Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1225 and GFF1226 are separated by 34 nucleotides GFF1226 and GFF1227 are separated by 8 nucleotides GFF1227 and GFF1228 are separated by 186 nucleotides GFF1228 and GFF1229 overlap by 7 nucleotides
GFF1225 - putative cytoplasmic protein, at 61,133 to 61,654
GFF1225
GFF1226 - Resolvase, at 61,689 to 62,471
GFF1226
GFF1227 - Putative diguanylate cyclase/phosphodiesterase, at 62,480 to 63,031
GFF1227
GFF1228 - Orf10, at 63,218 to 63,706
GFF1228
GFF1229 - Putative inner membrane protein, at 63,700 to 64,185
GFF1229
Position (kb) 62
63
64 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 61.608 kb on - strand at 61.834 kb on + strand, within GFF1226 at 61.835 kb on - strand, within GFF1226 at 61.851 kb on + strand, within GFF1226 at 61.851 kb on + strand, within GFF1226 at 61.852 kb on - strand, within GFF1226 at 61.852 kb on - strand, within GFF1226 at 62.079 kb on + strand, within GFF1226 at 62.080 kb on - strand, within GFF1226 at 62.270 kb on + strand, within GFF1226 at 62.271 kb on - strand, within GFF1226 at 62.287 kb on - strand, within GFF1226 at 62.373 kb on + strand, within GFF1226 at 62.469 kb on + strand at 62.531 kb on - strand at 62.540 kb on + strand, within GFF1227 at 62.541 kb on - strand, within GFF1227 at 62.782 kb on + strand, within GFF1227 at 62.904 kb on + strand, within GFF1227 at 63.030 kb on - strand at 63.104 kb on + strand at 63.191 kb on + strand at 63.218 kb on - strand at 63.428 kb on + strand, within GFF1228 at 63.428 kb on + strand, within GFF1228 at 63.428 kb on + strand, within GFF1228 at 63.437 kb on - strand, within GFF1228 at 63.599 kb on + strand, within GFF1228 at 63.600 kb on - strand, within GFF1228 at 63.650 kb on - strand, within GFF1228
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 61,608 - -1.3 61,834 + GFF1226 0.19 -0.5 61,835 - GFF1226 0.19 +0.5 61,851 + GFF1226 0.21 -1.6 61,851 + GFF1226 0.21 +1.2 61,852 - GFF1226 0.21 +0.3 61,852 - GFF1226 0.21 -1.3 62,079 + GFF1226 0.50 +0.7 62,080 - GFF1226 0.50 -0.3 62,270 + GFF1226 0.74 +0.9 62,271 - GFF1226 0.74 -0.2 62,287 - GFF1226 0.76 -2.1 62,373 + GFF1226 0.87 -0.3 62,469 + +0.3 62,531 - +1.9 62,540 + GFF1227 0.11 -0.5 62,541 - GFF1227 0.11 +0.3 62,782 + GFF1227 0.55 +3.3 62,904 + GFF1227 0.77 +0.0 63,030 - -2.1 63,104 + -0.1 63,191 + -0.1 63,218 - +0.1 63,428 + GFF1228 0.43 +0.4 63,428 + GFF1228 0.43 -1.8 63,428 + GFF1228 0.43 +1.1 63,437 - GFF1228 0.45 -0.2 63,599 + GFF1228 0.78 -0.3 63,600 - GFF1228 0.78 +2.0 63,650 - GFF1228 0.88 -0.1
Or see this region's nucleotide sequence