Experiment: R2A with Phosphomycin 50 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0306 and Atu0307 are separated by 11 nucleotides Atu0307 and Atu0308 are separated by 10 nucleotides Atu0308 and Atu0309 are separated by 109 nucleotides Atu0309 and Atu0310 are separated by 242 nucleotides
Atu0306: Atu0306 - ABC transporter, membrane spanning protein (sn-Glycerol-3-phosphate), at 298,594 to 299,475
Atu0306
Atu0307: Atu0307 - ABC transporter, membrane spanning protein (sn-Glycerol-3-phosphate), at 299,487 to 300,329
Atu0307
Atu0308: Atu0308 - ABC transporter, nucleotide binding/ATPase protein (sn-Glycerol-3-phosphate), at 300,340 to 301,440
Atu0308
Atu0309: Atu0309 - hypothetical protein, at 301,550 to 302,062
Atu0309
Atu0310: Atu0310 - glutathione synthetase, at 302,305 to 303,264
Atu0310
Position (kb) 300
301
302 Strain fitness (log2 ratio) -2
-1
0
1
2 at 299.344 kb on + strand, within Atu0306 at 299.439 kb on - strand at 299.492 kb on - strand at 299.502 kb on + strand at 299.545 kb on - strand at 299.574 kb on + strand, within Atu0307 at 299.622 kb on - strand, within Atu0307 at 299.694 kb on + strand, within Atu0307 at 299.702 kb on - strand, within Atu0307 at 299.817 kb on + strand, within Atu0307 at 299.848 kb on - strand, within Atu0307 at 299.880 kb on - strand, within Atu0307 at 300.004 kb on - strand, within Atu0307 at 300.062 kb on + strand, within Atu0307 at 300.070 kb on - strand, within Atu0307 at 300.109 kb on + strand, within Atu0307 at 300.109 kb on + strand, within Atu0307 at 300.117 kb on - strand, within Atu0307 at 300.117 kb on - strand, within Atu0307 at 300.122 kb on + strand, within Atu0307 at 300.122 kb on + strand, within Atu0307 at 300.122 kb on + strand, within Atu0307 at 300.130 kb on - strand, within Atu0307 at 300.147 kb on + strand, within Atu0307 at 300.175 kb on + strand, within Atu0307 at 300.195 kb on + strand, within Atu0307 at 300.202 kb on - strand, within Atu0307 at 300.262 kb on + strand at 300.301 kb on + strand at 300.363 kb on + strand at 300.372 kb on + strand at 300.400 kb on - strand at 300.468 kb on + strand, within Atu0308 at 300.476 kb on - strand, within Atu0308 at 300.515 kb on + strand, within Atu0308 at 300.591 kb on - strand, within Atu0308 at 300.684 kb on + strand, within Atu0308 at 300.716 kb on - strand, within Atu0308 at 300.727 kb on - strand, within Atu0308 at 300.742 kb on + strand, within Atu0308 at 300.742 kb on + strand, within Atu0308 at 300.819 kb on + strand, within Atu0308 at 300.825 kb on + strand, within Atu0308 at 300.833 kb on - strand, within Atu0308 at 300.854 kb on - strand, within Atu0308 at 300.867 kb on + strand, within Atu0308 at 300.888 kb on + strand, within Atu0308 at 300.907 kb on - strand, within Atu0308 at 300.921 kb on - strand, within Atu0308 at 300.924 kb on + strand, within Atu0308 at 300.924 kb on + strand, within Atu0308 at 300.924 kb on + strand, within Atu0308 at 300.924 kb on + strand, within Atu0308 at 300.924 kb on + strand, within Atu0308 at 300.924 kb on + strand, within Atu0308 at 300.932 kb on - strand, within Atu0308 at 300.932 kb on - strand, within Atu0308 at 300.932 kb on - strand, within Atu0308 at 300.979 kb on + strand, within Atu0308 at 300.984 kb on + strand, within Atu0308 at 300.996 kb on + strand, within Atu0308 at 301.004 kb on - strand, within Atu0308 at 301.013 kb on + strand, within Atu0308 at 301.018 kb on + strand, within Atu0308 at 301.019 kb on - strand, within Atu0308 at 301.070 kb on + strand, within Atu0308 at 301.078 kb on - strand, within Atu0308 at 301.147 kb on - strand, within Atu0308 at 301.195 kb on + strand, within Atu0308 at 301.217 kb on + strand, within Atu0308 at 301.225 kb on - strand, within Atu0308 at 301.373 kb on + strand at 301.407 kb on - strand at 301.425 kb on + strand at 301.434 kb on + strand at 301.436 kb on + strand at 301.442 kb on - strand at 301.576 kb on + strand at 301.629 kb on + strand, within Atu0309 at 301.631 kb on + strand, within Atu0309 at 301.685 kb on + strand, within Atu0309 at 301.721 kb on + strand, within Atu0309 at 301.721 kb on + strand, within Atu0309 at 301.721 kb on + strand, within Atu0309 at 301.721 kb on + strand, within Atu0309 at 301.721 kb on + strand, within Atu0309 at 301.727 kb on - strand, within Atu0309 at 301.729 kb on - strand, within Atu0309 at 301.729 kb on - strand, within Atu0309 at 301.729 kb on - strand, within Atu0309 at 301.729 kb on - strand, within Atu0309 at 301.729 kb on - strand, within Atu0309 at 301.744 kb on + strand, within Atu0309 at 301.744 kb on + strand, within Atu0309 at 301.744 kb on + strand, within Atu0309 at 301.752 kb on - strand, within Atu0309 at 301.763 kb on + strand, within Atu0309 at 301.768 kb on + strand, within Atu0309 at 301.783 kb on - strand, within Atu0309 at 301.830 kb on - strand, within Atu0309 at 301.873 kb on - strand, within Atu0309 at 301.888 kb on + strand, within Atu0309 at 301.901 kb on - strand, within Atu0309 at 301.901 kb on - strand, within Atu0309 at 301.935 kb on + strand, within Atu0309 at 301.994 kb on + strand, within Atu0309 at 301.999 kb on + strand, within Atu0309 at 301.999 kb on + strand, within Atu0309 at 302.000 kb on + strand, within Atu0309 at 302.007 kb on - strand, within Atu0309 at 302.043 kb on + strand at 302.043 kb on + strand at 302.051 kb on - strand at 302.051 kb on - strand at 302.057 kb on + strand at 302.076 kb on - strand at 302.093 kb on + strand at 302.093 kb on + strand at 302.096 kb on - strand at 302.096 kb on - strand at 302.101 kb on + strand at 302.218 kb on + strand at 302.218 kb on + strand at 302.255 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Phosphomycin 50 mM remove 299,344 + Atu0306 0.85 +2.4 299,439 - -0.1 299,492 - +0.9 299,502 + -0.0 299,545 - -1.1 299,574 + Atu0307 0.10 +0.1 299,622 - Atu0307 0.16 -0.4 299,694 + Atu0307 0.25 -0.1 299,702 - Atu0307 0.26 +1.2 299,817 + Atu0307 0.39 -0.5 299,848 - Atu0307 0.43 -0.4 299,880 - Atu0307 0.47 -1.6 300,004 - Atu0307 0.61 -0.4 300,062 + Atu0307 0.68 +0.3 300,070 - Atu0307 0.69 +0.4 300,109 + Atu0307 0.74 -0.3 300,109 + Atu0307 0.74 +0.3 300,117 - Atu0307 0.75 -0.1 300,117 - Atu0307 0.75 -0.7 300,122 + Atu0307 0.75 +0.6 300,122 + Atu0307 0.75 +0.3 300,122 + Atu0307 0.75 -0.1 300,130 - Atu0307 0.76 -0.7 300,147 + Atu0307 0.78 +1.1 300,175 + Atu0307 0.82 +0.6 300,195 + Atu0307 0.84 -0.5 300,202 - Atu0307 0.85 +0.1 300,262 + -0.2 300,301 + +0.8 300,363 + +0.7 300,372 + +1.1 300,400 - +0.0 300,468 + Atu0308 0.12 -1.4 300,476 - Atu0308 0.12 -0.2 300,515 + Atu0308 0.16 -0.1 300,591 - Atu0308 0.23 -0.1 300,684 + Atu0308 0.31 -0.7 300,716 - Atu0308 0.34 -0.3 300,727 - Atu0308 0.35 +0.5 300,742 + Atu0308 0.37 +0.1 300,742 + Atu0308 0.37 -0.9 300,819 + Atu0308 0.44 -1.1 300,825 + Atu0308 0.44 +0.2 300,833 - Atu0308 0.45 -0.5 300,854 - Atu0308 0.47 -1.8 300,867 + Atu0308 0.48 +0.3 300,888 + Atu0308 0.50 -0.2 300,907 - Atu0308 0.51 -0.1 300,921 - Atu0308 0.53 -1.1 300,924 + Atu0308 0.53 -0.3 300,924 + Atu0308 0.53 -0.1 300,924 + Atu0308 0.53 +0.4 300,924 + Atu0308 0.53 +0.6 300,924 + Atu0308 0.53 +0.2 300,924 + Atu0308 0.53 -2.4 300,932 - Atu0308 0.54 -0.0 300,932 - Atu0308 0.54 +0.2 300,932 - Atu0308 0.54 -0.9 300,979 + Atu0308 0.58 -0.7 300,984 + Atu0308 0.58 +0.0 300,996 + Atu0308 0.60 +0.5 301,004 - Atu0308 0.60 +0.1 301,013 + Atu0308 0.61 -0.3 301,018 + Atu0308 0.62 -1.0 301,019 - Atu0308 0.62 -0.1 301,070 + Atu0308 0.66 +0.4 301,078 - Atu0308 0.67 -0.1 301,147 - Atu0308 0.73 +0.4 301,195 + Atu0308 0.78 +0.2 301,217 + Atu0308 0.80 -0.7 301,225 - Atu0308 0.80 +0.4 301,373 + +2.0 301,407 - -0.4 301,425 + +0.2 301,434 + -2.2 301,436 + -0.5 301,442 - +0.6 301,576 + -0.0 301,629 + Atu0309 0.15 -1.2 301,631 + Atu0309 0.16 +0.8 301,685 + Atu0309 0.26 +0.6 301,721 + Atu0309 0.33 +0.3 301,721 + Atu0309 0.33 -0.1 301,721 + Atu0309 0.33 -0.1 301,721 + Atu0309 0.33 +0.7 301,721 + Atu0309 0.33 +0.1 301,727 - Atu0309 0.35 +1.2 301,729 - Atu0309 0.35 +0.1 301,729 - Atu0309 0.35 +0.0 301,729 - Atu0309 0.35 -0.6 301,729 - Atu0309 0.35 +0.8 301,729 - Atu0309 0.35 -0.0 301,744 + Atu0309 0.38 -2.0 301,744 + Atu0309 0.38 -0.1 301,744 + Atu0309 0.38 -0.3 301,752 - Atu0309 0.39 -0.4 301,763 + Atu0309 0.42 +0.2 301,768 + Atu0309 0.42 -0.3 301,783 - Atu0309 0.45 +1.3 301,830 - Atu0309 0.55 +0.5 301,873 - Atu0309 0.63 +0.4 301,888 + Atu0309 0.66 +0.0 301,901 - Atu0309 0.68 -0.1 301,901 - Atu0309 0.68 +0.3 301,935 + Atu0309 0.75 -0.1 301,994 + Atu0309 0.87 -0.0 301,999 + Atu0309 0.88 -0.4 301,999 + Atu0309 0.88 +0.1 302,000 + Atu0309 0.88 +0.2 302,007 - Atu0309 0.89 -0.1 302,043 + +0.1 302,043 + -0.4 302,051 - -0.0 302,051 - -0.3 302,057 + -0.2 302,076 - +1.2 302,093 + -0.3 302,093 + -0.3 302,096 - +0.8 302,096 - +0.0 302,101 + +0.1 302,218 + +0.0 302,218 + +0.1 302,255 + -0.0
Or see this region's nucleotide sequence