Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1255 and GFF1256 are separated by 498 nucleotides GFF1256 and GFF1257 overlap by 1 nucleotides
GFF1255 - Transposase, at 82,678 to 83,535
GFF1255
GFF1256 - Chromosome (plasmid) partitioning protein ParA, at 84,034 to 85,239
GFF1256
GFF1257 - Chromosome (plasmid) partitioning protein ParB, at 85,239 to 86,213
GFF1257
Position (kb)
84
85
86 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 83.071 kb on - strand, within GFF1255 at 83.169 kb on - strand, within GFF1255 at 83.169 kb on - strand, within GFF1255 at 83.249 kb on + strand, within GFF1255 at 83.250 kb on - strand, within GFF1255 at 83.250 kb on - strand, within GFF1255 at 83.324 kb on + strand, within GFF1255 at 83.346 kb on + strand, within GFF1255 at 83.347 kb on - strand, within GFF1255 at 83.388 kb on + strand, within GFF1255 at 83.389 kb on - strand, within GFF1255 at 83.389 kb on - strand, within GFF1255 at 83.421 kb on - strand, within GFF1255 at 83.438 kb on + strand, within GFF1255 at 83.536 kb on - strand at 83.654 kb on - strand at 83.755 kb on + strand at 83.791 kb on - strand at 83.797 kb on + strand at 83.797 kb on + strand at 83.797 kb on + strand at 83.910 kb on + strand at 83.911 kb on - strand at 83.930 kb on - strand at 84.135 kb on + strand at 84.172 kb on + strand, within GFF1256 at 84.173 kb on - strand, within GFF1256 at 84.173 kb on - strand, within GFF1256 at 84.267 kb on + strand, within GFF1256 at 84.365 kb on + strand, within GFF1256 at 84.371 kb on + strand, within GFF1256 at 84.376 kb on - strand, within GFF1256 at 84.593 kb on + strand, within GFF1256 at 84.717 kb on + strand, within GFF1256 at 84.774 kb on + strand, within GFF1256 at 85.327 kb on + strand at 85.594 kb on + strand, within GFF1257 at 85.662 kb on - strand, within GFF1257 at 85.763 kb on + strand, within GFF1257 at 85.763 kb on + strand, within GFF1257
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 83,071 - GFF1255 0.46 -2.0 83,169 - GFF1255 0.57 -0.7 83,169 - GFF1255 0.57 +0.3 83,249 + GFF1255 0.67 -2.4 83,250 - GFF1255 0.67 +0.2 83,250 - GFF1255 0.67 +0.3 83,324 + GFF1255 0.75 -2.7 83,346 + GFF1255 0.78 +0.9 83,347 - GFF1255 0.78 +0.6 83,388 + GFF1255 0.83 -0.6 83,389 - GFF1255 0.83 +1.9 83,389 - GFF1255 0.83 -1.7 83,421 - GFF1255 0.87 +0.4 83,438 + GFF1255 0.89 +1.0 83,536 - +0.4 83,654 - +0.8 83,755 + +0.5 83,791 - +0.4 83,797 + -1.3 83,797 + +1.2 83,797 + +1.8 83,910 + -1.3 83,911 - +2.1 83,930 - -0.8 84,135 + -0.5 84,172 + GFF1256 0.11 -0.1 84,173 - GFF1256 0.12 -0.5 84,173 - GFF1256 0.12 -1.6 84,267 + GFF1256 0.19 -0.8 84,365 + GFF1256 0.27 -2.2 84,371 + GFF1256 0.28 -1.3 84,376 - GFF1256 0.28 -0.5 84,593 + GFF1256 0.46 -0.8 84,717 + GFF1256 0.57 -1.6 84,774 + GFF1256 0.61 +0.1 85,327 + -1.9 85,594 + GFF1257 0.36 -1.6 85,662 - GFF1257 0.43 +1.5 85,763 + GFF1257 0.54 -2.1 85,763 + GFF1257 0.54 -0.1
Or see this region's nucleotide sequence