Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1353

Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1352 and GFF1353 are separated by 3 nucleotidesGFF1353 and GFF1354 overlap by 25 nucleotides GFF1352 - Isocitrate lyase (EC 4.1.3.1), at 3,800 to 5,104 GFF1352 GFF1353 - hypothetical protein, at 5,108 to 5,230 GFF1353 GFF1354 - Isocitrate dehydrogenase phosphatase (EC 2.7.11.5)/kinase (EC 3.1.3.-), at 5,206 to 6,957 GFF1354 Position (kb) 5 6Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 4.116 kb on - strand, within GFF1352at 4.247 kb on + strand, within GFF1352at 4.555 kb on + strand, within GFF1352at 4.586 kb on + strand, within GFF1352at 4.673 kb on - strand, within GFF1352at 4.673 kb on - strand, within GFF1352at 4.673 kb on - strand, within GFF1352at 4.747 kb on + strand, within GFF1352at 4.839 kb on - strand, within GFF1352at 4.846 kb on + strand, within GFF1352at 4.904 kb on + strand, within GFF1352at 4.932 kb on - strand, within GFF1352at 5.019 kb on - strandat 5.019 kb on - strandat 5.148 kb on + strand, within GFF1353at 5.406 kb on + strand, within GFF1354at 5.407 kb on - strand, within GFF1354at 5.407 kb on - strand, within GFF1354at 5.710 kb on - strand, within GFF1354at 5.730 kb on + strand, within GFF1354at 5.731 kb on - strand, within GFF1354at 5.809 kb on + strand, within GFF1354at 5.844 kb on - strand, within GFF1354at 5.917 kb on - strand, within GFF1354at 5.977 kb on - strand, within GFF1354at 5.977 kb on - strand, within GFF1354at 5.984 kb on - strand, within GFF1354at 6.056 kb on - strand, within GFF1354at 6.058 kb on - strand, within GFF1354at 6.110 kb on + strand, within GFF1354at 6.111 kb on - strand, within GFF1354

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
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4,116 - GFF1352 0.24 -0.5
4,247 + GFF1352 0.34 +0.1
4,555 + GFF1352 0.58 +0.5
4,586 + GFF1352 0.60 -1.7
4,673 - GFF1352 0.67 -4.3
4,673 - GFF1352 0.67 +0.8
4,673 - GFF1352 0.67 -0.7
4,747 + GFF1352 0.73 +0.4
4,839 - GFF1352 0.80 +0.0
4,846 + GFF1352 0.80 -1.4
4,904 + GFF1352 0.85 -0.1
4,932 - GFF1352 0.87 +1.8
5,019 - -1.5
5,019 - -0.6
5,148 + GFF1353 0.33 +0.8
5,406 + GFF1354 0.11 +0.9
5,407 - GFF1354 0.11 -0.7
5,407 - GFF1354 0.11 -0.3
5,710 - GFF1354 0.29 -0.2
5,730 + GFF1354 0.30 -1.2
5,731 - GFF1354 0.30 +0.1
5,809 + GFF1354 0.34 +0.6
5,844 - GFF1354 0.36 -2.5
5,917 - GFF1354 0.41 +1.5
5,977 - GFF1354 0.44 -0.8
5,977 - GFF1354 0.44 +0.7
5,984 - GFF1354 0.44 +1.2
6,056 - GFF1354 0.49 -0.4
6,058 - GFF1354 0.49 -0.3
6,110 + GFF1354 0.52 -2.6
6,111 - GFF1354 0.52 +0.5

Or see this region's nucleotide sequence