Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1117 and GFF1118 are separated by 126 nucleotides GFF1118 and GFF1119 are separated by 162 nucleotides
GFF1117 - FIG074102: hypothetical protein, at 303,722 to 304,969
GFF1117
GFF1118 - Possible sulfatase, at 305,096 to 306,811
GFF1118
GFF1119 - Na+/H+ antiporter NhaA type, at 306,974 to 308,140
GFF1119
Position (kb) 305
306
307 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 304.260 kb on + strand, within GFF1117 at 304.261 kb on - strand, within GFF1117 at 304.261 kb on - strand, within GFF1117 at 304.262 kb on + strand, within GFF1117 at 304.263 kb on - strand, within GFF1117 at 304.295 kb on - strand, within GFF1117 at 304.497 kb on + strand, within GFF1117 at 304.501 kb on - strand, within GFF1117 at 304.501 kb on - strand, within GFF1117 at 304.512 kb on + strand, within GFF1117 at 304.615 kb on + strand, within GFF1117 at 304.615 kb on + strand, within GFF1117 at 304.616 kb on - strand, within GFF1117 at 304.634 kb on + strand, within GFF1117 at 304.754 kb on - strand, within GFF1117 at 304.837 kb on - strand, within GFF1117 at 305.111 kb on - strand at 305.294 kb on + strand, within GFF1118 at 305.294 kb on + strand, within GFF1118 at 305.294 kb on + strand, within GFF1118 at 305.381 kb on + strand, within GFF1118 at 305.382 kb on - strand, within GFF1118 at 305.382 kb on - strand, within GFF1118 at 305.484 kb on - strand, within GFF1118 at 305.533 kb on - strand, within GFF1118 at 305.637 kb on + strand, within GFF1118 at 305.637 kb on + strand, within GFF1118 at 305.759 kb on + strand, within GFF1118 at 305.760 kb on - strand, within GFF1118 at 305.867 kb on + strand, within GFF1118 at 306.047 kb on - strand, within GFF1118 at 306.060 kb on - strand, within GFF1118 at 306.060 kb on - strand, within GFF1118 at 306.084 kb on + strand, within GFF1118 at 306.186 kb on - strand, within GFF1118 at 306.299 kb on - strand, within GFF1118 at 306.331 kb on + strand, within GFF1118 at 306.332 kb on - strand, within GFF1118 at 306.334 kb on - strand, within GFF1118 at 306.334 kb on - strand, within GFF1118 at 306.348 kb on + strand, within GFF1118 at 306.361 kb on + strand, within GFF1118 at 306.366 kb on - strand, within GFF1118 at 306.440 kb on - strand, within GFF1118 at 306.473 kb on + strand, within GFF1118 at 306.619 kb on + strand, within GFF1118 at 306.769 kb on + strand at 306.770 kb on - strand at 306.788 kb on + strand at 306.789 kb on - strand at 306.790 kb on + strand at 306.804 kb on - strand at 306.854 kb on + strand at 306.896 kb on + strand at 306.929 kb on + strand at 306.997 kb on + strand at 307.595 kb on + strand, within GFF1119 at 307.597 kb on + strand, within GFF1119 at 307.597 kb on + strand, within GFF1119 at 307.598 kb on - strand, within GFF1119 at 307.598 kb on - strand, within GFF1119 at 307.598 kb on - strand, within GFF1119
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 304,260 + GFF1117 0.43 +0.5 304,261 - GFF1117 0.43 -1.4 304,261 - GFF1117 0.43 +1.0 304,262 + GFF1117 0.43 +0.1 304,263 - GFF1117 0.43 -2.6 304,295 - GFF1117 0.46 +0.3 304,497 + GFF1117 0.62 -0.7 304,501 - GFF1117 0.62 -0.8 304,501 - GFF1117 0.62 +0.4 304,512 + GFF1117 0.63 -1.1 304,615 + GFF1117 0.72 +0.5 304,615 + GFF1117 0.72 +0.3 304,616 - GFF1117 0.72 +1.3 304,634 + GFF1117 0.73 -1.6 304,754 - GFF1117 0.83 +0.5 304,837 - GFF1117 0.89 -1.1 305,111 - -0.8 305,294 + GFF1118 0.12 -0.2 305,294 + GFF1118 0.12 -0.0 305,294 + GFF1118 0.12 -1.3 305,381 + GFF1118 0.17 +1.2 305,382 - GFF1118 0.17 +0.4 305,382 - GFF1118 0.17 +0.7 305,484 - GFF1118 0.23 +0.4 305,533 - GFF1118 0.25 -0.4 305,637 + GFF1118 0.32 +1.2 305,637 + GFF1118 0.32 +1.4 305,759 + GFF1118 0.39 -0.4 305,760 - GFF1118 0.39 +0.2 305,867 + GFF1118 0.45 -1.2 306,047 - GFF1118 0.55 +2.9 306,060 - GFF1118 0.56 -1.3 306,060 - GFF1118 0.56 +1.1 306,084 + GFF1118 0.58 -0.9 306,186 - GFF1118 0.64 +1.4 306,299 - GFF1118 0.70 -1.9 306,331 + GFF1118 0.72 +1.2 306,332 - GFF1118 0.72 +0.6 306,334 - GFF1118 0.72 +0.1 306,334 - GFF1118 0.72 +0.6 306,348 + GFF1118 0.73 +0.4 306,361 + GFF1118 0.74 +0.6 306,366 - GFF1118 0.74 -0.2 306,440 - GFF1118 0.78 +0.2 306,473 + GFF1118 0.80 +1.3 306,619 + GFF1118 0.89 +0.8 306,769 + +0.7 306,770 - -0.0 306,788 + +0.6 306,789 - +0.3 306,790 + +0.2 306,804 - -0.1 306,854 + +0.4 306,896 + +0.6 306,929 + +0.3 306,997 + -0.3 307,595 + GFF1119 0.53 -1.4 307,597 + GFF1119 0.53 -2.4 307,597 + GFF1119 0.53 -0.4 307,598 - GFF1119 0.53 -2.7 307,598 - GFF1119 0.53 -2.3 307,598 - GFF1119 0.53 -4.4
Or see this region's nucleotide sequence