - GFF1765 0.72 -0.0 69,005 + GFF1765 0.83 +1.1 69,007 + GFF1765 0.83 -1.1 69,007 + GFF1765 0.83 -0.1 69,008 - GFF1765 0.83 +1.2
Or see this region's nucleotide sequence