Experiment: LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1037 and GFF1038 are separated by 128 nucleotides GFF1038 and GFF1039 are separated by 123 nucleotides GFF1039 and GFF1040 overlap by 4 nucleotides GFF1040 and GFF1041 are separated by 32 nucleotides
GFF1037 - Osmotically inducible protein OsmY, at 219,231 to 219,848
GFF1037
GFF1038 - FIG01200701: possible membrane protein, at 219,977 to 220,138
GFF1038
GFF1039 - FIG00638703: hypothetical protein, at 220,262 to 221,335
GFF1039
GFF1040 - Putative deoxyribonuclease YjjV, at 221,332 to 222,105
GFF1040
GFF1041 - radical activating enzyme, at 222,138 to 222,872
GFF1041
Position (kb) 220
221
222 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 219.381 kb on - strand, within GFF1037 at 219.462 kb on - strand, within GFF1037 at 219.614 kb on + strand, within GFF1037 at 219.846 kb on + strand at 219.941 kb on + strand at 219.955 kb on - strand at 219.958 kb on + strand at 219.958 kb on + strand at 219.977 kb on - strand at 219.977 kb on - strand at 219.995 kb on - strand, within GFF1038 at 220.015 kb on - strand, within GFF1038 at 220.052 kb on - strand, within GFF1038 at 220.157 kb on + strand at 220.158 kb on - strand at 220.220 kb on - strand at 220.295 kb on - strand at 220.400 kb on - strand, within GFF1039 at 220.415 kb on - strand, within GFF1039 at 220.517 kb on - strand, within GFF1039 at 220.664 kb on + strand, within GFF1039 at 220.664 kb on + strand, within GFF1039 at 220.665 kb on - strand, within GFF1039 at 220.666 kb on + strand, within GFF1039 at 220.667 kb on - strand, within GFF1039 at 220.820 kb on - strand, within GFF1039 at 220.901 kb on + strand, within GFF1039 at 220.901 kb on + strand, within GFF1039 at 220.904 kb on + strand, within GFF1039 at 221.148 kb on - strand, within GFF1039 at 221.203 kb on + strand, within GFF1039 at 221.203 kb on + strand, within GFF1039 at 221.203 kb on + strand, within GFF1039 at 221.346 kb on + strand at 221.352 kb on + strand at 221.382 kb on + strand at 221.959 kb on - strand, within GFF1040 at 222.073 kb on + strand at 222.074 kb on - strand at 222.088 kb on - strand at 222.140 kb on - strand at 222.145 kb on + strand at 222.145 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with ES18h1_phage 0.00075 MOI remove 219,381 - GFF1037 0.24 +0.1 219,462 - GFF1037 0.37 -0.5 219,614 + GFF1037 0.62 +0.5 219,846 + +0.9 219,941 + +0.2 219,955 - -0.5 219,958 + -2.2 219,958 + +0.5 219,977 - -0.1 219,977 - +0.8 219,995 - GFF1038 0.11 +0.1 220,015 - GFF1038 0.23 -0.8 220,052 - GFF1038 0.46 -0.8 220,157 + +1.6 220,158 - +0.1 220,220 - +0.6 220,295 - -1.4 220,400 - GFF1039 0.13 -3.0 220,415 - GFF1039 0.14 -0.1 220,517 - GFF1039 0.24 +0.5 220,664 + GFF1039 0.37 -2.6 220,664 + GFF1039 0.37 +0.1 220,665 - GFF1039 0.38 -0.4 220,666 + GFF1039 0.38 -1.6 220,667 - GFF1039 0.38 -0.2 220,820 - GFF1039 0.52 -0.8 220,901 + GFF1039 0.59 +0.7 220,901 + GFF1039 0.59 +0.1 220,904 + GFF1039 0.60 -4.3 221,148 - GFF1039 0.82 +1.3 221,203 + GFF1039 0.88 +1.1 221,203 + GFF1039 0.88 -0.1 221,203 + GFF1039 0.88 +1.0 221,346 + +0.3 221,352 + -0.3 221,382 + +1.4 221,959 - GFF1040 0.81 -1.9 222,073 + -1.3 222,074 - +2.4 222,088 - +0.3 222,140 - +0.8 222,145 + +0.7 222,145 + -2.1
Or see this region's nucleotide sequence