Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1300

Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1299 and GFF1300 are separated by 102 nucleotidesGFF1300 and GFF1301 overlap by 4 nucleotides GFF1299 - N-acetylglutamate synthase (EC 2.3.1.1), at 1,650 to 2,981 GFF1299 GFF1300 - Exodeoxyribonuclease V alpha chain (EC 3.1.11.5), at 3,084 to 4,919 GFF1300 GFF1301 - Exodeoxyribonuclease V beta chain (EC 3.1.11.5), at 4,916 to 8,461 GFF1301 Position (kb) 3 4 5Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 2.376 kb on + strand, within GFF1299at 2.660 kb on + strand, within GFF1299at 2.667 kb on - strand, within GFF1299at 2.678 kb on + strand, within GFF1299at 2.902 kb on + strandat 2.903 kb on - strandat 3.017 kb on + strandat 3.086 kb on - strandat 3.111 kb on + strandat 3.112 kb on - strandat 3.221 kb on - strandat 3.306 kb on + strand, within GFF1300at 3.326 kb on + strand, within GFF1300at 3.490 kb on + strand, within GFF1300at 3.710 kb on - strand, within GFF1300at 3.742 kb on - strand, within GFF1300at 3.744 kb on - strand, within GFF1300at 3.798 kb on - strand, within GFF1300at 3.934 kb on - strand, within GFF1300at 4.391 kb on + strand, within GFF1300at 4.488 kb on + strand, within GFF1300at 4.525 kb on - strand, within GFF1300at 4.598 kb on - strand, within GFF1300at 4.719 kb on + strand, within GFF1300

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
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2,376 + GFF1299 0.55 -0.5
2,660 + GFF1299 0.76 -1.6
2,667 - GFF1299 0.76 +0.8
2,678 + GFF1299 0.77 -1.2
2,902 + -0.4
2,903 - +0.0
3,017 + +0.1
3,086 - -0.6
3,111 + +2.4
3,112 - -1.8
3,221 - -1.6
3,306 + GFF1300 0.12 -1.0
3,326 + GFF1300 0.13 -3.2
3,490 + GFF1300 0.22 -0.0
3,710 - GFF1300 0.34 -4.5
3,742 - GFF1300 0.36 -0.1
3,744 - GFF1300 0.36 -2.7
3,798 - GFF1300 0.39 -1.2
3,934 - GFF1300 0.46 -1.6
4,391 + GFF1300 0.71 -0.5
4,488 + GFF1300 0.76 -1.3
4,525 - GFF1300 0.78 -1.9
4,598 - GFF1300 0.82 -3.7
4,719 + GFF1300 0.89 -2.8

Or see this region's nucleotide sequence