Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF136 and GFF137 are separated by 9 nucleotides GFF137 and GFF138 are separated by 153 nucleotides GFF138 and GFF139 are separated by 33 nucleotides GFF139 and GFF140 are separated by 151 nucleotides
GFF136 - FIG002842: hypothetical protein, at 125,925 to 126,626
GFF136
GFF137 - FIG003276: zinc-binding protein, at 126,636 to 126,827
GFF137
GFF138 - Aldo-keto reductase, at 126,981 to 127,115
GFF138
GFF139 - Mutator mutT protein (7,8-dihydro-8-oxoguanine-triphosphatase) (EC 3.6.1.-), at 127,149 to 127,544
GFF139
GFF140 - Protein export cytoplasm protein SecA ATPase RNA helicase (TC 3.A.5.1.1), at 127,696 to 130,401
GFF140
Position (kb) 127
128 Strain fitness (log2 ratio) -1
0
1
2 at 126.167 kb on - strand, within GFF136 at 126.167 kb on - strand, within GFF136 at 126.261 kb on - strand, within GFF136 at 126.302 kb on + strand, within GFF136 at 126.303 kb on - strand, within GFF136 at 126.303 kb on - strand, within GFF136 at 126.491 kb on + strand, within GFF136 at 126.492 kb on - strand, within GFF136 at 126.537 kb on - strand, within GFF136 at 126.537 kb on - strand, within GFF136 at 126.537 kb on - strand, within GFF136 at 126.655 kb on + strand at 126.924 kb on + strand at 126.944 kb on + strand at 126.949 kb on + strand at 127.105 kb on + strand at 127.117 kb on - strand at 127.305 kb on - strand, within GFF139 at 127.364 kb on + strand, within GFF139 at 127.383 kb on + strand, within GFF139 at 127.383 kb on + strand, within GFF139 at 127.384 kb on - strand, within GFF139 at 127.665 kb on + strand at 127.673 kb on - strand at 127.910 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 126,167 - GFF136 0.34 -0.3 126,167 - GFF136 0.34 -0.1 126,261 - GFF136 0.48 +0.6 126,302 + GFF136 0.54 +0.4 126,303 - GFF136 0.54 -0.1 126,303 - GFF136 0.54 -0.0 126,491 + GFF136 0.81 +0.4 126,492 - GFF136 0.81 +2.5 126,537 - GFF136 0.87 +0.5 126,537 - GFF136 0.87 +1.1 126,537 - GFF136 0.87 +0.5 126,655 + +0.3 126,924 + -0.5 126,944 + +0.3 126,949 + -0.0 127,105 + +0.3 127,117 - +1.9 127,305 - GFF139 0.39 +0.6 127,364 + GFF139 0.54 +0.2 127,383 + GFF139 0.59 +0.6 127,383 + GFF139 0.59 +0.0 127,384 - GFF139 0.59 +2.7 127,665 + +0.2 127,673 - +0.0 127,910 - -0.2
Or see this region's nucleotide sequence