Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1475 and GFF1476 are separated by 83 nucleotides GFF1476 and GFF1477 are separated by 97 nucleotides
GFF1475 - Alpha-galactosidase (EC 3.2.1.22), at 142,529 to 143,884
GFF1475
GFF1476 - Melibiose carrier protein, Na+/melibiose symporter, at 143,968 to 145,398
GFF1476
GFF1477 - Fumarate hydratase class I, anaerobic (EC 4.2.1.2), at 145,496 to 147,142
GFF1477
Position (kb)
143
144
145
146 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 143.009 kb on + strand, within GFF1475 at 143.009 kb on + strand, within GFF1475 at 143.010 kb on - strand, within GFF1475 at 143.010 kb on - strand, within GFF1475 at 143.056 kb on - strand, within GFF1475 at 143.093 kb on - strand, within GFF1475 at 143.183 kb on + strand, within GFF1475 at 143.184 kb on - strand, within GFF1475 at 143.357 kb on - strand, within GFF1475 at 143.427 kb on + strand, within GFF1475 at 143.450 kb on - strand, within GFF1475 at 143.468 kb on - strand, within GFF1475 at 143.522 kb on - strand, within GFF1475 at 143.522 kb on - strand, within GFF1475 at 143.572 kb on + strand, within GFF1475 at 143.762 kb on + strand at 143.875 kb on - strand at 143.926 kb on - strand at 143.926 kb on - strand at 143.928 kb on + strand at 143.967 kb on + strand at 143.999 kb on - strand at 144.043 kb on + strand at 144.043 kb on + strand at 144.043 kb on + strand at 144.044 kb on - strand at 144.044 kb on - strand at 144.045 kb on + strand at 144.045 kb on + strand at 144.045 kb on + strand at 144.046 kb on - strand at 144.046 kb on - strand at 144.046 kb on - strand at 144.049 kb on + strand at 144.049 kb on + strand at 144.049 kb on + strand at 144.049 kb on + strand at 144.050 kb on - strand at 144.050 kb on - strand at 144.050 kb on - strand at 144.059 kb on - strand at 144.059 kb on - strand at 144.062 kb on - strand at 144.287 kb on + strand, within GFF1476 at 144.287 kb on + strand, within GFF1476 at 144.288 kb on - strand, within GFF1476 at 144.304 kb on + strand, within GFF1476 at 144.304 kb on + strand, within GFF1476 at 144.325 kb on + strand, within GFF1476 at 144.326 kb on - strand, within GFF1476 at 144.327 kb on + strand, within GFF1476 at 144.327 kb on + strand, within GFF1476 at 144.328 kb on - strand, within GFF1476 at 144.367 kb on - strand, within GFF1476 at 144.369 kb on + strand, within GFF1476 at 144.452 kb on + strand, within GFF1476 at 144.472 kb on + strand, within GFF1476 at 144.513 kb on + strand, within GFF1476 at 144.521 kb on + strand, within GFF1476 at 144.569 kb on + strand, within GFF1476 at 144.569 kb on + strand, within GFF1476 at 144.570 kb on - strand, within GFF1476 at 144.570 kb on - strand, within GFF1476 at 144.570 kb on - strand, within GFF1476 at 144.600 kb on - strand, within GFF1476 at 144.712 kb on - strand, within GFF1476 at 144.737 kb on - strand, within GFF1476 at 144.746 kb on - strand, within GFF1476 at 144.746 kb on - strand, within GFF1476 at 144.778 kb on + strand, within GFF1476 at 145.051 kb on - strand, within GFF1476 at 145.072 kb on + strand, within GFF1476 at 145.204 kb on - strand, within GFF1476 at 145.207 kb on - strand, within GFF1476 at 145.262 kb on - strand at 145.315 kb on - strand at 145.319 kb on - strand