Experiment: LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt atoD and atoA overlap by 1 nucleotides atoA and atoE overlap by 4 nucleotides atoE and atoB are separated by 30 nucleotides
b2221: atoD - acetyl-CoA:acetoacetyl-CoA transferase, alpha subunit (NCBI), at 2,321,469 to 2,322,131
atoD
b2222: atoA - acetyl-CoA:acetoacetyl-CoA transferase, beta subunit (NCBI), at 2,322,131 to 2,322,781
atoA
b2223: atoE - short chain fatty acid transporter (NCBI), at 2,322,778 to 2,324,100
atoE
b2224: atoB - acetyl-CoA acetyltransferase (NCBI), at 2,324,131 to 2,325,315
atoB
Position (kb)
2322
2323
2324
2325 Strain fitness (log2 ratio)
-2
-1
0
1 at 2321.818 kb on + strand, within atoD at 2321.967 kb on + strand, within atoD at 2322.085 kb on - strand at 2322.255 kb on - strand, within atoA at 2322.362 kb on - strand, within atoA at 2322.391 kb on + strand, within atoA at 2322.410 kb on - strand, within atoA at 2322.423 kb on - strand, within atoA at 2322.629 kb on - strand, within atoA at 2322.892 kb on + strand at 2322.927 kb on + strand, within atoE at 2322.978 kb on - strand, within atoE at 2322.978 kb on - strand, within atoE at 2322.999 kb on + strand, within atoE at 2323.113 kb on - strand, within atoE at 2323.139 kb on + strand, within atoE at 2323.194 kb on - strand, within atoE at 2323.260 kb on + strand, within atoE at 2323.321 kb on - strand, within atoE at 2323.321 kb on - strand, within atoE at 2323.403 kb on + strand, within atoE at 2323.417 kb on - strand, within atoE at 2323.417 kb on - strand, within atoE at 2323.426 kb on - strand, within atoE at 2323.475 kb on - strand, within atoE at 2323.601 kb on + strand, within atoE at 2323.601 kb on + strand, within atoE at 2323.608 kb on + strand, within atoE at 2323.873 kb on + strand, within atoE at 2324.037 kb on + strand at 2324.037 kb on + strand at 2324.037 kb on + strand at 2324.148 kb on + strand at 2324.307 kb on - strand, within atoB at 2324.469 kb on + strand, within atoB at 2324.539 kb on - strand, within atoB at 2324.547 kb on + strand, within atoB at 2324.547 kb on + strand, within atoB at 2324.549 kb on + strand, within atoB at 2324.585 kb on - strand, within atoB at 2324.735 kb on + strand, within atoB at 2324.886 kb on - strand, within atoB at 2325.002 kb on - strand, within atoB at 2325.006 kb on - strand, within atoB at 2325.077 kb on - strand, within atoB at 2325.094 kb on + strand, within atoB
Per-strain Table
Position Strand Gene LocusTag Fraction LB remove 2,321,818 + atoD b2221 0.53 -0.4 2,321,967 + atoD b2221 0.75 +0.1 2,322,085 - +0.1 2,322,255 - atoA b2222 0.19 +0.3 2,322,362 - atoA b2222 0.35 +0.0 2,322,391 + atoA b2222 0.40 -0.0 2,322,410 - atoA b2222 0.43 -0.2 2,322,423 - atoA b2222 0.45 -0.3 2,322,629 - atoA b2222 0.76 +1.0 2,322,892 + +0.2 2,322,927 + atoE b2223 0.11 +0.7 2,322,978 - atoE b2223 0.15 +0.3 2,322,978 - atoE b2223 0.15 +0.6 2,322,999 + atoE b2223 0.17 +0.2 2,323,113 - atoE b2223 0.25 +0.3 2,323,139 + atoE b2223 0.27 +0.7 2,323,194 - atoE b2223 0.31 +0.5 2,323,260 + atoE b2223 0.36 +1.0 2,323,321 - atoE b2223 0.41 +0.5 2,323,321 - atoE b2223 0.41 +1.4 2,323,403 + atoE b2223 0.47 +0.8 2,323,417 - atoE b2223 0.48 +0.6 2,323,417 - atoE b2223 0.48 -2.3 2,323,426 - atoE b2223 0.49 +0.0 2,323,475 - atoE b2223 0.53 +1.3 2,323,601 + atoE b2223 0.62 -0.5 2,323,601 + atoE b2223 0.62 -0.2 2,323,608 + atoE b2223 0.63 +0.7 2,323,873 + atoE b2223 0.83 +1.0 2,324,037 + -0.5 2,324,037 + -1.0 2,324,037 + +0.5 2,324,148 + +0.3 2,324,307 - atoB b2224 0.15 +0.4 2,324,469 + atoB b2224 0.29 +0.3 2,324,539 - atoB b2224 0.34 -0.3 2,324,547 + atoB b2224 0.35 -1.6 2,324,547 + atoB b2224 0.35 +0.4 2,324,549 + atoB b2224 0.35 -0.2 2,324,585 - atoB b2224 0.38 +0.1 2,324,735 + atoB b2224 0.51 +0.5 2,324,886 - atoB b2224 0.64 +0.2 2,325,002 - atoB b2224 0.74 +0.3 2,325,006 - atoB b2224 0.74 -0.3 2,325,077 - atoB b2224 0.80 +0.7 2,325,094 + atoB b2224 0.81 +0.0
Or see this region's nucleotide sequence