Strain Fitness in Agrobacterium fabrum C58 around Atu0027

Experiment: R2A with Phosphomycin 50 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAtu0026 and Atu0027 overlap by 4 nucleotidesAtu0027 and Atu0028 overlap by 4 nucleotidesAtu0028 and Atu0029 are separated by 44 nucleotides Atu0026: Atu0026 - hypothetical protein, at 27,520 to 29,031 Atu0026 Atu0027: Atu0027 - two component sensor kinase, at 29,028 to 31,673 Atu0027 Atu0028: Atu0028 - hypothetical protein, at 31,670 to 31,837 Atu0028 Atu0029: Atu0029 - S-adenosylhomocysteine hydrolase, at 31,882 to 33,282 Atu0029 Position (kb) 29 30 31 32Strain fitness (log2 ratio) -1 0 1at 28.247 kb on - strand, within Atu0026at 32.177 kb on + strand, within Atu0029at 32.234 kb on + strand, within Atu0029at 32.236 kb on - strand, within Atu0029at 32.419 kb on + strand, within Atu0029at 32.534 kb on - strand, within Atu0029

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A with Phosphomycin 50 mM
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28,247 - Atu0026 0.48 -0.8
32,177 + Atu0029 0.21 -1.5
32,234 + Atu0029 0.25 -0.5
32,236 - Atu0029 0.25 -0.6
32,419 + Atu0029 0.38 +0.1
32,534 - Atu0029 0.47 +0.2

Or see this region's nucleotide sequence