Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1046 and GFF1047 are separated by 37 nucleotides GFF1047 and GFF1048 overlap by 4 nucleotides GFF1048 and GFF1049 are separated by 13 nucleotides GFF1049 and GFF1050 are separated by 77 nucleotides
GFF1046 - Purine nucleoside phosphorylase (EC 2.4.2.1), at 228,493 to 229,212
GFF1046
GFF1047 - Beta-fimbriae probable major subunit, at 229,250 to 229,822
GFF1047
GFF1048 - Beta-fimbriae usher protein, at 229,819 to 232,227
GFF1048
GFF1049 - Beta-fimbriae chaperone protein, at 232,241 to 232,948
GFF1049
GFF1050 - Beta-fimbriae probable major subunit, at 233,026 to 233,715
GFF1050
Position (kb)
229
230
231
232
233 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 228.855 kb on + strand, within GFF1046 at 228.974 kb on - strand, within GFF1046 at 229.028 kb on + strand, within GFF1046 at 229.047 kb on + strand, within GFF1046 at 229.117 kb on - strand, within GFF1046 at 229.204 kb on - strand at 229.286 kb on + strand at 229.286 kb on + strand at 229.286 kb on + strand at 229.287 kb on - strand at 229.287 kb on - strand at 229.341 kb on - strand, within GFF1047 at 229.385 kb on - strand, within GFF1047 at 229.388 kb on - strand, within GFF1047 at 229.411 kb on + strand, within GFF1047 at 229.411 kb on + strand, within GFF1047 at 229.419 kb on - strand, within GFF1047 at 229.489 kb on - strand, within GFF1047 at 229.502 kb on + strand, within GFF1047 at 229.503 kb on - strand, within GFF1047 at 229.597 kb on + strand, within GFF1047 at 229.597 kb on + strand, within GFF1047 at 229.767 kb on - strand at 229.781 kb on - strand at 229.787 kb on + strand at 229.787 kb on + strand at 229.789 kb on + strand at 229.789 kb on + strand at 229.928 kb on - strand at 229.992 kb on - strand at 229.996 kb on - strand at 230.009 kb on - strand at 230.009 kb on - strand at 230.053 kb on + strand at 230.053 kb on + strand at 230.179 kb on - strand, within GFF1048 at 230.317 kb on - strand, within GFF1048 at 230.329 kb on - strand, within GFF1048 at 230.330 kb on + strand, within GFF1048 at 230.514 kb on - strand, within GFF1048 at 230.544 kb on + strand, within GFF1048 at 230.586 kb on + strand, within GFF1048 at 230.588 kb on + strand, within GFF1048 at 230.589 kb on - strand, within GFF1048 at 230.612 kb on - strand, within GFF1048 at 230.612 kb on - strand, within GFF1048 at 230.641 kb on - strand, within GFF1048 at 230.653 kb on - strand, within GFF1048 at 230.749 kb on - strand, within GFF1048 at 230.790 kb on - strand, within GFF1048 at 230.808 kb on + strand, within GFF1048 at 230.809 kb on - strand, within GFF1048 at 230.835 kb on + strand, within GFF1048 at 230.865 kb on - strand, within GFF1048 at 230.938 kb on - strand, within GFF1048 at 230.969 kb on - strand, within GFF1048 at 231.046 kb on - strand, within GFF1048 at 231.086 kb on + strand, within GFF1048 at 231.196 kb on + strand, within GFF1048 at 231.197 kb on - strand, within GFF1048 at 231.197 kb on - strand, within GFF1048 at 231.197 kb on - strand, within GFF1048 at 231.218 kb on - strand, within GFF1048 at 231.310 kb on - strand, within GFF1048 at 231.352 kb on - strand, within GFF1048 at 231.435 kb on + strand, within GFF1048 at 231.449 kb on - strand, within GFF1048 at 231.486 kb on + strand, within GFF1048 at 231.520 kb on + strand, within GFF1048 at 231.594 kb on + strand, within GFF1048 at 231.594 kb on + strand, within GFF1048 at 231.595 kb on - strand, within GFF1048 at 231.595 kb on - strand, within GFF1048 at 231.595 kb on - strand, within GFF1048 at 231.595 kb on - strand, within GFF1048 