Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1750

Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1748 and GFF1749 are separated by 15 nucleotidesGFF1749 and GFF1750 are separated by 255 nucleotidesGFF1750 and GFF1751 are separated by 141 nucleotides GFF1748 - Integrase, at 48,786 to 49,496 GFF1748 GFF1749 - Integrase, at 49,512 to 50,006 GFF1749 GFF1750 - GTPase and tRNA-U34 5-formylation enzyme TrmE, at 50,262 to 51,626 GFF1750 GFF1751 - Inner membrane protein translocase component YidC, long form, at 51,768 to 53,414 GFF1751 Position (kb) 50 51 52Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 49.292 kb on + strand, within GFF1748at 49.350 kb on - strand, within GFF1748at 49.357 kb on + strand, within GFF1748at 49.358 kb on - strand, within GFF1748at 49.428 kb on - strandat 49.428 kb on - strandat 49.429 kb on + strandat 49.442 kb on + strandat 49.487 kb on - strandat 49.502 kb on + strandat 50.018 kb on - strandat 50.019 kb on + strandat 50.041 kb on - strandat 50.194 kb on + strandat 50.219 kb on - strandat 50.263 kb on + strandat 50.394 kb on + strandat 50.397 kb on + strandat 50.397 kb on + strandat 50.448 kb on + strand, within GFF1750at 50.449 kb on - strand, within GFF1750at 50.612 kb on + strand, within GFF1750at 50.624 kb on + strand, within GFF1750at 50.629 kb on - strand, within GFF1750at 50.826 kb on - strand, within GFF1750at 51.137 kb on + strand, within GFF1750at 51.137 kb on + strand, within GFF1750at 51.385 kb on - strand, within GFF1750

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
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49,292 + GFF1748 0.71 +0.6
49,350 - GFF1748 0.79 +0.9
49,357 + GFF1748 0.80 -0.1
49,358 - GFF1748 0.80 +0.3
49,428 - +0.4
49,428 - +0.6
49,429 + +0.5
49,442 + -0.7
49,487 - +0.4
49,502 + +0.6
50,018 - +2.0
50,019 + -0.3
50,041 - +0.5
50,194 + -2.1
50,219 - -1.2
50,263 + +1.1
50,394 + -0.2
50,397 + -1.4
50,397 + +0.1
50,448 + GFF1750 0.14 +0.1
50,449 - GFF1750 0.14 -2.1
50,612 + GFF1750 0.26 -0.4
50,624 + GFF1750 0.27 -2.1
50,629 - GFF1750 0.27 -1.1
50,826 - GFF1750 0.41 -1.5
51,137 + GFF1750 0.64 -1.4
51,137 + GFF1750 0.64 -2.9
51,385 - GFF1750 0.82 +1.4

Or see this region's nucleotide sequence