Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rluC and yceF are separated by 111 nucleotides yceF and sraB overlap by 7 nucleotides sraB and yceD are separated by 36 nucleotides yceD and rpmF are separated by 51 nucleotides rpmF and plsX are separated by 80 nucleotides
b1086: rluC - 23S rRNA pseudouridylate synthase (NCBI), at 1,144,163 to 1,145,122
rluC
b1087: yceF - orf, hypothetical protein (VIMSS), at 1,145,234 to 1,145,818
yceF
b4418: sraB - unknown RNA (NCBI), at 1,145,812 to 1,145,980
sraB
b1088: yceD - hypothetical protein (NCBI), at 1,146,017 to 1,146,538
yceD
b1089: rpmF - 50S ribosomal protein L32 (NCBI), at 1,146,590 to 1,146,763
rpmF
b1090: plsX - glycerolphosphate auxotrophy in plsB background (VIMSS), at 1,146,844 to 1,147,914
plsX
Position (kb)
1145
1146 Strain fitness (log2 ratio)
-2
-1
0
1 at 1144.867 kb on + strand, within rluC at 1144.867 kb on + strand, within rluC at 1144.899 kb on + strand, within rluC at 1144.988 kb on + strand, within rluC at 1144.988 kb on + strand, within rluC at 1144.998 kb on + strand, within rluC at 1144.998 kb on + strand, within rluC at 1145.011 kb on - strand, within rluC at 1145.011 kb on - strand, within rluC at 1145.035 kb on + strand at 1145.058 kb on - strand at 1145.145 kb on - strand at 1145.279 kb on - strand at 1145.381 kb on + strand, within yceF at 1145.381 kb on + strand, within yceF at 1145.381 kb on + strand, within yceF at 1145.381 kb on + strand, within yceF at 1145.389 kb on - strand, within yceF at 1145.389 kb on - strand, within yceF at 1145.389 kb on - strand, within yceF at 1145.395 kb on + strand, within yceF at 1145.511 kb on - strand, within yceF at 1145.516 kb on - strand, within yceF at 1145.516 kb on - strand, within yceF at 1145.576 kb on - strand, within yceF at 1145.610 kb on - strand, within yceF at 1145.614 kb on - strand, within yceF at 1145.673 kb on - strand, within yceF at 1145.724 kb on - strand, within yceF at 1145.734 kb on - strand, within yceF at 1145.734 kb on - strand, within yceF at 1145.841 kb on + strand, within sraB at 1145.847 kb on - strand, within sraB at 1146.042 kb on + strand at 1146.052 kb on + strand at 1146.096 kb on + strand, within yceD at 1146.096 kb on + strand, within yceD at 1146.117 kb on + strand, within yceD at 1146.265 kb on + strand, within yceD at 1146.265 kb on + strand, within yceD at 1146.265 kb on + strand, within yceD at 1146.265 kb on + strand, within yceD at 1146.277 kb on + strand, within yceD at 1146.277 kb on + strand, within yceD at 1146.310 kb on + strand, within yceD at 1146.373 kb on + strand, within yceD at 1146.373 kb on + strand, within yceD
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,144,867 + rluC b1086 0.73 +0.6 1,144,867 + rluC b1086 0.73 +0.8 1,144,899 + rluC b1086 0.77 -0.1 1,144,988 + rluC b1086 0.86 -0.0 1,144,988 + rluC b1086 0.86 -0.0 1,144,998 + rluC b1086 0.87 +0.4 1,144,998 + rluC b1086 0.87 +0.6 1,145,011 - rluC b1086 0.88 -0.1 1,145,011 - rluC b1086 0.88 +0.3 1,145,035 + -0.4 1,145,058 - -0.8 1,145,145 - +0.2 1,145,279 - -0.1 1,145,381 + yceF b1087 0.25 +0.0 1,145,381 + yceF b1087 0.25 +0.2 1,145,381 + yceF b1087 0.25 -0.1 1,145,381 + yceF b1087 0.25 -0.3 1,145,389 - yceF b1087 0.26 +0.1 1,145,389 - yceF b1087 0.26 -0.5 1,145,389 - yceF b1087 0.26 +0.9 1,145,395 + yceF b1087 0.28 +0.7 1,145,511 - yceF b1087 0.47 -0.1 1,145,516 - yceF b1087 0.48 +0.3 1,145,516 - yceF b1087 0.48 +0.8 1,145,576 - yceF b1087 0.58 +0.5 1,145,610 - yceF b1087 0.64 +0.2 1,145,614 - yceF b1087 0.65 -0.3 1,145,673 - yceF b1087 0.75 -0.3 1,145,724 - yceF b1087 0.84 -0.4 1,145,734 - yceF b1087 0.85 -0.6 1,145,734 - yceF b1087 0.85 +1.4 1,145,841 + sraB b4418 0.17 -0.9 1,145,847 - sraB b4418 0.21 +1.0 1,146,042 + -0.7 1,146,052 + -0.4 1,146,096 + yceD b1088 0.15 +1.6 1,146,096 + yceD b1088 0.15 +0.7 1,146,117 + yceD b1088 0.19 -2.3 1,146,265 + yceD b1088 0.48 +0.3 1,146,265 + yceD b1088 0.48 +0.3 1,146,265 + yceD b1088 0.48 -1.7 1,146,265 + yceD b1088 0.48 -0.3 1,146,277 + yceD b1088 0.50 -0.4 1,146,277 + yceD b1088 0.50 +0.7 1,146,310 + yceD b1088 0.56 +0.3 1,146,373 + yceD b1088 0.68 +0.2 1,146,373 + yceD b1088 0.68 -0.0
Or see this region's nucleotide sequence