at 231.598 kb on + strand, within GFF1048 at 231.599 kb on - strand, within GFF1048 at 231.599 kb on - strand, within GFF1048 at 231.627 kb on + strand, within GFF1048 at 231.673 kb on + strand, within GFF1048 at 231.751 kb on - strand, within GFF1048 at 231.757 kb on - strand, within GFF1048 at 231.799 kb on + strand, within GFF1048 at 231.802 kb on + strand, within GFF1048 at 231.808 kb on - strand, within GFF1048 at 231.870 kb on + strand, within GFF1048 at 231.896 kb on - strand, within GFF1048 at 232.058 kb on - strand at 232.202 kb on + strand at 232.202 kb on + strand at 232.203 kb on - strand at 232.203 kb on - strand at 232.203 kb on - strand at 232.203 kb on - strand at 232.205 kb on - strand at 232.340 kb on - strand, within GFF1049 at 232.511 kb on - strand, within GFF1049 at 232.541 kb on + strand, within GFF1049 at 232.630 kb on - strand, within GFF1049 at 232.673 kb on + strand, within GFF1049 at 232.766 kb on - strand, within GFF1049 at 233.038 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 228,855 + GFF1046 0.50 -1.0 228,974 - GFF1046 0.67 +0.4 229,028 + GFF1046 0.74 -1.0 229,047 + GFF1046 0.77 -1.0 229,117 - GFF1046 0.87 -1.3 229,204 - +0.6 229,286 + +0.1 229,286 + +0.7 229,286 + +0.3 229,287 - -0.1 229,287 - +1.4 229,341 - GFF1047 0.16 +1.5 229,385 - GFF1047 0.24 -0.1 229,388 - GFF1047 0.24 -1.0 229,411 + GFF1047 0.28 +0.2 229,411 + GFF1047 0.28 +1.0 229,419 - GFF1047 0.29 +0.2 229,489 - GFF1047 0.42 -0.3 229,502 + GFF1047 0.44 +0.1 229,503 - GFF1047 0.44 -1.7 229,597 + GFF1047 0.61 +0.2 229,597 + GFF1047 0.61 +0.9 229,767 - +0.3 229,781 - -0.4 229,787 + -1.2 229,787 + -1.0 229,789 + +0.2 229,789 + -1.0 229,928 - -1.0 229,992 - +1.3 229,996 - +2.2 230,009 - +0.0 230,009 - +0.4 230,053 + -0.2 230,053 + +1.8 230,179 - GFF1048 0.15 -1.8 230,317 - GFF1048 0.21 -0.5 230,329 - GFF1048 0.21 +0.0 230,330 + GFF1048 0.21 +0.1 230,514 - GFF1048 0.29 +1.0 230,544 + GFF1048 0.30 +2.2 230,586 + GFF1048 0.32 +1.3 230,588 + GFF1048 0.32 -2.2 230,589 - GFF1048 0.32 -0.5 230,612 - GFF1048 0.33 -0.4 230,612 - GFF1048 0.33 +1.0 230,641 - GFF1048 0.34 +0.3 230,653 - GFF1048 0.35 +0.5 230,749 - GFF1048 0.39 +0.5 230,790 - GFF1048 0.40 -2.8 230,808 + GFF1048 0.41 +0.6 230,809 - GFF1048 0.41 -0.5 230,835 + GFF1048 0.42 +1.5 230,865 - GFF1048 0.43 +3.0 230,938 - GFF1048 0.46 +0.8 230,969 - GFF1048 0.48 -0.3 231,046 - GFF1048 0.51 +0.4 231,086 + GFF1048 0.53 +1.3 231,196 + GFF1048 0.57 +0.6 231,197 - GFF1048 0.57 -1.6 231,197 - GFF1048 0.57 +0.7 231,197 - GFF1048 0.57 +0.8 231,218 - GFF1048 0.58 +0.9 231,310 - GFF1048 0.62 -0.8 231,352 - GFF1048 0.64 +0.4 231,435 + GFF1048 0.67 -0.1 231,449 - GFF1048 0.68 -0.5 231,486 + GFF1048 0.69 +0.1 231,520 + GFF1048 0.71 +1.0 231,594 + GFF1048 0.74 +0.1 231,594 + GFF1048 0.74 +0.7 231,595 - GFF1048 0.74 -1.4 231,595 - GFF1048 0.74 +0.1 231,595 - GFF1048 0.74 -0.1 231,595 - GFF1048 0.74 +1.0 231,598 + GFF1048 0.74 -0.1 231,599 - GFF1048 0.74 -0.9 231,599 - GFF1048 0.74 -0.3 231,627 + GFF1048 0.75 -1.8 231,673 + GFF1048 0.77 -0.3 231,751 - GFF1048 0.80 +0.1 231,757 - GFF1048 0.80 -1.8 231,799 + GFF1048 0.82 +0.2 231,802 + GFF1048 0.82 +0.9 231,808 - GFF1048 0.83 +0.7 231,870 + GFF1048 0.85 +2.7 231,896 - GFF1048 0.86 +1.2 232,058 - -0.8 232,202 + -0.4 232,202 + +0.4 232,203 - +0.8 232,203 - +0.3 232,203 - -0.2 232,203 - +0.0 232,205 - -0.1 232,340 - GFF1049 0.14 -2.1 232,511 - GFF1049 0.38 -1.4 232,541 + GFF1049 0.42 +0.1 232,630 - GFF1049 0.55 +0.4 232,673 + GFF1049 0.61 -0.7 232,766 - GFF1049 0.74 -1.4 233,038 - -0.0
Or see this region's nucleotide sequence