Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1530

Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1529 and GFF1530 are separated by 26 nucleotidesGFF1530 and GFF1531 are separated by 10 nucleotides GFF1529 - PTS system, galactose-inducible IIB component (EC 2.7.1.69) / PTS system, galactose-inducible IIC component (EC 2.7.1.69), at 32,720 to 34,147 GFF1529 GFF1530 - Tagatose-6-phosphate kinase (EC 2.7.1.144) / 1-phosphofructokinase (EC 2.7.1.56), at 34,174 to 35,088 GFF1530 GFF1531 - Tagatose 1,6-bisphosphate aldolase (EC 4.1.2.40), at 35,099 to 35,953 GFF1531 Position (kb) 34 35 36Strain fitness (log2 ratio) -1 0 1at 33.266 kb on + strand, within GFF1529at 33.314 kb on - strand, within GFF1529at 33.411 kb on + strand, within GFF1529at 33.522 kb on - strand, within GFF1529at 34.205 kb on - strandat 34.232 kb on - strandat 34.415 kb on + strand, within GFF1530at 35.040 kb on + strandat 35.047 kb on - strandat 35.064 kb on + strandat 35.406 kb on + strand, within GFF1531at 35.407 kb on - strand, within GFF1531at 35.519 kb on + strand, within GFF1531at 35.520 kb on - strand, within GFF1531at 35.791 kb on + strand, within GFF1531at 35.792 kb on - strand, within GFF1531at 35.792 kb on - strand, within GFF1531

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
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33,266 + GFF1529 0.38 +1.0
33,314 - GFF1529 0.42 +0.4
33,411 + GFF1529 0.48 +0.2
33,522 - GFF1529 0.56 +0.0
34,205 - +0.1
34,232 - +0.3
34,415 + GFF1530 0.26 +1.5
35,040 + -0.8
35,047 - -0.8
35,064 + +0.9
35,406 + GFF1531 0.36 -1.5
35,407 - GFF1531 0.36 +0.6
35,519 + GFF1531 0.49 +1.3
35,520 - GFF1531 0.49 +1.5
35,791 + GFF1531 0.81 +0.4
35,792 - GFF1531 0.81 -0.5
35,792 - GFF1531 0.81 -1.0

Or see this region's nucleotide sequence