Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt potD and potC overlap by 4 nucleotides potC and potB overlap by 4 nucleotides potB and potA are separated by 13 nucleotides
b1123: potD - spermidine/putrescine ABC transporter periplasmic substrate-binding protein (NCBI), at 1,181,006 to 1,182,052
potD
b1124: potC - spermidine/putrescine ABC transporter membrane protein (NCBI), at 1,182,049 to 1,182,843
potC
b1125: potB - spermidine/putrescine ABC transporter membrane protein (NCBI), at 1,182,840 to 1,183,667
potB
b1126: potA - putrescine/spermidine ABC transporter ATPase protein (NCBI), at 1,183,681 to 1,184,817
potA
Position (kb)
1182
1183
1184 Strain fitness (log2 ratio)
-1
0
1 at 1181.865 kb on - strand, within potD at 1181.918 kb on + strand, within potD at 1182.003 kb on + strand at 1182.395 kb on + strand, within potC at 1182.474 kb on - strand, within potC at 1182.476 kb on - strand, within potC at 1182.524 kb on + strand, within potC at 1182.581 kb on + strand, within potC at 1182.581 kb on + strand, within potC at 1182.589 kb on + strand, within potC at 1182.668 kb on + strand, within potC at 1182.691 kb on + strand, within potC at 1182.700 kb on + strand, within potC at 1182.730 kb on + strand, within potC at 1182.910 kb on + strand at 1182.910 kb on + strand at 1182.914 kb on - strand at 1183.032 kb on + strand, within potB at 1183.032 kb on + strand, within potB at 1183.193 kb on - strand, within potB at 1183.202 kb on + strand, within potB at 1183.202 kb on + strand, within potB at 1183.283 kb on + strand, within potB at 1183.449 kb on + strand, within potB at 1183.542 kb on - strand, within potB at 1183.680 kb on - strand at 1183.698 kb on - strand at 1183.698 kb on - strand at 1183.711 kb on - strand at 1183.711 kb on - strand at 1183.884 kb on + strand, within potA at 1183.934 kb on - strand, within potA at 1184.015 kb on + strand, within potA at 1184.139 kb on - strand, within potA at 1184.163 kb on + strand, within potA at 1184.163 kb on + strand, within potA at 1184.286 kb on + strand, within potA at 1184.293 kb on + strand, within potA at 1184.334 kb on - strand, within potA at 1184.361 kb on - strand, within potA at 1184.599 kb on - strand, within potA at 1184.666 kb on + strand, within potA
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,181,865 - potD b1123 0.82 +0.4 1,181,918 + potD b1123 0.87 +0.9 1,182,003 + +0.6 1,182,395 + potC b1124 0.44 -0.9 1,182,474 - potC b1124 0.53 -0.1 1,182,476 - potC b1124 0.54 -0.2 1,182,524 + potC b1124 0.60 -0.1 1,182,581 + potC b1124 0.67 -0.6 1,182,581 + potC b1124 0.67 -0.3 1,182,589 + potC b1124 0.68 +1.0 1,182,668 + potC b1124 0.78 -0.3 1,182,691 + potC b1124 0.81 -0.9 1,182,700 + potC b1124 0.82 -0.6 1,182,730 + potC b1124 0.86 -0.3 1,182,910 + -0.7 1,182,910 + -0.2 1,182,914 - +0.5 1,183,032 + potB b1125 0.23 -0.4 1,183,032 + potB b1125 0.23 -0.0 1,183,193 - potB b1125 0.43 -0.6 1,183,202 + potB b1125 0.44 +0.1 1,183,202 + potB b1125 0.44 +0.8 1,183,283 + potB b1125 0.54 -1.5 1,183,449 + potB b1125 0.74 +0.9 1,183,542 - potB b1125 0.85 -1.5 1,183,680 - +0.6 1,183,698 - +0.4 1,183,698 - +1.2 1,183,711 - -0.7 1,183,711 - -0.1 1,183,884 + potA b1126 0.18 -0.5 1,183,934 - potA b1126 0.22 -0.5 1,184,015 + potA b1126 0.29 -0.2 1,184,139 - potA b1126 0.40 +0.1 1,184,163 + potA b1126 0.42 +0.8 1,184,163 + potA b1126 0.42 +0.9 1,184,286 + potA b1126 0.53 +0.0 1,184,293 + potA b1126 0.54 +0.0 1,184,334 - potA b1126 0.57 +0.2 1,184,361 - potA b1126 0.60 +0.4 1,184,599 - potA b1126 0.81 +0.3 1,184,666 + potA b1126 0.87 +0.0
Or see this region's nucleotide sequence