Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nagK and cobB are separated by 126 nucleotides cobB and ycfZ are separated by 119 nucleotides ycfZ and ymfA overlap by 4 nucleotides
b1119: nagK - N-acetyl-D-glucosamine kinase (NCBI), at 1,177,816 to 1,178,727
nagK
b1120: cobB - deacetylase of acs and cheY, regulates chemotaxis (NCBI), at 1,178,854 to 1,179,582
cobB
b1121: ycfZ - predicted inner membrane protein (NCBI), at 1,179,702 to 1,180,490
ycfZ
b1122: ymfA - orf, hypothetical protein (VIMSS), at 1,180,487 to 1,180,948
ymfA
Position (kb)
1178
1179
1180 Strain fitness (log2 ratio)
-1
0
1 at 1177.988 kb on - strand, within nagK at 1177.988 kb on - strand, within nagK at 1178.081 kb on + strand, within nagK at 1178.086 kb on + strand, within nagK at 1178.100 kb on + strand, within nagK at 1178.411 kb on - strand, within nagK at 1178.423 kb on + strand, within nagK at 1178.423 kb on + strand, within nagK at 1178.483 kb on + strand, within nagK at 1178.486 kb on - strand, within nagK at 1178.567 kb on - strand, within nagK at 1178.692 kb on - strand at 1178.693 kb on + strand at 1178.708 kb on + strand at 1178.708 kb on + strand at 1178.766 kb on + strand at 1178.766 kb on + strand at 1178.875 kb on + strand at 1178.875 kb on + strand at 1178.880 kb on - strand at 1178.880 kb on - strand at 1179.036 kb on - strand, within cobB at 1179.142 kb on + strand, within cobB at 1179.161 kb on + strand, within cobB at 1179.161 kb on + strand, within cobB at 1179.195 kb on - strand, within cobB at 1179.319 kb on + strand, within cobB at 1179.396 kb on + strand, within cobB at 1179.413 kb on + strand, within cobB at 1179.413 kb on + strand, within cobB at 1179.421 kb on - strand, within cobB at 1179.526 kb on + strand at 1179.552 kb on - strand at 1179.584 kb on + strand at 1179.584 kb on + strand at 1179.584 kb on + strand at 1179.594 kb on + strand at 1179.758 kb on - strand at 1179.768 kb on - strand at 1179.785 kb on + strand, within ycfZ at 1179.833 kb on + strand, within ycfZ at 1179.837 kb on - strand, within ycfZ at 1179.922 kb on + strand, within ycfZ at 1179.922 kb on + strand, within ycfZ at 1179.922 kb on + strand, within ycfZ at 1179.922 kb on + strand, within ycfZ at 1179.922 kb on + strand, within ycfZ at 1179.943 kb on + strand, within ycfZ at 1179.992 kb on + strand, within ycfZ at 1180.234 kb on - strand, within ycfZ at 1180.389 kb on - strand, within ycfZ at 1180.389 kb on - strand, within ycfZ at 1180.438 kb on + strand at 1180.457 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 1,177,988 - nagK b1119 0.19 +0.2 1,177,988 - nagK b1119 0.19 +0.1 1,178,081 + nagK b1119 0.29 +0.3 1,178,086 + nagK b1119 0.30 +0.1 1,178,100 + nagK b1119 0.31 +0.1 1,178,411 - nagK b1119 0.65 -0.5 1,178,423 + nagK b1119 0.67 +0.4 1,178,423 + nagK b1119 0.67 -0.1 1,178,483 + nagK b1119 0.73 -0.6 1,178,486 - nagK b1119 0.73 +0.6 1,178,567 - nagK b1119 0.82 +0.2 1,178,692 - +0.1 1,178,693 + -0.6 1,178,708 + +0.2 1,178,708 + +0.4 1,178,766 + +0.4 1,178,766 + +0.8 1,178,875 + +0.6 1,178,875 + +0.9 1,178,880 - +0.6 1,178,880 - -0.2 1,179,036 - cobB b1120 0.25 -1.1 1,179,142 + cobB b1120 0.40 +0.6 1,179,161 + cobB b1120 0.42 -0.1 1,179,161 + cobB b1120 0.42 -0.7 1,179,195 - cobB b1120 0.47 -0.8 1,179,319 + cobB b1120 0.64 -1.0 1,179,396 + cobB b1120 0.74 +0.2 1,179,413 + cobB b1120 0.77 -0.5 1,179,413 + cobB b1120 0.77 +0.9 1,179,421 - cobB b1120 0.78 -0.6 1,179,526 + -0.2 1,179,552 - +0.0 1,179,584 + +1.4 1,179,584 + -0.5 1,179,584 + -1.2 1,179,594 + -0.0 1,179,758 - +0.0 1,179,768 - -0.5 1,179,785 + ycfZ b1121 0.11 +0.3 1,179,833 + ycfZ b1121 0.17 +0.5 1,179,837 - ycfZ b1121 0.17 -0.5 1,179,922 + ycfZ b1121 0.28 +1.0 1,179,922 + ycfZ b1121 0.28 -0.0 1,179,922 + ycfZ b1121 0.28 -0.5 1,179,922 + ycfZ b1121 0.28 +0.5 1,179,922 + ycfZ b1121 0.28 +0.5 1,179,943 + ycfZ b1121 0.31 -0.3 1,179,992 + ycfZ b1121 0.37 +0.2 1,180,234 - ycfZ b1121 0.67 -1.6 1,180,389 - ycfZ b1121 0.87 +0.4 1,180,389 - ycfZ b1121 0.87 +0.5 1,180,438 + +0.4 1,180,457 + +0.0
Or see this region's nucleotide sequence