Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1443 and GFF1444 are separated by 147 nucleotides GFF1444 and GFF1445 are separated by 76 nucleotides
GFF1443 - Glutathione S-transferase (EC 2.5.1.18), at 105,262 to 105,930
GFF1443
GFF1444 - hypothetical protein, at 106,078 to 106,200
GFF1444
GFF1445 - Guanine-hypoxanthine permease, at 106,277 to 107,626
GFF1445
Position (kb)
106
107 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 105.141 kb on + strand at 105.146 kb on + strand at 105.229 kb on - strand at 105.234 kb on + strand at 105.243 kb on + strand at 105.243 kb on + strand at 105.244 kb on - strand at 105.282 kb on - strand at 105.282 kb on - strand at 105.488 kb on - strand, within GFF1443 at 105.538 kb on + strand, within GFF1443 at 105.551 kb on + strand, within GFF1443 at 105.765 kb on - strand, within GFF1443 at 105.769 kb on - strand, within GFF1443 at 105.769 kb on - strand, within GFF1443 at 105.840 kb on + strand, within GFF1443 at 105.841 kb on - strand, within GFF1443 at 105.864 kb on - strand at 105.960 kb on + strand at 105.960 kb on + strand at 105.960 kb on + strand at 106.049 kb on - strand at 106.141 kb on - strand, within GFF1444 at 106.142 kb on + strand, within GFF1444 at 106.206 kb on + strand at 106.207 kb on - strand at 106.229 kb on - strand at 106.240 kb on - strand at 106.240 kb on - strand at 106.240 kb on - strand at 106.276 kb on + strand at 106.430 kb on - strand, within GFF1445 at 106.493 kb on - strand, within GFF1445 at 106.499 kb on - strand, within GFF1445 at 106.541 kb on - strand, within GFF1445 at 106.558 kb on + strand, within GFF1445 at 106.588 kb on + strand, within GFF1445 at 106.588 kb on + strand, within GFF1445 at 106.633 kb on - strand, within GFF1445 at 106.831 kb on + strand, within GFF1445 at 106.856 kb on - strand, within GFF1445 at 106.906 kb on + strand, within GFF1445 at 106.918 kb on + strand, within GFF1445 at 107.035 kb on - strand, within GFF1445 at 107.126 kb on - strand, within GFF1445
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 105,141 + -1.2 105,146 + -0.0 105,229 - -0.7 105,234 + +0.1 105,243 + +1.4 105,243 + -0.7 105,244 - -0.5 105,282 - +0.5 105,282 - -0.3 105,488 - GFF1443 0.34 +1.0 105,538 + GFF1443 0.41 -1.7 105,551 + GFF1443 0.43 +0.5 105,765 - GFF1443 0.75 -1.5 105,769 - GFF1443 0.76 -0.2 105,769 - GFF1443 0.76 +0.4 105,840 + GFF1443 0.86 -0.1 105,841 - GFF1443 0.87 +1.2 105,864 - +2.1 105,960 + +0.6 105,960 + +1.1 105,960 + -0.6 106,049 - +1.4 106,141 - GFF1444 0.51 +0.6 106,142 + GFF1444 0.52 +0.9 106,206 + +0.4 106,207 - -1.2 106,229 - -1.5 106,240 - +0.3 106,240 - +0.6 106,240 - +1.8 106,276 + -0.6 106,430 - GFF1445 0.11 -1.9 106,493 - GFF1445 0.16 -2.8 106,499 - GFF1445 0.16 +0.4 106,541 - GFF1445 0.20 -0.2 106,558 + GFF1445 0.21 -0.2 106,588 + GFF1445 0.23 +0.4 106,588 + GFF1445 0.23 +0.3 106,633 - GFF1445 0.26 +1.0 106,831 + GFF1445 0.41 +0.9 106,856 - GFF1445 0.43 +1.4 106,906 + GFF1445 0.47 +0.7 106,918 + GFF1445 0.47 -0.5 107,035 - GFF1445 0.56 +0.7 107,126 - GFF1445 0.63 -0.2
Or see this region's nucleotide sequence