Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1118 and GFF1119 are separated by 162 nucleotides GFF1119 and GFF1120 are separated by 61 nucleotides
GFF1118 - Possible sulfatase, at 305,096 to 306,811
GFF1118
GFF1119 - Na+/H+ antiporter NhaA type, at 306,974 to 308,140
GFF1119
GFF1120 - Transcriptional activator NhaR, at 308,202 to 309,101
GFF1120
Position (kb) 306
307
308
309 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 306.047 kb on - strand, within GFF1118 at 306.060 kb on - strand, within GFF1118 at 306.060 kb on - strand, within GFF1118 at 306.084 kb on + strand, within GFF1118 at 306.186 kb on - strand, within GFF1118 at 306.299 kb on - strand, within GFF1118 at 306.331 kb on + strand, within GFF1118 at 306.332 kb on - strand, within GFF1118 at 306.334 kb on - strand, within GFF1118 at 306.334 kb on - strand, within GFF1118 at 306.348 kb on + strand, within GFF1118 at 306.361 kb on + strand, within GFF1118 at 306.366 kb on - strand, within GFF1118 at 306.440 kb on - strand, within GFF1118 at 306.473 kb on + strand, within GFF1118 at 306.619 kb on + strand, within GFF1118 at 306.769 kb on + strand at 306.770 kb on - strand at 306.788 kb on + strand at 306.789 kb on - strand at 306.790 kb on + strand at 306.804 kb on - strand at 306.854 kb on + strand at 306.896 kb on + strand at 306.929 kb on + strand at 306.997 kb on + strand at 307.595 kb on + strand, within GFF1119 at 307.597 kb on + strand, within GFF1119 at 307.597 kb on + strand, within GFF1119 at 307.598 kb on - strand, within GFF1119 at 307.598 kb on - strand, within GFF1119 at 307.598 kb on - strand, within GFF1119 at 307.946 kb on + strand, within GFF1119 at 307.946 kb on + strand, within GFF1119 at 307.947 kb on - strand, within GFF1119 at 308.045 kb on - strand at 308.105 kb on - strand at 308.217 kb on - strand at 308.223 kb on + strand at 308.223 kb on + strand at 308.236 kb on - strand at 308.253 kb on + strand at 308.253 kb on + strand at 308.366 kb on + strand, within GFF1120 at 308.367 kb on - strand, within GFF1120 at 308.432 kb on + strand, within GFF1120 at 308.573 kb on - strand, within GFF1120 at 308.645 kb on + strand, within GFF1120 at 308.887 kb on + strand, within GFF1120 at 309.009 kb on + strand, within GFF1120 at 309.009 kb on + strand, within GFF1120 at 309.090 kb on - strand at 309.099 kb on + strand at 309.100 kb on - strand at 309.100 kb on - strand at 309.106 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 306,047 - GFF1118 0.55 +2.9 306,060 - GFF1118 0.56 -1.3 306,060 - GFF1118 0.56 +1.1 306,084 + GFF1118 0.58 -0.9 306,186 - GFF1118 0.64 +1.4 306,299 - GFF1118 0.70 -1.9 306,331 + GFF1118 0.72 +1.2 306,332 - GFF1118 0.72 +0.6 306,334 - GFF1118 0.72 +0.1 306,334 - GFF1118 0.72 +0.6 306,348 + GFF1118 0.73 +0.4 306,361 + GFF1118 0.74 +0.6 306,366 - GFF1118 0.74 -0.2 306,440 - GFF1118 0.78 +0.2 306,473 + GFF1118 0.80 +1.3 306,619 + GFF1118 0.89 +0.8 306,769 + +0.7 306,770 - -0.0 306,788 + +0.6 306,789 - +0.3 306,790 + +0.2 306,804 - -0.1 306,854 + +0.4 306,896 + +0.6 306,929 + +0.3 306,997 + -0.3 307,595 + GFF1119 0.53 -1.4 307,597 + GFF1119 0.53 -2.4 307,597 + GFF1119 0.53 -0.4 307,598 - GFF1119 0.53 -2.7 307,598 - GFF1119 0.53 -2.3 307,598 - GFF1119 0.53 -4.4 307,946 + GFF1119 0.83 -2.3 307,946 + GFF1119 0.83 -1.5 307,947 - GFF1119 0.83 -0.1 308,045 - +0.6 308,105 - -4.1 308,217 - -1.1 308,223 + +0.9 308,223 + -1.2 308,236 - +0.2 308,253 + +2.2 308,253 + -0.5 308,366 + GFF1120 0.18 +0.0 308,367 - GFF1120 0.18 +0.1 308,432 + GFF1120 0.26 +1.2 308,573 - GFF1120 0.41 +0.1 308,645 + GFF1120 0.49 +0.8 308,887 + GFF1120 0.76 +0.5 309,009 + GFF1120 0.90 -0.8 309,009 + GFF1120 0.90 -2.6 309,090 - +0.6 309,099 + +0.7 309,100 - -1.1 309,100 - +2.3 309,106 - -0.8
Or see this region's nucleotide sequence