at 145.606 kb on + strand at 145.651 kb on + strand at 145.652 kb on - strand at 145.702 kb on - strand, within GFF1477 at 145.772 kb on - strand, within GFF1477 at 145.803 kb on + strand, within GFF1477 at 145.804 kb on - strand, within GFF1477 at 145.804 kb on - strand, within GFF1477 at 145.804 kb on - strand, within GFF1477 at 145.836 kb on - strand, within GFF1477 at 145.900 kb on + strand, within GFF1477 at 145.900 kb on + strand, within GFF1477 at 145.903 kb on + strand, within GFF1477 at 145.904 kb on - strand, within GFF1477 at 145.905 kb on + strand, within GFF1477 at 146.107 kb on + strand, within GFF1477 at 146.109 kb on + strand, within GFF1477 at 146.110 kb on - strand, within GFF1477 at 146.110 kb on - strand, within GFF1477 at 146.243 kb on + strand, within GFF1477 at 146.386 kb on - strand, within GFF1477
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 143,009 + GFF1475 0.35 +0.5 143,009 + GFF1475 0.35 +0.1 143,010 - GFF1475 0.35 -0.1 143,010 - GFF1475 0.35 +0.2 143,056 - GFF1475 0.39 +0.5 143,093 - GFF1475 0.42 +0.9 143,183 + GFF1475 0.48 +0.3 143,184 - GFF1475 0.48 +0.1 143,357 - GFF1475 0.61 +0.1 143,427 + GFF1475 0.66 -3.5 143,450 - GFF1475 0.68 -0.2 143,468 - GFF1475 0.69 +1.0 143,522 - GFF1475 0.73 +1.4 143,522 - GFF1475 0.73 -0.3 143,572 + GFF1475 0.77 -0.7 143,762 + +0.3 143,875 - +1.3 143,926 - +0.1 143,926 - -1.3 143,928 + +0.1 143,967 + +2.0 143,999 - +1.0 144,043 + -3.0 144,043 + +0.1 144,043 + +1.5 144,044 - +1.5 144,044 - +2.2 144,045 + -1.2 144,045 + +1.5 144,045 + -0.8 144,046 - -0.1 144,046 - +0.0 144,046 - -0.8 144,049 + +1.2 144,049 + +2.8 144,049 + -1.6 144,049 + -0.2 144,050 - +0.6 144,050 - +0.9 144,050 - -0.5 144,059 - +0.3 144,059 - +2.0 144,062 - -0.6 144,287 + GFF1476 0.22 -0.3 144,287 + GFF1476 0.22 -0.4 144,288 - GFF1476 0.22 -1.6 144,304 + GFF1476 0.23 +0.4 144,304 + GFF1476 0.23 -0.6 144,325 + GFF1476 0.25 -2.0 144,326 - GFF1476 0.25 +1.3 144,327 + GFF1476 0.25 -0.7 144,327 + GFF1476 0.25 -0.1 144,328 - GFF1476 0.25 +1.3 144,367 - GFF1476 0.28 +0.4 144,369 + GFF1476 0.28 -0.6 144,452 + GFF1476 0.34 +0.4 144,472 + GFF1476 0.35 -0.9 144,513 + GFF1476 0.38 +0.1 144,521 + GFF1476 0.39 -2.7 144,569 + GFF1476 0.42 -2.8 144,569 + GFF1476 0.42 -0.1 144,570 - GFF1476 0.42 -0.7 144,570 - GFF1476 0.42 +1.1 144,570 - GFF1476 0.42 -3.0 144,600 - GFF1476 0.44 +0.7 144,712 - GFF1476 0.52 -0.3 144,737 - GFF1476 0.54 +0.9 144,746 - GFF1476 0.54 -1.0 144,746 - GFF1476 0.54 -0.2 144,778 + GFF1476 0.57 +0.2 145,051 - GFF1476 0.76 -1.0 145,072 + GFF1476 0.77 +1.0 145,204 - GFF1476 0.86 +1.8 145,207 - GFF1476 0.87 +1.1 145,262 - +0.0 145,315 - +0.4 145,319 - -0.4 145,606 + -0.2 145,651 + +0.6 145,652 - +0.9 145,702 - GFF1477 0.13 -0.6 145,772 - GFF1477 0.17 +1.2 145,803 + GFF1477 0.19 -0.4 145,804 - GFF1477 0.19 -0.9 145,804 - GFF1477 0.19 +0.9 145,804 - GFF1477 0.19 +0.8 145,836 - GFF1477 0.21 +0.3 145,900 + GFF1477 0.25 -3.2 145,900 + GFF1477 0.25 +0.2 145,903 + GFF1477 0.25 -1.5 145,904 - GFF1477 0.25 -2.8 145,905 + GFF1477 0.25 -0.1 146,107 + GFF1477 0.37 +0.5 146,109 + GFF1477 0.37 -1.6 146,110 - GFF1477 0.37 +0.1 146,110 - GFF1477 0.37 +1.3 146,243 + GFF1477 0.45 +0.7 146,386 - GFF1477 0.54 +1.2
Or see this region's nucleotide sequence