Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glnE and ygiF are separated by 22 nucleotides ygiF and htrG are separated by 241 nucleotides htrG and cca are separated by 63 nucleotides
b3053: glnE - fused deadenylyltransferase/adenylyltransferase for glutamine synthetase (NCBI), at 3,194,823 to 3,197,663
glnE
b3054: ygiF - predicted adenylate cyclase (NCBI), at 3,197,686 to 3,198,987
ygiF
b3055: htrG - predicted signal transduction protein (SH3 domain) (NCBI), at 3,199,229 to 3,199,849
htrG
b3056: cca - fused tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase and phosphatase (NCBI), at 3,199,913 to 3,201,151
cca
Position (kb)
3197
3198
3199 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3196.922 kb on - strand, within glnE at 3196.996 kb on - strand, within glnE at 3196.997 kb on - strand, within glnE at 3196.997 kb on - strand, within glnE at 3197.014 kb on - strand, within glnE at 3197.095 kb on + strand, within glnE at 3197.196 kb on - strand, within glnE at 3197.317 kb on + strand, within glnE at 3197.317 kb on + strand, within glnE at 3197.354 kb on - strand, within glnE at 3197.354 kb on - strand, within glnE at 3197.366 kb on + strand, within glnE at 3197.369 kb on + strand, within glnE at 3197.369 kb on + strand, within glnE at 3197.397 kb on - strand at 3197.397 kb on - strand at 3197.441 kb on + strand at 3197.515 kb on + strand at 3197.532 kb on + strand at 3197.540 kb on - strand at 3197.558 kb on + strand at 3197.558 kb on + strand at 3197.558 kb on + strand at 3197.600 kb on + strand at 3197.677 kb on - strand at 3197.701 kb on + strand at 3197.701 kb on + strand at 3197.701 kb on + strand at 3197.777 kb on + strand at 3197.780 kb on - strand at 3197.782 kb on + strand at 3197.820 kb on + strand, within ygiF at 3197.834 kb on - strand, within ygiF at 3197.899 kb on - strand, within ygiF at 3197.899 kb on - strand, within ygiF at 3197.903 kb on + strand, within ygiF at 3197.999 kb on + strand, within ygiF at 3197.999 kb on + strand, within ygiF at 3198.040 kb on - strand, within ygiF at 3198.113 kb on + strand, within ygiF at 3198.113 kb on - strand, within ygiF at 3198.113 kb on - strand, within ygiF at 3198.126 kb on + strand, within ygiF at 3198.130 kb on - strand, within ygiF at 3198.151 kb on - strand, within ygiF at 3198.151 kb on - strand, within ygiF at 3198.174 kb on + strand, within ygiF at 3198.282 kb on - strand, within ygiF at 3198.321 kb on + strand, within ygiF at 3198.393 kb on - strand, within ygiF at 3198.393 kb on - strand, within ygiF at 3198.398 kb on - strand, within ygiF at 3198.398 kb on - strand, within ygiF at 3198.491 kb on + strand, within ygiF at 3198.552 kb on + strand, within ygiF at 3198.560 kb on - strand, within ygiF at 3198.561 kb on - strand, within ygiF at 3198.561 kb on - strand, within ygiF at 3198.711 kb on + strand, within ygiF at 3198.733 kb on + strand, within ygiF at 3198.733 kb on + strand, within ygiF at 3198.750 kb on + strand, within ygiF at 3198.750 kb on + strand, within ygiF at 3198.758 kb on - strand, within ygiF at 3198.824 kb on - strand, within ygiF at 3198.824 kb on - strand, within ygiF at 3198.828 kb on - strand, within ygiF at 3198.898 kb on - strand at 3198.900 kb on - strand at 3198.919 kb on + strand at 3198.991 kb on - strand at 3198.998 kb on + strand at 3198.998 kb on + strand at 3199.136 kb on + strand at 3199.139 kb on - strand at 3199.175 kb on - strand at 3199.180 kb on + strand at 3199.214 kb on + strand at 3199.274 kb on - strand at 3199.310 kb on + strand, within htrG at 3199.327 kb on - strand, within htrG at 3199.446 kb on + strand, within htrG at 3199.446 kb on + strand, within htrG at 3199.480 kb on + strand, within htrG at 3199.480 kb on + strand, within htrG at 3199.488 kb on - strand, within htrG at 3199.578 kb on - strand, within htrG at 3199.716 kb on + strand, within htrG at 3199.716 kb on + strand, within htrG at 3199.716 kb on + strand, within htrG at 3199.716 kb on + strand, within htrG at 3199.721 kb on + strand, within htrG at 3199.721 kb on + strand, within htrG at 3199.724 kb on + strand, within htrG at 3199.724 kb on + strand, within htrG at 3199.747 kb on - strand, within htrG at 3199.759 kb on - strand, within htrG at 3199.759 kb on - strand, within htrG at 3199.771 kb on - strand, within htrG at 3199.771 kb on - strand, within htrG at 3199.778 kb on + strand, within htrG at 3199.781 kb on + strand, within htrG at 3199.781 kb on + strand, within htrG at 3199.878 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 3,196,922 - glnE b3053 0.74 -0.0 3,196,996 - glnE b3053 0.76 +0.5 3,196,997 - glnE b3053 0.77 +1.0 3,196,997 - glnE b3053 0.77 +0.8 3,197,014 - glnE b3053 0.77 +1.1 3,197,095 + glnE b3053 0.80 +0.3 3,197,196 - glnE b3053 0.84 +0.3 3,197,317 + glnE b3053 0.88 +0.0 3,197,317 + glnE b3053 0.88 +0.8 3,197,354 - glnE b3053 0.89 +0.4 3,197,354 - glnE b3053 0.89 +0.1 3,197,366 + glnE b3053 0.90 +0.0 3,197,369 + glnE b3053 0.90 -0.4 3,197,369 + glnE b3053 0.90 -2.2 3,197,397 - +0.4 3,197,397 - +0.4 3,197,441 + -1.2 3,197,515 + -0.3 3,197,532 + +0.7 3,197,540 - +0.4 3,197,558 + -0.0 3,197,558 + +0.6 3,197,558 + -0.0 3,197,600 + +0.2 3,197,677 - +0.6 3,197,701 + +0.8 3,197,701 + +0.9 3,197,701 + +1.0 3,197,777 + +0.5 3,197,780 - +0.6 3,197,782 + +0.3 3,197,820 + ygiF b3054 0.10 -1.1 3,197,834 - ygiF b3054 0.11 +0.1 3,197,899 - ygiF b3054 0.16 +0.6 3,197,899 - ygiF b3054 0.16 +0.8 3,197,903 + ygiF b3054 0.17 +1.2 3,197,999 + ygiF b3054 0.24 +0.2 3,197,999 + ygiF b3054 0.24 +0.9 3,198,040 - ygiF b3054 0.27 -0.9 3,198,113 + ygiF b3054 0.33 +1.0 3,198,113 - ygiF b3054 0.33 -0.3 3,198,113 - ygiF b3054 0.33 -0.1 3,198,126 + ygiF b3054 0.34 +0.1 3,198,130 - ygiF b3054 0.34 +0.8 3,198,151 - ygiF b3054 0.36 -0.2 3,198,151 - ygiF b3054 0.36 -0.3 3,198,174 + ygiF b3054 0.37 +0.1 3,198,282 - ygiF b3054 0.46 +0.3 3,198,321 + ygiF b3054 0.49 -0.4 3,198,393 - ygiF b3054 0.54 +0.4 3,198,393 - ygiF b3054 0.54 +0.2 3,198,398 - ygiF b3054 0.55 +1.0 3,198,398 - ygiF b3054 0.55 -0.6 3,198,491 + ygiF b3054 0.62 +0.2 3,198,552 + ygiF b3054 0.67 +1.0 3,198,560 - ygiF b3054 0.67 -0.1 3,198,561 - ygiF b3054 0.67 +0.6 3,198,561 - ygiF b3054 0.67 -1.2 3,198,711 + ygiF b3054 0.79 -0.9 3,198,733 + ygiF b3054 0.80 +0.3 3,198,733 + ygiF b3054 0.80 +0.2 3,198,750 + ygiF b3054 0.82 -0.7 3,198,750 + ygiF b3054 0.82 +0.3 3,198,758 - ygiF b3054 0.82 -0.0 3,198,824 - ygiF b3054 0.87 -0.3 3,198,824 - ygiF b3054 0.87 -0.1 3,198,828 - ygiF b3054 0.88 -0.2 3,198,898 - -0.2 3,198,900 - +0.3 3,198,919 + +0.9 3,198,991 - +0.6 3,198,998 + +0.4 3,198,998 + +0.7 3,199,136 + +1.1 3,199,139 - +0.9 3,199,175 - -0.2 3,199,180 + +0.3 3,199,214 + +1.8 3,199,274 - +0.4 3,199,310 + htrG b3055 0.13 +0.9 3,199,327 - htrG b3055 0.16 -0.2 3,199,446 + htrG b3055 0.35 +0.0 3,199,446 + htrG b3055 0.35 -1.3 3,199,480 + htrG b3055 0.40 +0.6 3,199,480 + htrG b3055 0.40 +0.7 3,199,488 - htrG b3055 0.42 +0.1 3,199,578 - htrG b3055 0.56 +0.1 3,199,716 + htrG b3055 0.78 +0.2 3,199,716 + htrG b3055 0.78 -0.1 3,199,716 + htrG b3055 0.78 +0.5 3,199,716 + htrG b3055 0.78 -0.2 3,199,721 + htrG b3055 0.79 -1.1 3,199,721 + htrG b3055 0.79 -0.7 3,199,724 + htrG b3055 0.80 -0.7 3,199,724 + htrG b3055 0.80 -0.5 3,199,747 - htrG b3055 0.83 -0.3 3,199,759 - htrG b3055 0.85 -0.5 3,199,759 - htrG b3055 0.85 +0.4 3,199,771 - htrG b3055 0.87 +1.0 3,199,771 - htrG b3055 0.87 +0.9 3,199,778 + htrG b3055 0.88 +0.6 3,199,781 + htrG b3055 0.89 -0.8 3,199,781 + htrG b3055 0.89 -0.5 3,199,878 - +0.4
Or see this region's nucleotide sequence